FastQCFastQC Report
Wed 25 May 2016
SRR1781170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255451
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGG252.3875061E-456.00000422
ATCGAAG305.870773E-446.66666451
CGGCTTT400.002415924335.026
GGCTAAA400.002415924335.01
TCTAGCA552.9869872E-431.8181843
GACCGTC450.00430225231.1111097
GTCTAGA450.00430225231.11110948
GAATTCA604.973576E-429.16666657
TGTCATA500.007200039528.00000214
ACCTAGA500.007200039528.00000240
GTCGTTA500.007200039528.00000234
GAACCTT500.007200039528.00000228
CTGCATA657.9423096E-426.92307713
ACGGTGT657.9423096E-426.92307770
CGGGAAT808.644792E-526.2513
TATAAGA808.644792E-526.252
TGGGGAC700.001223944525.043
ACCGTCC700.001223944525.08
GTATAAG1151.4902271E-624.3478281
TAGCGGC750.001829148623.33333438
Produced by FastQC (version 0.11.5)