##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781173_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15985
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.34576165154833	34.0	34.0	34.0	33.0	34.0
2	33.22496090084454	34.0	34.0	34.0	32.0	34.0
3	33.30053174851423	34.0	34.0	34.0	33.0	34.0
4	33.43672192680638	34.0	34.0	34.0	34.0	34.0
5	33.44404128870816	34.0	34.0	34.0	34.0	34.0
6	36.904723177979356	38.0	37.0	38.0	35.0	38.0
7	36.722552392868316	38.0	37.0	38.0	34.0	38.0
8	36.61063497028464	38.0	37.0	38.0	34.0	38.0
9	36.626149515170475	38.0	37.0	38.0	34.0	38.0
10-11	36.441851736002505	38.0	37.0	38.0	34.0	38.0
12-13	36.26703159211761	38.0	37.0	38.0	34.0	38.0
14-15	36.22571160462934	38.0	37.0	38.0	34.0	38.0
16-17	36.03681576477948	38.0	36.0	38.0	31.5	38.0
18-19	35.85989990616203	38.0	36.0	38.0	31.0	38.0
20-21	35.81019705974351	38.0	36.0	38.0	31.5	38.0
22-23	36.20275258054426	38.0	36.5	38.0	33.0	38.0
24-25	35.97863622145762	38.0	36.0	38.0	31.5	38.0
26-27	35.62946512355333	38.0	36.0	38.0	31.0	38.0
28-29	35.72180168908351	38.0	36.0	38.0	31.0	38.0
30-31	35.97916796997185	38.0	36.0	38.0	31.5	38.0
32-33	35.37629027213012	38.0	35.5	38.0	31.0	38.0
34-35	35.2410071942446	38.0	35.5	38.0	30.5	38.0
36-37	34.931029089771656	37.5	34.5	38.0	27.0	38.0
38-39	32.57707225523929	37.0	32.5	38.0	16.0	38.0
40-41	34.470034407256804	37.0	34.5	38.0	25.5	38.0
42-43	35.44795120425399	37.5	35.5	38.0	31.0	38.0
44-45	35.49790428526744	38.0	35.5	38.0	31.0	38.0
46-47	35.189052236471696	38.0	35.5	38.0	28.0	38.0
48-49	35.07641538942759	38.0	35.0	38.0	27.0	38.0
50-51	34.581545198623715	37.0	34.0	38.0	25.0	38.0
52-53	34.503127932436655	37.0	34.0	38.0	25.5	38.0
54-55	34.50181420081326	37.0	34.0	38.0	26.0	38.0
56-57	34.589490147012825	37.0	34.0	38.0	27.0	38.0
58-59	34.03371911166719	37.0	34.0	38.0	24.0	38.0
60-61	33.979824835783546	37.0	34.0	38.0	24.0	38.0
62-63	34.19268063809822	37.0	34.0	38.0	24.5	38.0
64-65	34.0844541757898	37.0	34.0	38.0	24.0	38.0
66-67	33.357553956834536	37.0	32.5	38.0	22.0	38.0
68-69	32.010353456365344	37.0	29.5	38.0	16.0	38.0
70-71	28.98848920863309	34.5	18.0	38.0	10.5	38.0
72-73	26.682765092274007	31.0	11.0	38.0	11.0	38.0
74-75	26.836972161401313	32.0	11.0	38.0	11.0	38.0
76	26.220081326243353	28.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	6.0
23	13.0
24	39.0
25	83.0
26	170.0
27	310.0
28	490.0
29	572.0
30	774.0
31	966.0
32	1235.0
33	1514.0
34	1930.0
35	2555.0
36	1690.0
37	3636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.27807319361902	9.50891460744448	9.840475445730371	57.37253675320613
2	13.049734125742884	9.271191742258367	57.14106975289334	20.538004379105413
3	13.224898342195809	10.259618392242729	56.43415702220832	20.081326243353143
4	10.634970284641852	46.51861119799812	20.481701595245543	22.364716922114482
5	11.204253988113857	20.8007507037848	55.73350015639662	12.261495151704723
6	20.70691273068502	55.695964967156705	11.37941820456678	12.217704097591492
7	18.648733187363153	17.47888645605255	11.285580231467	52.58680012511729
8	16.027525805442604	56.79074131998749	9.146074444791992	18.035658429777918
9	14.238348451673444	12.761964341570224	8.845792930872694	64.15389427588364
10-11	17.347513293712854	33.193619017829214	30.69127306850172	18.76759461995621
12-13	16.08695652173913	9.94369721614013	34.388489208633096	39.58085705348765
14-15	16.008758210822645	9.593368783234283	13.030966531122928	61.36690647482015
16-17	38.192055051610886	30.556771973725365	9.40256490459806	21.848608070065687
18-19	39.3149827963716	14.407256803253048	9.724741945573975	36.553018454801375
20-21	17.635283077885518	10.4003753518924	10.028151391929935	61.93619017829215
22-23	16.709415076634347	32.915233030966526	9.274319674695027	41.101032217704095
24-25	38.59555833593994	31.84548013762903	9.596496715670941	19.96246481076009
26-27	39.427588364091335	34.53237410071942	10.284641851736003	15.755395683453239
28-29	17.663434469815453	34.022521113543945	10.090710040663122	38.22333437597748
30-31	15.943071629652799	35.91804817015952	10.156396621832968	37.98248357835471
32-33	37.17547700969659	11.967469502658743	10.807006568658117	40.050046918986546
34-35	17.306850172036285	30.73819205505161	10.73506412261495	41.21989365029715
36-37	15.74601188614326	33.69096027525805	32.60556771973725	17.95746011886143
38-39	15.821082264623085	14.69502658742571	32.42414763841101	37.05974350954019
40-41	15.520800750703783	10.431654676258994	34.738817641538944	39.30872693149828
42-43	37.68532999687207	10.062558648733187	32.952768220206444	19.2993431341883
44-45	16.606193306224583	31.379418204566782	35.383171723490776	16.63121676571786
46-47	37.17234907725993	11.567094150766344	15.498905223647169	35.761651548326554
48-49	35.614638723803566	9.996872067563341	12.564904598060684	41.82358461057241
50-51	17.672818267125432	10.34407256803253	34.422896465436345	37.560212699405696
52-53	14.35095401939318	10.087582108226462	55.1579605880513	20.40350328432906
54-55	13.922427275570847	10.303409446355959	36.9596496715671	38.814513606506104
56-57	36.10885204879575	10.15014075695965	16.556146387238037	37.18486080700657
58-59	15.633406318423523	11.53581482639975	52.60243978730059	20.228339067876135
60-61	35.56146387238036	31.49202377228652	16.493587738504846	16.452924616828277
62-63	14.9577729121051	57.04097591492023	11.542070691273068	16.459180481701598
64-65	13.803565842977791	58.70816390365968	10.719424460431656	16.768845792930872
66-67	13.518923991241788	58.645605254926494	10.441038473568971	17.39443228026275
68-69	13.778542383484515	57.37253675320613	10.928995933687832	17.91992492962152
70-71	15.411323115420709	47.891773537691584	14.817015952455426	21.87988739443228
72-73	17.691585861745388	32.53675320613075	20.40975914920238	29.36190178292149
74-75	18.41726618705036	28.579918673756648	22.20519236784485	30.797622771348138
76	18.492336565530184	29.44635595871129	21.294964028776977	30.766343446981548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	3.5
17	3.0
18	3.0
19	5.0
20	10.0
21	13.0
22	12.5
23	22.5
24	28.0
25	23.0
26	36.0
27	46.0
28	48.5
29	54.0
30	45.0
31	68.0
32	94.0
33	88.0
34	79.0
35	95.0
36	121.5
37	123.0
38	117.0
39	134.0
40	471.5
41	1298.5
42	1811.0
43	2017.0
44	2037.0
45	1496.5
46	1142.0
47	879.5
48	485.5
49	291.0
50	228.0
51	192.0
52	144.0
53	136.0
54	140.0
55	146.0
56	152.5
57	172.5
58	192.0
59	205.0
60	229.0
61	261.5
62	283.0
63	280.0
64	283.0
65	306.0
66	341.0
67	359.0
68	332.5
69	325.5
70	357.0
71	369.0
72	356.0
73	353.0
74	331.0
75	299.0
76	271.5
77	241.5
78	206.5
79	174.0
80	151.5
81	109.0
82	68.0
83	47.0
84	35.5
85	23.0
86	15.0
87	8.0
88	6.5
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	15985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.194870190803876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80865603644646	31.229277447607135
2	16.15793470007593	13.312480450422271
3	5.254365983295369	6.493587738504848
4	1.4882308276385725	2.4522990303409444
5	0.42520880789673504	0.8758210822646231
6	0.24297646165527714	0.6005630278385987
7	0.060744115413819286	0.17516421645292463
8	0.030372057706909643	0.10009383797309977
9	0.04555808656036447	0.16890835157960588
>10	0.36446469248291574	3.077885517672818
>50	0.030372057706909643	1.1323115420706913
>100	0.060744115413819286	5.430090710040663
>500	0.015186028853454821	3.278073193619018
>1k	0.0	0.0
>5k	0.015186028853454821	31.67344385361276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT	5063	31.67344385361276	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	524	3.278073193619018	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT	304	1.9017829214888957	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	250	1.563966218329684	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT	186	1.163590866437285	No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT	128	0.8007507037847982	Illumina PCR Primer Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	97	0.6068188927119175	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAGCTCGTATGCCGT	84	0.5254926493587738	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATCTCGTATGCCGTCT	46	0.28776978417266186	RNA PCR Primer, Index 41 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC	45	0.28151391929934316	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	40	0.25023459493274947	No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC	33	0.2064435408195183	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGC	30	0.1876759461995621	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATATCGTATGCCGT	29	0.18142008132624335	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATTTCGTATGCCGT	25	0.15639662183296843	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	23	0.14388489208633093	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCG	23	0.14388489208633093	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCCCGTATGCCGT	22	0.13762902721301218	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCC	16	0.10009383797309977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.006255864873318737	0.0
5	0.0	0.0	0.0	0.006255864873318737	0.0
6	0.0	0.0	0.0	0.006255864873318737	0.0
7	0.0	0.0	0.0	0.006255864873318737	0.0
8	0.0	0.0	0.0	0.006255864873318737	0.0
9	0.0	0.0	0.0	0.006255864873318737	0.0
10	0.0	0.0	0.0	0.006255864873318737	0.0
11	0.0	0.0	0.0	0.006255864873318737	0.0
12	0.0	0.0	0.0	0.012511729746637473	0.0
13	0.0	0.0	0.0	0.012511729746637473	0.0
14	0.0	0.0	0.0	0.012511729746637473	0.0
15	0.0	0.0	0.0	0.025023459493274947	0.0
16	0.0	0.0	0.0	0.025023459493274947	0.0
17	0.0	0.0	0.0	0.025023459493274947	0.0
18	0.0	0.0	0.0	0.025023459493274947	0.0
19	0.0	0.0	0.0	0.025023459493274947	0.0
20	0.0	0.0	0.0	0.05004691898654989	0.0
21	0.0	0.0	0.0	0.05630278385986863	0.0
22	0.0	0.0	0.0	0.05630278385986863	0.0
23	0.0	0.0	0.0	0.05630278385986863	0.0
24	0.0	0.0	0.0	0.0688145136065061	0.0
25	0.0	0.0	0.0	0.07507037847982484	0.0
26	0.0	0.0	0.0	0.07507037847982484	0.0
27	0.0	0.0	0.0	0.08132624335314358	0.0
28	0.0	0.0	0.0	0.12511729746637473	0.0
29	0.0	0.0	0.0	0.20018767594619957	0.0
30	0.0	0.0	0.0	0.237722865186112	0.0
31	0.0	0.0	0.0	0.2564904598060682	0.0
32	0.0	0.0	0.0	0.2940256490459806	0.0
33	0.0	0.0	0.0	0.36909602752580545	0.0
34	0.0	0.0	0.0	0.5129809196121364	0.0
35	0.0	0.0	0.0	0.7006568658116985	0.0
36	0.0	0.0	0.0	0.9008445417578981	0.0
37	0.0	0.0	0.0	1.207381920550516	0.0
38	0.0	0.0	0.0	1.5577103534563654	0.0
39	0.0	0.0	0.0	1.901782921488896	0.0
40	0.0	0.0	0.0	2.2959024085079762	0.0
41	0.0	0.0	0.0	2.7400688145136063	0.0
42	0.0	0.0	0.0	3.084141382546137	0.0
43	0.0	0.0	0.0	3.5408195182984046	0.0
44	0.0	0.0	0.0	4.0538004379105415	0.0
45	0.0	0.0	0.0	4.4979668439161715	0.0
46	0.0	0.0	0.0	4.954644979668439	0.0
47	0.0	0.0	0.0	5.367532061307476	0.0
48	0.0	0.0	0.0	5.730372223959963	0.0
49	0.0	0.0	0.0	6.180794494838912	0.0
50	0.0	0.0	0.0	6.5624022521113545	0.0
51	0.0	0.0	0.0	6.918986549890523	0.0
52	0.0	0.0	0.0	7.2442915233030964	0.0
53	0.0	0.0	0.0	7.507037847982484	0.0
54	0.0	0.0	0.0	7.857366280888333	0.0
55	0.0	0.0	0.0	8.188927119174226	0.0
56	0.0	0.0	0.0	8.52048795746012	0.0
57	0.0	0.0	0.0	8.783234282139507	0.0
58	0.0	0.0	0.0	9.133562715045356	0.0
59	0.0	0.0	0.0	9.446355958711292	0.0
60	0.0	0.0	0.0	9.777916796997184	0.0
61	0.0	0.0	0.0	10.059430716296529	0.0
62	0.0	0.0	0.0	10.340944635595871	0.0
63	0.0	0.0	0.0	10.509852987175478	0.0
64	0.0	0.0	0.0	10.866437284954644	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGACA	15	0.0021847868	70.00001	57
ACTCGTA	25	2.7145888E-6	70.0	38
ATAACTC	25	2.7145888E-6	70.0	35
AACTCGT	25	2.7145888E-6	70.0	37
TAACTCG	25	2.7145888E-6	70.0	36
GCCATAA	25	2.7145888E-6	70.0	32
CCATAAC	25	2.7145888E-6	70.0	33
CCATACC	35	2.7929127E-7	60.0	33
ATACCTC	35	2.7929127E-7	60.0	35
CATACCT	35	2.7929127E-7	60.0	34
CATAACT	30	8.009023E-6	58.333336	34
TTCTGCT	770	0.0	56.81818	52
TTGAAAA	765	0.0	56.73203	58
GCCGTCT	765	0.0	56.73203	46
CTTGAAA	765	0.0	56.73203	57
CCGTCTT	760	0.0	56.64474	47
TCTTCTG	760	0.0	56.64474	50
CTGCTTG	785	0.0	56.624203	54
CGCCATA	805	0.0	56.52174	31
CTCGTAT	805	0.0	56.52174	39
>>END_MODULE