FastQCFastQC Report
Wed 25 May 2016
SRR1781171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190532
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTA150.002222048670.01
CGAGAAT308.353398E-658.33333214
TCGTAAA200.00694318252.52
CGTAAAC200.00694318252.53
GTTTCAC305.866993E-446.66666443
CGTGCGA404.5803146E-543.7510
CCGAAGC459.1730224E-538.8888853
GATGTAT501.7051422E-435.00000424
TGCGATG501.7051422E-435.00000429
TGTGCGC400.002414387435.04
ATCGGGC552.9841546E-431.81818444
ACAAGGT552.9841546E-431.8181845
CTTGTTG450.004299531731.11110913
CAACGGC450.004299531731.11110948
AGGCGAG450.004299531731.11110945
ATACTGG450.004299531731.1111096
TAGGGAT450.004299531731.1111095
AGCCTTA450.004299531731.1111096
TACACCG450.004299531731.1111095
CAGATAA604.968884E-429.16666614
Produced by FastQC (version 0.11.5)