FastQCFastQC Report
Wed 25 May 2016
SRR1781172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326093
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA200.006947593852.511
ATCGATA200.006947593852.523
CCCAACG459.1874055E-538.88888521
CTACGGG459.1874055E-538.88888538
CGGGCTA459.1874055E-538.88888541
CGCAAAG459.1874055E-538.88888512
TTTACGG501.7078024E-435.00000436
TACGGAC501.7078024E-435.00000438
TATCCAC751.4874968E-632.66666865
TGGCGTA450.004303980631.11110967
GGACTAG1401.2732926E-1130.01
CGTGCGA604.9765577E-429.16666610
CTCGCTA604.9765577E-429.16666613
CCAACGT604.9765577E-429.16666622
CGATTAG500.007202914528.00000252
TTGCGAT500.007202914528.00000249
CGATCAA500.007202914528.00000235
GACTAGG1004.4082662E-728.0000022
TCACCGG657.9470477E-426.92307731
TAACGGC657.9470477E-426.92307736