##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781172_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326093
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63187188930765	34.0	33.0	34.0	24.0	34.0
2	31.590494122842255	34.0	33.0	34.0	24.0	34.0
3	31.4947085647346	34.0	33.0	34.0	23.0	34.0
4	31.681734965178645	34.0	34.0	34.0	23.0	34.0
5	31.721245166256253	34.0	34.0	34.0	23.0	34.0
6	34.53022910642056	37.0	34.0	38.0	26.0	38.0
7	34.45755965322776	37.0	34.0	38.0	25.0	38.0
8	34.233439540253855	37.0	34.0	38.0	24.0	38.0
9	34.594713777971315	38.0	35.0	38.0	26.0	38.0
10-11	34.466837681274974	38.0	34.0	38.0	25.5	38.0
12-13	34.430863281333856	38.0	34.0	38.0	25.0	38.0
14-15	34.316343803761505	37.5	34.0	38.0	24.5	38.0
16-17	34.290644080063046	37.5	34.0	38.0	24.5	38.0
18-19	34.34553026283913	37.5	34.0	38.0	24.5	38.0
20-21	34.27495683746662	37.0	34.0	38.0	24.5	38.0
22-23	34.3435063003499	37.5	34.0	38.0	24.5	38.0
24-25	34.32847684556246	37.5	34.0	38.0	24.5	38.0
26-27	34.25147120606698	37.0	34.0	38.0	24.0	38.0
28-29	34.33718141757106	37.0	34.0	38.0	24.5	38.0
30-31	34.410229597078136	38.0	34.0	38.0	25.0	38.0
32-33	34.372278767100184	37.5	34.0	38.0	24.5	38.0
34-35	34.365811286964146	38.0	34.0	38.0	24.5	38.0
36-37	34.23442852192473	37.0	34.0	38.0	24.0	38.0
38-39	34.24748001337042	37.0	34.0	38.0	24.0	38.0
40-41	34.24527665420601	37.0	34.0	38.0	24.0	38.0
42-43	34.323941329620695	37.0	34.0	38.0	24.5	38.0
44-45	34.320942185204835	37.0	34.0	38.0	24.5	38.0
46-47	34.32060945803805	37.0	34.0	38.0	24.5	38.0
48-49	34.280389336784296	37.0	34.0	38.0	24.5	38.0
50-51	34.16613052104768	37.0	34.0	38.0	24.0	38.0
52-53	34.25014489731457	37.0	34.0	38.0	24.0	38.0
54-55	34.20662970379615	37.0	34.0	38.0	24.0	38.0
56-57	34.18139610479219	37.0	34.0	38.0	24.0	38.0
58-59	34.180083595784026	37.0	34.0	38.0	24.0	38.0
60-61	34.16060449013012	37.0	34.0	38.0	24.0	38.0
62-63	34.12365030834762	37.0	34.0	38.0	24.0	38.0
64-65	34.16122854523096	37.0	34.0	38.0	24.0	38.0
66-67	34.05447372375365	37.0	34.0	38.0	24.0	38.0
68-69	33.836483763834245	37.0	34.0	38.0	23.5	38.0
70-71	33.9744965393308	37.0	34.0	38.0	24.0	38.0
72-73	33.957714517024286	37.0	34.0	38.0	23.5	38.0
74-75	33.96865618090544	37.0	34.0	38.0	24.0	38.0
76	32.62284685657159	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	26.0
14	119.0
15	334.0
16	792.0
17	1495.0
18	2058.0
19	2257.0
20	2299.0
21	2355.0
22	2600.0
23	2943.0
24	3636.0
25	4097.0
26	5040.0
27	5932.0
28	7098.0
29	8763.0
30	10398.0
31	12480.0
32	15284.0
33	19102.0
34	24218.0
35	32677.0
36	50152.0
37	109935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.91915189838482	17.980760089913	9.01797953344598	25.082108478256202
2	24.130539447335575	20.030482101731714	31.019985096276216	24.81899335465649
3	19.991842817846443	21.42824286323227	29.443747642543695	29.136166676377595
4	14.359400539110009	16.46186824004195	36.54264274302116	32.63608847782688
5	13.928848518674119	33.66738936438378	36.03143888399935	16.372323232942747
6	30.35821069449513	36.63586768191896	18.55452278951097	14.451398834074942
7	29.36708239673958	29.245031325419436	21.928713587841507	19.45917268999948
8	25.875440441837146	33.7348547806914	19.743447421441125	20.646257356030333
9	25.036415991756954	15.820640124136364	21.162367790783613	37.98057609332307
10-11	25.56893278911231	24.17684525580126	27.485870595198303	22.768351359888133
12-13	25.639771476235307	22.5216425988905	26.646846145118108	25.191739779756084
14-15	23.792292382847837	23.577016372629895	23.88245071191347	28.748240532608794
16-17	24.152925699110376	26.547181325572765	25.05956889598979	24.240324079327063
18-19	23.548190240207546	26.526175048222438	26.035824136059343	23.889810575510666
20-21	23.421232593155942	26.47603597746655	26.152968631648026	23.94976279772948
22-23	23.95865596624276	25.89798002410355	26.30093255604996	23.842431453603727
24-25	23.712100535736738	26.11049608547255	26.134722303146646	24.042681075644065
26-27	22.808370618197877	26.337425213052718	26.59778038780348	24.256423780945926
28-29	23.492684602245372	25.88280030543434	27.264921356790854	23.359593735529437
30-31	23.823418472644306	25.919446292928704	26.452883073233707	23.80425216119328
32-33	23.15060427546743	26.464076199121113	26.469749427310614	23.91557009810085
34-35	23.98686877669867	25.82913463337146	26.534608225260893	23.649388364668976
36-37	23.94562287445606	25.76780243672817	26.151588657223552	24.134986031592216
38-39	23.26882208449737	26.255086739059102	26.852309003873128	23.6237821725704
40-41	23.400532976788828	25.378802979518113	27.50841017746471	23.712253866228348
42-43	23.16256405381287	25.547159859303942	27.025265798407204	24.265010288475988
44-45	22.681872962621092	25.616618572002466	27.180436255914724	24.521072209461718
46-47	23.009386892696256	25.148040589647742	27.40690539202007	24.435667125635938
48-49	22.35343904959628	25.09621488348416	28.172331206128316	24.378014860791247
50-51	22.373678674488566	25.131174235570835	27.987261302757187	24.50788578718341
52-53	22.01350534970085	25.25138534099168	28.153164894677285	24.581944414630183
54-55	21.42379627897563	25.6041988021822	28.160218097291263	24.811786821550907
56-57	21.39350244716215	25.55459808888289	28.246936447382947	24.80496301657201
58-59	21.50199482969582	25.440901828619445	27.838377395405605	25.21872594627913
60-61	21.37629660295775	25.19729831259535	27.812507187377815	25.613897897069084
62-63	21.054181028666758	24.962433914355458	28.341388319860656	25.641996737117132
64-65	21.501414475953908	25.11373306653787	27.880126037857355	25.504726419650865
66-67	21.646618674149206	24.96454226944809	27.367694749189265	26.021144307213444
68-69	21.398869030825658	25.171270684346748	27.124707137862934	26.30515314696466
70-71	21.8462552803269	25.136579344817804	27.145211864731632	25.871953510123664
72-73	21.91942764618574	24.821982753333415	26.955736417943406	26.302853182537444
74-75	21.8469021012475	25.394060571863154	26.665787569152265	26.09324975773708
76	22.10075039942593	25.539646665215137	26.317032257668828	26.042570677690108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	8.0
14	12.0
15	18.0
16	39.0
17	52.0
18	72.5
19	129.5
20	214.0
21	262.0
22	324.0
23	499.5
24	751.5
25	890.0
26	1134.0
27	1506.5
28	2009.0
29	2383.0
30	2748.5
31	3546.5
32	4838.0
33	5697.0
34	6266.0
35	7483.5
36	9229.5
37	10327.0
38	11381.5
39	13689.0
40	16178.0
41	18118.0
42	18822.0
43	18947.5
44	19594.5
45	19832.5
46	19549.0
47	19489.0
48	18261.5
49	16352.0
50	15610.0
51	14567.5
52	12287.0
53	10387.0
54	9725.0
55	9110.5
56	7874.0
57	6842.5
58	6433.0
59	5865.0
60	4961.0
61	4378.5
62	4132.0
63	3967.0
64	3682.5
65	3484.5
66	3304.5
67	3203.0
68	3092.5
69	2816.5
70	2578.0
71	2505.0
72	2419.0
73	2213.0
74	1963.5
75	1834.0
76	1748.0
77	1480.0
78	1211.0
79	1124.0
80	954.0
81	694.5
82	518.5
83	432.0
84	375.5
85	272.5
86	158.0
87	90.0
88	77.5
89	52.5
90	33.5
91	22.0
92	17.0
93	11.5
94	5.0
95	3.0
96	2.0
97	1.0
98	1.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.066609832164444E-4
58-59	0.0
60-61	1.533304916082222E-4
62-63	3.066609832164444E-4
64-65	1.533304916082222E-4
66-67	1.533304916082222E-4
68-69	3.066609832164444E-4
70-71	1.533304916082222E-4
72-73	3.066609832164444E-4
74-75	3.066609832164444E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	326093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.21492645308349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.818516613816	34.85328086665426
2	14.792709590972395	14.560442291230583
3	6.06877227161905	8.96022543024732
4	2.5686735382006316	5.056683170581034
5	1.2077019684389012	2.9718481776982344
6	0.7102708796029024	2.0973557460854257
7	0.5033623427381272	1.7341058473976114
8	0.38781447083965476	1.5269008527852082
9	0.34058672587696426	1.5085755598438173
>10	2.4884100940701375	23.088515535460722
>50	0.10820649266816067	3.3546799283878235
>100	0.004975011157156813	0.28738659362796887
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.199829496493332E-4	0.0
2	0.0	0.0	0.0	9.199829496493332E-4	0.0
3	0.0	0.0	0.0	0.0012266439328657776	0.0
4	0.0	0.0	0.0	0.002146626882515111	0.0
5	0.0	0.0	0.0	0.002146626882515111	0.0
6	0.0	0.0	0.0	0.002146626882515111	0.0
7	0.0	0.0	0.0	0.002146626882515111	0.0
8	0.0	0.0	0.0	0.002146626882515111	0.0
9	0.0	0.0	0.0	0.002453287865731555	0.0
10	0.0	0.0	0.0	0.002453287865731555	0.0
11	0.0	0.0	0.0	0.002453287865731555	0.0
12	0.0	0.0	0.0	0.002453287865731555	0.0
13	0.0	0.0	0.0	0.0030666098321644438	0.0
14	0.0	0.0	0.0	0.004599914748246666	0.0
15	0.0	0.0	0.0	0.00490657573146311	0.0
16	0.0	0.0	0.0	0.00490657573146311	0.0
17	0.0	0.0	0.0	0.005519897697895999	0.0
18	0.0	0.0	0.0	0.0061332196643288876	0.0
19	0.0	0.0	0.0	0.006746541630761776	0.0
20	0.0	0.0	0.0	0.007053202613978221	0.0
21	0.0	0.0	0.0	0.007359863597194666	0.0
22	0.0	0.0	0.0	0.009506490479709776	0.0
23	0.0	0.0	0.0	0.010733134412575554	0.0
24	0.0	0.0	0.0	0.012266439328657775	0.0
25	0.0	0.0	0.0	0.012879761295090664	0.0
26	0.0	0.0	0.0	0.013186422278307108	0.0
27	0.0	0.0	0.0	0.019932963909068884	0.0
28	0.0	0.0	0.0	0.03219940323772666	0.0
29	0.0	0.0	0.0	0.045692486499250215	0.0
30	0.0	0.0	0.0	0.05857224779434088	0.0
31	0.0	0.0	0.0	0.07175867007264798	0.0
32	0.0	0.0	0.0	0.09107831201528398	0.0
33	0.0	0.0	0.0	0.1272643080348244	0.0
34	0.0	0.0	0.0	0.19258309745992708	0.0
35	0.0	0.0	0.0	0.28090146062626303	0.0
36	0.0	0.0	0.0	0.40509915882892306	0.0
37	0.0	0.0	0.0	0.6062687638189106	0.0
38	0.0	0.0	0.0	0.8868635634619572	0.0
39	0.0	0.0	0.0	1.1699116509707352	0.0
40	0.0	0.0	0.0	1.5244117475689452	0.0
41	0.0	0.0	0.0	1.8976181641433578	0.0
42	0.0	0.0	0.0	2.305477271821229	0.0
43	0.0	0.0	0.0	2.787241676454263	0.0
44	0.0	0.0	0.0	3.3659109517836936	0.0
45	0.0	0.0	0.0	4.031058624380162	0.0
46	0.0	0.0	0.0	4.780844728344368	0.0
47	0.0	0.0	0.0	5.568656794227413	0.0
48	0.0	0.0	0.0	6.353402250278295	0.0
49	0.0	0.0	0.0	7.0706822900215585	0.0
50	0.0	0.0	0.0	7.68983081513556	0.0
51	0.0	0.0	0.0	8.316339203846756	0.0
52	0.0	0.0	0.0	8.93180779716216	0.0
53	0.0	0.0	0.0	9.542063153762884	0.0
54	0.0	0.0	0.0	10.224690502402689	0.0
55	0.0	0.0	0.0	10.987356367661986	0.0
56	0.0	0.0	0.0	11.718129490666772	0.0
57	0.0	0.0	0.0	12.445222681872963	0.0
58	0.0	0.0	0.0	13.150849604254	0.0
59	0.0	0.0	0.0	13.830103682078425	0.0
60	0.0	0.0	0.0	14.521317538248292	0.0
61	0.0	0.0	0.0	15.153039163674167	0.0
62	0.0	0.0	0.0	15.765747808140622	0.0
63	0.0	0.0	0.0	16.37998975752316	0.0
64	0.0	0.0	0.0	17.027044432109857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	20	0.0069475938	52.5	11
ATCGATA	20	0.0069475938	52.5	23
CCCAACG	45	9.1874055E-5	38.888885	21
CTACGGG	45	9.1874055E-5	38.888885	38
CGGGCTA	45	9.1874055E-5	38.888885	41
CGCAAAG	45	9.1874055E-5	38.888885	12
TTTACGG	50	1.7078024E-4	35.000004	36
TACGGAC	50	1.7078024E-4	35.000004	38
TATCCAC	75	1.4874968E-6	32.666668	65
TGGCGTA	45	0.0043039806	31.111109	67
GGACTAG	140	1.2732926E-11	30.0	1
CGTGCGA	60	4.9765577E-4	29.166666	10
CTCGCTA	60	4.9765577E-4	29.166666	13
CCAACGT	60	4.9765577E-4	29.166666	22
CGATTAG	50	0.0072029145	28.000002	52
TTGCGAT	50	0.0072029145	28.000002	49
CGATCAA	50	0.0072029145	28.000002	35
GACTAGG	100	4.4082662E-7	28.000002	2
TCACCGG	65	7.9470477E-4	26.923077	31
TAACGGC	65	7.9470477E-4	26.923077	36
>>END_MODULE