##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781171_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171821
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.33283475244586	34.0	34.0	34.0	33.0	34.0
2	33.31758632530366	34.0	34.0	34.0	33.0	34.0
3	33.386023827122415	34.0	34.0	34.0	33.0	34.0
4	33.454181968443905	34.0	34.0	34.0	34.0	34.0
5	33.44761117674789	34.0	34.0	34.0	34.0	34.0
6	36.876563400282855	38.0	37.0	38.0	34.0	38.0
7	36.76670488473469	38.0	37.0	38.0	34.0	38.0
8	36.59115591225753	38.0	37.0	38.0	34.0	38.0
9	36.54381012798203	38.0	37.0	38.0	34.0	38.0
10-11	36.479824934088384	38.0	37.0	38.0	34.0	38.0
12-13	36.42205842126399	38.0	37.0	38.0	34.0	38.0
14-15	36.4154265194592	38.0	37.0	38.0	34.0	38.0
16-17	36.35381588979229	38.0	37.0	38.0	34.0	38.0
18-19	36.34663399700851	38.0	37.0	38.0	34.0	38.0
20-21	36.28710984105552	38.0	37.0	38.0	34.0	38.0
22-23	36.36038668148829	38.0	37.0	38.0	34.0	38.0
24-25	36.34940432193969	38.0	37.0	38.0	34.0	38.0
26-27	36.142264333230514	38.0	37.0	38.0	33.0	38.0
28-29	36.13040024211243	38.0	37.0	38.0	33.5	38.0
30-31	36.17617462359083	38.0	37.0	38.0	34.0	38.0
32-33	36.11607428661223	38.0	37.0	38.0	33.5	38.0
34-35	36.08088941398316	38.0	37.0	38.0	32.5	38.0
36-37	36.008479755093965	38.0	37.0	38.0	32.0	38.0
38-39	35.78362656485528	38.0	36.0	38.0	31.0	38.0
40-41	35.963776255521736	38.0	37.0	38.0	32.0	38.0
42-43	36.028666461026305	38.0	37.0	38.0	32.5	38.0
44-45	36.00635836131788	38.0	37.0	38.0	32.0	38.0
46-47	36.00547371974322	38.0	37.0	38.0	32.0	38.0
48-49	35.98836288928594	38.0	37.0	38.0	32.0	38.0
50-51	35.875562358501	38.0	36.5	38.0	31.5	38.0
52-53	35.90086194353426	38.0	37.0	38.0	32.0	38.0
54-55	35.92610914847428	38.0	37.0	38.0	32.0	38.0
56-57	35.88604710716385	38.0	37.0	38.0	32.0	38.0
58-59	35.84913660146315	38.0	37.0	38.0	31.5	38.0
60-61	35.84575808544939	38.0	36.5	38.0	31.5	38.0
62-63	35.804034431181286	38.0	36.0	38.0	31.0	38.0
64-65	35.76131555514169	38.0	36.0	38.0	31.0	38.0
66-67	35.68971778769766	38.0	36.0	38.0	31.0	38.0
68-69	35.56231485092043	38.0	36.0	38.0	31.0	38.0
70-71	35.27106698249923	38.0	36.0	38.0	29.0	38.0
72-73	35.04551248101221	38.0	36.0	38.0	27.0	38.0
74-75	35.05758609250324	38.0	36.0	38.0	27.0	38.0
76	34.17226648663435	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	11.0
22	18.0
23	42.0
24	126.0
25	264.0
26	502.0
27	880.0
28	1429.0
29	2375.0
30	3514.0
31	5143.0
32	7225.0
33	10175.0
34	14066.0
35	19718.0
36	28942.0
37	77389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64101012099802	18.48493490318413	10.224012198741713	29.65004277707614
2	22.16841945978664	18.83355352372527	33.86489427951182	25.133132736976272
3	19.641952962676275	21.305893924491183	30.142997654535826	28.909155458296716
4	14.229343328231126	18.998841817938438	34.754773863497476	32.017040990332966
5	14.190931259857642	33.21130711612666	37.1660041554874	15.431757468528293
6	30.871662951560054	38.73798895362034	16.056826581151316	14.333521513668293
7	29.225764021859955	27.242304491301994	20.932831260439645	22.599100226398402
8	25.553337484940723	35.930997957176366	17.80690369628858	20.70876086159433
9	23.924898586319486	15.07382683141176	19.527880759627752	41.473393822641
10-11	25.48029635492751	25.09442966808481	26.801438706560898	22.62383527042678
12-13	25.060382607481042	21.55353536529295	26.39432898190559	26.991753045320422
14-15	22.884280734019708	22.482118018170073	22.154742435441534	32.478858812368685
16-17	25.47331234249597	26.815988732459946	23.48170479743454	24.228994127609546
18-19	25.326939081951565	25.006838512172553	24.0055057298002	25.660716676075683
20-21	22.975945897183696	24.899459321037593	24.00492372876424	28.11967105301447
22-23	23.44387473009702	26.508983185990072	24.307564267464397	25.73957781644851
24-25	25.06678461887662	26.639933419081487	24.348886341017685	23.944395621024206
26-27	24.518830643518548	27.2254264612591	24.666949907170835	23.58879298805152
28-29	23.250068385121725	24.75366806152915	25.172999807939654	26.823263745409463
30-31	22.996606933960344	28.599821907682994	24.42600147828263	23.977569680074033
32-33	24.65909289318535	27.575500084390146	24.38438840421136	23.38101861821314
34-35	23.633898068338564	26.65099143876476	26.614325373499163	23.100785119397514
36-37	23.43630871662952	26.13213751520478	26.652155440836683	23.779398327329023
38-39	22.556032149737227	25.147100761839358	27.068577182067383	25.228289906356032
40-41	22.89853975940077	23.68162215328743	27.521082987527716	25.898755099784076
42-43	24.670732913904587	23.76252029728613	27.31971062908492	24.247036159724363
44-45	22.14106541109643	25.861507033482518	27.725074350632344	24.272353204788704
46-47	24.09426088778438	23.344352552947544	26.617817379714936	25.943569179553137
48-49	23.353955570040917	23.162186228691485	26.9722560106157	26.5116021906519
50-51	21.378353053468434	23.386547628054778	28.733100144918254	26.501999173558527
52-53	20.577519627984938	23.634771069892505	30.9746771349253	24.813032167197257
54-55	20.08194574586343	24.139656968589403	29.198700973687735	26.579696311859436
56-57	21.926889029862473	23.985717694577495	26.936753947422027	27.150639328138006
58-59	20.18641493181858	24.06283283184244	30.026015446307497	25.72473679003149
60-61	21.962391093056148	25.50212139377608	26.818898737639753	25.71658877552802
62-63	19.307302366998215	27.878722624126272	26.216236664901267	26.597738343974253
64-65	19.96612753970702	28.27593833117023	25.112762700717607	26.64517142840514
66-67	19.98155056716001	28.433951612433873	24.53221666734567	27.052281153060452
68-69	19.746713149149407	28.60971592529435	24.16351901106384	27.48005191449241
70-71	20.386041287153493	27.577246087498036	24.118704931294776	27.9180076940537
72-73	20.358687238463286	26.375413948236826	24.19873007373953	29.067168739560355
74-75	20.48061645549729	26.626547395254363	24.058758824590708	28.834077324657635
76	21.298909912059642	26.689403507138245	23.70606619679783	28.305620384004282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	8.5
16	12.5
17	10.0
18	16.5
19	37.0
20	60.5
21	70.0
22	93.5
23	148.0
24	240.0
25	301.0
26	406.5
27	550.5
28	725.5
29	862.0
30	1013.0
31	1277.0
32	1831.5
33	2273.0
34	2549.0
35	3197.5
36	4174.0
37	4778.0
38	5622.5
39	7721.5
40	9965.0
41	10942.5
42	10931.0
43	10932.5
44	10800.5
45	10489.5
46	10312.0
47	10224.5
48	9484.0
49	8575.5
50	8320.0
51	7676.5
52	6391.0
53	5234.5
54	4720.0
55	4344.5
56	3655.0
57	3064.0
58	2787.0
59	2438.5
60	2078.0
61	1983.5
62	1901.0
63	1852.5
64	1829.5
65	1865.0
66	1859.5
67	1844.0
68	1872.0
69	1896.5
70	1838.0
71	1783.0
72	1758.5
73	1680.5
74	1561.0
75	1495.0
76	1364.0
77	1166.0
78	968.5
79	838.0
80	752.5
81	561.5
82	399.5
83	343.0
84	268.0
85	154.5
86	95.5
87	75.0
88	59.0
89	31.0
90	12.0
91	3.5
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	171821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.44070864446138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.468405589840046	21.516578299509373
2	19.32660770079744	14.472037760227213
3	8.54797842408792	9.601271090262541
4	4.0633598109776	6.085402832017041
5	2.1793536553139234	4.079827262092526
6	1.4456482877617323	3.2475657806670895
7	1.0990035908038116	2.880323126975166
8	0.8596166700346644	2.574772583095198
9	0.6948438544403165	2.3413901676744984
>10	4.231241547620898	27.512352971988292
>50	0.06839626307689917	1.5236787121481077
>100	0.012435684195799846	0.8165474534544671
>500	0.0015544605244749807	0.2974025293765023
>1k	0.0	0.0
>5k	0.0015544605244749807	3.0508494305119864
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	5242	3.0508494305119864	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	511	0.2974025293765023	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	353	0.20544636569453092	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT	316	0.18391232736394272	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT	189	0.10999819579678852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.82001035961844E-4	0.0
2	0.0	0.0	0.0	5.82001035961844E-4	0.0
3	0.0	0.0	0.0	5.82001035961844E-4	0.0
4	0.0	0.0	0.0	0.001164002071923688	0.0
5	0.0	0.0	0.0	0.001164002071923688	0.0
6	0.0	0.0	0.0	0.001164002071923688	0.0
7	0.0	0.0	0.0	0.001164002071923688	0.0
8	0.0	0.0	0.0	0.001164002071923688	0.0
9	0.0	0.0	0.0	0.001164002071923688	0.0
10	0.0	0.0	0.0	0.001164002071923688	0.0
11	0.0	0.0	0.0	0.001164002071923688	0.0
12	0.0	0.0	0.0	0.001164002071923688	0.0
13	0.0	0.0	0.0	0.001164002071923688	0.0
14	0.0	0.0	0.0	0.001164002071923688	0.0
15	0.0	0.0	0.0	0.001164002071923688	0.0
16	0.0	0.0	0.0	0.001164002071923688	0.0
17	0.0	0.0	0.0	0.001164002071923688	0.0
18	0.0	0.0	0.0	0.001746003107885532	0.0
19	0.0	0.0	0.0	0.001746003107885532	0.0
20	0.0	0.0	0.0	0.001746003107885532	0.0
21	0.0	0.0	0.0	0.002328004143847376	0.0
22	0.0	0.0	0.0	0.0029100051798092203	0.0
23	0.0	0.0	0.0	0.004074007251732908	0.0
24	0.0	0.0	0.0	0.004656008287694752	0.0
25	0.0	0.0	0.0	0.0058200103596184405	0.0
26	0.0	0.0	0.0	0.006984012431542128	0.0
27	0.0	0.0	0.0	0.01746003107885532	0.0
28	0.0	0.0	0.0	0.042486075625214614	0.0
29	0.0	0.0	0.0	0.06169210981195546	0.0
30	0.0	0.0	0.0	0.08206214607062001	0.0
31	0.0	0.0	0.0	0.1018501812933227	0.0
32	0.0	0.0	0.0	0.1425902538106518	0.0
33	0.0	0.0	0.0	0.19497034704721775	0.0
34	0.0	0.0	0.0	0.2781964951897614	0.0
35	0.0	0.0	0.0	0.42486075625214614	0.0
36	0.0	0.0	0.0	0.6500951571693797	0.0
37	0.0	0.0	0.0	0.9864917559553256	0.0
38	0.0	0.0	0.0	1.3566444148270584	0.0
39	0.0	0.0	0.0	1.8699693285454049	0.0
40	0.0	0.0	0.0	2.3943522619470263	0.0
41	0.0	0.0	0.0	3.0019613434911916	0.0
42	0.0	0.0	0.0	3.6468184913369144	0.0
43	0.0	0.0	0.0	4.4191338660582815	0.0
44	0.0	0.0	0.0	5.342187509093766	0.0
45	0.0	0.0	0.0	6.332753272300825	0.0
46	0.0	0.0	0.0	7.375699128744449	0.0
47	0.0	0.0	0.0	8.379068914742668	0.0
48	0.0	0.0	0.0	9.328894605432398	0.0
49	0.0	0.0	0.0	10.192584142799774	0.0
50	0.0	0.0	0.0	10.993417568283272	0.0
51	0.0	0.0	0.0	11.777372963723876	0.0
52	0.0	0.0	0.0	12.554344346732938	0.0
53	0.0	0.0	0.0	13.409303868560887	0.0
54	0.0	0.0	0.0	14.260189383137101	0.0
55	0.0	0.0	0.0	15.21583508418645	0.0
56	0.0	0.0	0.0	16.13132271375443	0.0
57	0.0	0.0	0.0	17.007234272877007	0.0
58	0.0	0.0	0.0	17.808067698360503	0.0
59	0.0	0.0	0.0	18.56699704925475	0.0
60	0.0	0.0	0.0	19.31254037632187	0.0
61	0.0	0.0	0.0	20.062739711676688	0.0
62	0.0	0.0	0.0	20.836219088469978	0.0
63	0.0	0.0	0.0	21.61668247769481	0.0
64	0.0	0.0	0.0	22.412859894890612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	770	0.0	59.545452	41
GCCGTCT	730	0.0	59.452053	46
TATGCCG	750	0.0	59.26667	43
TCGTATG	775	0.0	59.161293	40
CCGTCTT	735	0.0	59.047623	47
CTCGTAT	760	0.0	58.947372	39
GTCTTCT	760	0.0	58.486847	49
CGTCTTC	745	0.0	57.785233	48
CTTGAAA	765	0.0	57.64706	57
TCTCGTA	720	0.0	57.361107	38
GTATGCC	775	0.0	57.354843	42
CTGCTTG	765	0.0	57.189545	54
ATCTCGT	725	0.0	56.96552	37
TTATCTC	720	0.0	56.874996	35
TCTTCTG	775	0.0	56.451614	50
ATGCCGT	785	0.0	56.17835	44
TTGAAAA	795	0.0	55.4717	58
CTTCTGC	785	0.0	55.28663	51
AATTATC	745	0.0	54.966442	33
ATTATCT	745	0.0	54.966442	34
>>END_MODULE