FastQCFastQC Report
Wed 25 May 2016
SRR1781172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291952
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT42221.446128130651614No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4610.15790266893187924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5250.057.33333238
CGTTATG252.3880739E-455.99999638
ATCGATA252.3880739E-455.99999623
ATCTCGT5450.055.8715637
CTCGTAT6100.055.65573539
TCGTATG6300.055.55555740
TATGCCG6350.055.1181143
CCGTCTT6300.054.44444747
GCCGTCT6350.054.01574746
CATCTCG5700.053.42105536
CGTCTTC6300.053.33333648
CGTATGC6600.053.03030441
TGCCGTC6650.052.6315845
GTTACGG200.006946868752.50000447
CGGGATT200.006946868752.50000469
GTATGCC6800.051.47059242
ATGCCGT6950.050.8633144
GTCTTCT6650.050.52631849
TCTTCTG6900.048.69565250
ATGCCAT6400.048.6718832
Produced by FastQC (version 0.11.5)