FastQCFastQC Report
Wed 25 May 2016
SRR1781171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171821
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT52423.0508494305119864No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG5110.2974025293765023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC3530.20544636569453092No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT3160.18391232736394272No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT1890.10999819579678852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7700.059.54545241
GCCGTCT7300.059.45205346
TATGCCG7500.059.2666743
TCGTATG7750.059.16129340
CCGTCTT7350.059.04762347
CTCGTAT7600.058.94737239
GTCTTCT7600.058.48684749
CGTCTTC7450.057.78523348
CTTGAAA7650.057.6470657
TCTCGTA7200.057.36110738
GTATGCC7750.057.35484342
CTGCTTG7650.057.18954554
ATCTCGT7250.056.9655237
TTATCTC7200.056.87499635
TCTTCTG7750.056.45161450
ATGCCGT7850.056.1783544
TTGAAAA7950.055.471758
CTTCTGC7850.055.2866351
AATTATC7450.054.96644233
ATTATCT7450.054.96644234