##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781171_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	190532
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.377039027564923	34.0	33.0	34.0	23.0	34.0
2	31.450695946087798	34.0	33.0	34.0	23.0	34.0
3	31.39497302290429	34.0	33.0	34.0	23.0	34.0
4	31.564193941175237	34.0	34.0	34.0	23.0	34.0
5	31.5164171897634	34.0	34.0	34.0	23.0	34.0
6	34.32364642159847	37.0	34.0	38.0	24.0	38.0
7	34.25995633279449	37.0	34.0	38.0	24.0	38.0
8	34.183633195473725	37.0	34.0	38.0	24.0	38.0
9	34.48048096907606	38.0	35.0	38.0	26.0	38.0
10-11	34.314314655805845	38.0	34.0	38.0	24.0	38.0
12-13	34.2929534146495	38.0	34.0	38.0	24.0	38.0
14-15	34.15874498771859	37.5	34.0	38.0	24.0	38.0
16-17	34.13358910839124	37.5	34.0	38.0	23.5	38.0
18-19	34.19118835681145	37.5	34.0	38.0	24.0	38.0
20-21	34.100156404173575	37.0	34.0	38.0	23.5	38.0
22-23	34.168483509331764	37.5	34.0	38.0	23.5	38.0
24-25	34.1596319778305	37.0	34.0	38.0	24.0	38.0
26-27	34.090155459450386	37.0	34.0	38.0	23.5	38.0
28-29	34.15206631956836	37.0	34.0	38.0	24.0	38.0
30-31	34.20672118069406	37.5	34.0	38.0	24.0	38.0
32-33	34.172784624105134	37.5	34.0	38.0	24.0	38.0
34-35	34.20015010601894	37.5	34.0	38.0	24.0	38.0
36-37	34.049597967795435	37.0	34.0	38.0	23.0	38.0
38-39	34.063217727205924	37.0	34.0	38.0	23.5	38.0
40-41	34.03447977242668	37.0	34.0	38.0	23.0	38.0
42-43	34.11797755757563	37.0	34.0	38.0	24.0	38.0
44-45	34.15917273738795	37.0	34.0	38.0	24.0	38.0
46-47	34.14029664308357	37.0	34.0	38.0	24.0	38.0
48-49	34.08507232380912	37.0	34.0	38.0	24.0	38.0
50-51	33.977253164822706	37.0	34.0	38.0	23.0	38.0
52-53	34.06885719984045	37.0	34.0	38.0	23.5	38.0
54-55	34.05277066319569	37.0	34.0	38.0	24.0	38.0
56-57	34.00376577162891	37.0	34.0	38.0	23.5	38.0
58-59	34.00267671572229	37.0	34.0	38.0	23.5	38.0
60-61	33.97571536539794	37.0	34.0	38.0	23.0	38.0
62-63	33.94718472487561	37.0	34.0	38.0	23.0	38.0
64-65	33.97027008586484	37.0	34.0	38.0	23.0	38.0
66-67	33.86453456637205	37.0	34.0	38.0	23.0	38.0
68-69	33.62900457665904	37.0	34.0	38.0	22.5	38.0
70-71	33.77140322885394	37.0	34.0	38.0	23.0	38.0
72-73	33.74034282955094	37.0	34.0	38.0	22.5	38.0
74-75	33.75068492431717	37.0	34.0	38.0	22.5	38.0
76	32.342451661663134	37.0	31.0	38.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	31.0
14	114.0
15	379.0
16	883.0
17	1541.0
18	1982.0
19	1945.0
20	1782.0
21	1556.0
22	1383.0
23	1630.0
24	1920.0
25	2178.0
26	2660.0
27	3070.0
28	3851.0
29	4625.0
30	5781.0
31	6937.0
32	8583.0
33	10930.0
34	14098.0
35	18794.0
36	29368.0
37	64504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09058845758192	18.81153821930174	10.0738983477841	26.023974975332226
2	23.169336384439358	19.84915919635547	31.171141855436357	25.81036256376882
3	19.944785887860768	21.522481905831597	28.421096829387345	30.111635376920294
4	14.584426762958453	15.844057691096507	36.15560640732266	33.41590913862238
5	14.578653454537822	33.37444628723784	35.166271282514224	16.880628975710117
6	31.091890076207672	35.288560451787625	18.02899250519598	15.590556966808725
7	30.177083114647406	27.47779900489157	22.38101736191296	19.964100518548065
8	26.71047383116747	32.72678605168686	19.321688745197658	21.241051371948018
9	24.881384754267	15.211617995927194	21.627338189910354	38.27965905989545
10-11	26.14154052862511	23.83221716037201	26.65037893897088	23.375863372031993
12-13	25.819022526399767	22.196271492452713	26.009804127390673	25.974901853756847
14-15	23.926164633762305	23.54014023891	23.29661159280331	29.237083534524384
16-17	24.42581823525707	25.747118594251884	24.793473012407365	25.03359015808368
18-19	23.854785547834485	25.444807171498752	25.996682971889236	24.703724308777527
20-21	23.64852098335188	25.87701803371612	25.690435202485673	24.784025780446328
22-23	24.223490017424893	25.287615728591522	26.23680011756555	24.25209413641803
24-25	23.790754308987466	25.500965717044906	26.001931434089816	24.706348539877816
26-27	23.008208594881697	25.735571977410622	26.30712951105326	24.949089916654422
28-29	23.725673377700335	25.398358281023658	27.105945457980813	23.770022883295194
30-31	24.071022190498184	25.348235467008166	26.113986102072097	24.466756240421557
32-33	23.23625427749669	26.08065836709844	26.039720361933956	24.643366993470913
34-35	24.333707723636973	25.140921210085445	26.329435475405706	24.195935590871876
36-37	24.403249847794598	24.732590850880694	25.932651733042217	24.931507568282495
38-39	23.358805869880126	25.367654777150296	26.742751873701003	24.53078747926857
40-41	23.842189238553104	24.438939390758506	27.272321709739046	24.446549660949344
42-43	23.184032078600968	24.543908634770013	26.969485440765855	25.302573845863165
44-45	22.591218273046	24.456784162240464	27.58932882665379	25.362668738059746
46-47	22.963596666176812	24.052390149686143	27.953047257153653	25.030965926983395
48-49	22.220939264795415	24.071022190498184	28.52617932945647	25.18185921524993
50-51	22.06742174542859	24.336856800957317	27.96485629710495	25.63086515650914
52-53	21.516333214368192	24.4853882812336	28.42488400898537	25.573394495412842
54-55	20.99516091785107	24.99343942224928	28.426983393865594	25.584416266034054
56-57	20.863686939726662	25.09316020406021	28.40493985262318	25.63821300358995
58-59	21.43419478092919	24.75410954590305	27.674353914303108	26.13734175886465
60-61	21.135032435496402	24.307202989524068	27.997396762748515	26.560367812231018
62-63	20.883631096088845	24.05947557365692	28.336709844015708	26.72018348623853
64-65	21.66040350177398	24.365198496840428	27.362332836478913	26.612065164906685
66-67	21.612117649528688	24.16050847101799	27.09912245712006	27.128251422333253
68-69	21.46752251590284	24.545483173430185	26.665599479352547	27.32139483131443
70-71	22.04432851174606	24.532362017928747	26.726481640879225	26.696827829445972
72-73	21.927025381563205	24.63575672328008	26.250708542397078	27.186509352759643
74-75	21.93017445888355	25.020993848802302	26.192188188860666	26.856643503453487
76	22.462893372241933	25.00839753952092	25.830306720131002	26.698402368106144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	13.0
16	21.0
17	19.0
18	24.0
19	39.0
20	66.5
21	84.0
22	105.5
23	167.0
24	304.0
25	401.0
26	489.5
27	637.0
28	883.0
29	1070.0
30	1246.5
31	1564.0
32	2198.5
33	2692.0
34	3055.0
35	3874.5
36	4994.0
37	5657.0
38	6235.5
39	7738.0
40	9563.0
41	10809.5
42	11155.0
43	11473.0
44	11913.5
45	12183.0
46	12330.0
47	11887.5
48	10882.5
49	9895.0
50	9470.0
51	8698.0
52	7204.0
53	5943.5
54	5405.0
55	5032.5
56	4287.0
57	3577.5
58	3241.0
59	2932.5
60	2550.5
61	2289.5
62	2102.0
63	2069.5
64	2085.5
65	2085.0
66	2000.0
67	1964.0
68	1992.5
69	2001.5
70	1927.5
71	1873.0
72	1859.5
73	1756.5
74	1597.5
75	1528.0
76	1464.5
77	1246.5
78	991.0
79	890.0
80	801.0
81	595.5
82	434.0
83	389.0
84	330.5
85	214.0
86	121.0
87	86.0
88	77.5
89	50.0
90	21.5
91	9.0
92	6.0
93	4.0
94	2.5
95	3.0
96	3.0
97	2.5
98	2.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.248462200575232E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	190532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.34007935674847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.06031175857558	33.16660718409506
2	13.530233486330074	12.810446539164024
3	6.0611100024390785	8.608002855163436
4	2.8060489146100798	5.313543131862365
5	1.6075744473269915	3.8051350954170426
6	0.9955874853100957	2.8278714336699347
7	0.7838311270759883	2.597463943064682
8	0.5942482094946673	2.250540591606659
9	0.48448967826337613	2.064220183486239
>10	3.0200226169094657	24.88558352402746
>50	0.056542273664604535	1.6705855184430962
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0020993848802300925	0.0
2	0.0	0.0	0.0	0.0020993848802300925	0.0
3	0.0	0.0	0.0	0.0020993848802300925	0.0
4	0.0	0.0	0.0	0.0026242311002876158	0.0
5	0.0	0.0	0.0	0.0026242311002876158	0.0
6	0.0	0.0	0.0	0.0026242311002876158	0.0
7	0.0	0.0	0.0	0.0026242311002876158	0.0
8	0.0	0.0	0.0	0.0026242311002876158	0.0
9	0.0	0.0	0.0	0.0026242311002876158	0.0
10	0.0	0.0	0.0	0.0026242311002876158	0.0
11	0.0	0.0	0.0	0.0026242311002876158	0.0
12	0.0	0.0	0.0	0.0026242311002876158	0.0
13	0.0	0.0	0.0	0.0026242311002876158	0.0
14	0.0	0.0	0.0	0.0026242311002876158	0.0
15	0.0	0.0	0.0	0.0026242311002876158	0.0
16	0.0	0.0	0.0	0.0026242311002876158	0.0
17	0.0	0.0	0.0	0.0026242311002876158	0.0
18	0.0	0.0	0.0	0.003149077320345139	0.0
19	0.0	0.0	0.0	0.003149077320345139	0.0
20	0.0	0.0	0.0	0.0036739235404026622	0.0
21	0.0	0.0	0.0	0.0036739235404026622	0.0
22	0.0	0.0	0.0	0.005773308420632755	0.0
23	0.0	0.0	0.0	0.005773308420632755	0.0
24	0.0	0.0	0.0	0.007872693300862847	0.0
25	0.0	0.0	0.0	0.009447231961035417	0.0
26	0.0	0.0	0.0	0.010496924401150463	0.0
27	0.0	0.0	0.0	0.020469002582243403	0.0
28	0.0	0.0	0.0	0.04618646736506204	0.0
29	0.0	0.0	0.0	0.06560577750719039	0.0
30	0.0	0.0	0.0	0.08345054898914618	0.0
31	0.0	0.0	0.0	0.10601893645161968	0.0
32	0.0	0.0	0.0	0.1453824029559339	0.0
33	0.0	0.0	0.0	0.19419310142128357	0.0
34	0.0	0.0	0.0	0.2734448806499696	0.0
35	0.0	0.0	0.0	0.4104297440849831	0.0
36	0.0	0.0	0.0	0.6235173094283375	0.0
37	0.0	0.0	0.0	0.9389498876829089	0.0
38	0.0	0.0	0.0	1.2874477778011042	0.0
39	0.0	0.0	0.0	1.7671572229336805	0.0
40	0.0	0.0	0.0	2.2605126697877522	0.0
41	0.0	0.0	0.0	2.8278714336699347	0.0
42	0.0	0.0	0.0	3.4450905884575818	0.0
43	0.0	0.0	0.0	4.182499527638402	0.0
44	0.0	0.0	0.0	5.063191484894926	0.0
45	0.0	0.0	0.0	6.018411605399618	0.0
46	0.0	0.0	0.0	7.012470346188567	0.0
47	0.0	0.0	0.0	7.9787122373144665	0.0
48	0.0	0.0	0.0	8.900342199735478	0.0
49	0.0	0.0	0.0	9.735372535846997	0.0
50	0.0	0.0	0.0	10.504272248231269	0.0
51	0.0	0.0	0.0	11.259001112673987	0.0
52	0.0	0.0	0.0	12.003233052715554	0.0
53	0.0	0.0	0.0	12.842462158587534	0.0
54	0.0	0.0	0.0	13.651250183696177	0.0
55	0.0	0.0	0.0	14.573929838557303	0.0
56	0.0	0.0	0.0	15.469842336195494	0.0
57	0.0	0.0	0.0	16.29070182436546	0.0
58	0.0	0.0	0.0	17.066949383830536	0.0
59	0.0	0.0	0.0	17.781265089328826	0.0
60	0.0	0.0	0.0	18.51395041252913	0.0
61	0.0	0.0	0.0	19.24663573572943	0.0
62	0.0	0.0	0.0	19.992967060651228	0.0
63	0.0	0.0	0.0	20.74927046375412	0.0
64	0.0	0.0	0.0	21.51449625259799	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAACTA	15	0.0022220486	70.0	1
CGAGAAT	30	8.353398E-6	58.333332	14
TCGTAAA	20	0.006943182	52.5	2
CGTAAAC	20	0.006943182	52.5	3
GTTTCAC	30	5.866993E-4	46.666664	43
CGTGCGA	40	4.5803146E-5	43.75	10
CCGAAGC	45	9.1730224E-5	38.888885	3
GATGTAT	50	1.7051422E-4	35.000004	24
TGCGATG	50	1.7051422E-4	35.000004	29
TGTGCGC	40	0.0024143874	35.0	4
ATCGGGC	55	2.9841546E-4	31.818184	44
ACAAGGT	55	2.9841546E-4	31.818184	5
CTTGTTG	45	0.0042995317	31.111109	13
CAACGGC	45	0.0042995317	31.111109	48
AGGCGAG	45	0.0042995317	31.111109	45
ATACTGG	45	0.0042995317	31.111109	6
TAGGGAT	45	0.0042995317	31.111109	5
AGCCTTA	45	0.0042995317	31.111109	6
TACACCG	45	0.0042995317	31.111109	5
CAGATAA	60	4.968884E-4	29.166666	14
>>END_MODULE