##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781170_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255451
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205225268251056	34.0	32.0	34.0	23.0	34.0
2	31.37182081886546	34.0	33.0	34.0	23.0	34.0
3	31.378056848475833	34.0	33.0	34.0	23.0	34.0
4	31.566272983859918	34.0	33.0	34.0	23.0	34.0
5	31.5690523818658	34.0	33.0	34.0	23.0	34.0
6	34.339059154201784	37.0	34.0	38.0	25.0	38.0
7	34.23399790957953	37.0	34.0	38.0	24.0	38.0
8	33.956938904134255	37.0	34.0	38.0	23.0	38.0
9	34.291417923593954	37.0	34.0	38.0	24.0	38.0
10-11	34.17781100876489	37.0	34.0	38.0	24.0	38.0
12-13	34.16445815440143	37.0	34.0	38.0	24.0	38.0
14-15	34.049070076061554	37.0	34.0	38.0	24.0	38.0
16-17	34.0243275618416	37.0	34.0	38.0	23.5	38.0
18-19	34.066846479363946	37.0	34.0	38.0	23.5	38.0
20-21	33.98903703645709	37.0	34.0	38.0	23.5	38.0
22-23	34.06873529561442	37.0	34.0	38.0	23.5	38.0
24-25	34.05709118382782	37.0	34.0	38.0	23.5	38.0
26-27	33.98891372513711	37.0	34.0	38.0	23.0	38.0
28-29	34.06145405576804	37.0	34.0	38.0	23.5	38.0
30-31	34.13679727227531	37.0	34.0	38.0	24.0	38.0
32-33	34.082974817088214	37.0	34.0	38.0	23.5	38.0
34-35	34.112197642600734	37.0	34.0	38.0	24.0	38.0
36-37	33.946637515609645	37.0	34.0	38.0	23.0	38.0
38-39	33.96833247863583	37.0	34.0	38.0	23.0	38.0
40-41	33.95484065437207	37.0	34.0	38.0	23.0	38.0
42-43	34.01864349718733	37.0	34.0	38.0	24.0	38.0
44-45	34.03719108557023	37.0	34.0	38.0	24.0	38.0
46-47	34.04819123824139	37.0	34.0	38.0	24.0	38.0
48-49	34.011865289233555	37.0	34.0	38.0	23.5	38.0
50-51	33.88549663144791	37.0	34.0	38.0	23.0	38.0
52-53	33.958518463423516	37.0	34.0	38.0	23.5	38.0
54-55	33.93171097392455	37.0	34.0	38.0	23.0	38.0
56-57	33.90498764929478	37.0	34.0	38.0	23.5	38.0
58-59	33.89638521673432	37.0	34.0	38.0	23.0	38.0
60-61	33.86238848154832	37.0	34.0	38.0	23.0	38.0
62-63	33.83980293676674	37.0	34.0	38.0	23.0	38.0
64-65	33.8620342061687	37.0	34.0	38.0	23.0	38.0
66-67	33.75855447815824	37.0	34.0	38.0	23.0	38.0
68-69	33.510739828773424	37.0	34.0	38.0	22.5	38.0
70-71	33.66619038484876	37.0	34.0	38.0	23.0	38.0
72-73	33.656049496772376	37.0	34.0	38.0	23.0	38.0
74-75	33.64335821742722	37.0	34.0	38.0	22.5	38.0
76	32.192710147934434	36.0	31.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	27.0
14	92.0
15	266.0
16	669.0
17	1214.0
18	1758.0
19	1945.0
20	2070.0
21	2125.0
22	2248.0
23	2661.0
24	3093.0
25	3658.0
26	4366.0
27	5347.0
28	6214.0
29	7495.0
30	8808.0
31	10638.0
32	12577.0
33	15528.0
34	19338.0
35	25412.0
36	38134.0
37	79766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.96947751232135	17.576756403380685	9.722803981976973	25.73096210232099
2	24.232435966193126	19.474184873028484	30.592168361055545	25.701210799722844
3	20.504910922251234	20.886588817424865	28.272349687415588	30.336150572908306
4	15.036934676317571	15.504734763222691	35.2365032824299	34.221827278029835
5	14.948072233030993	32.69237544578021	34.85091074217756	17.50864157901124
6	31.664389648112557	34.72799088670626	18.094663947293217	15.512955517887972
7	31.2459140891991	27.54618302531601	21.459692856947125	19.748210028537763
8	27.2752895858697	32.55418847450196	19.52898990413034	20.641532035498
9	25.41857342504042	16.274353985695885	21.01851235657719	37.2885602326865
10-11	26.780674180175456	23.346160320374555	26.415633526586312	23.45753197286368
12-13	26.475136131782612	21.833541461963353	25.829415426050396	25.861906980203642
14-15	24.548347824044534	22.92005120355763	23.147687814884264	29.383913157513575
16-17	24.873459097830892	25.727047457242293	24.517617860176706	24.88187558475011
18-19	24.40663767219545	25.210118574599434	25.66421740372909	24.719026349476025
20-21	24.065672085840337	25.440103972973287	25.79555374611961	24.698670195066764
22-23	24.68947077913181	25.202485016695963	25.77206587564738	24.335978328524842
24-25	24.41681574940008	25.114405502425125	25.73194076359067	24.736837984584128
26-27	23.52642972624887	25.735463944161502	26.00870617065504	24.729400158934588
28-29	24.276475723328545	25.253179670465176	26.560279662244422	23.910064943961856
30-31	24.50274220887763	25.147288521086235	25.775393323964284	24.57457594607185
32-33	23.952343110811857	25.554803073779315	25.811603790942293	24.681250024466532
34-35	24.610590680795926	24.99148564695382	26.187996915259674	24.209926756990576
36-37	24.665199979643845	24.84507792101029	25.73507247965363	24.75464961969223
38-39	23.841558655084537	25.193285600761005	26.588856571318964	24.376299172835495
40-41	24.156883316174138	24.2784330458679	27.043542597210426	24.52114104074754
42-43	23.716094280312074	24.525838614841984	26.693964791682163	25.064102313163776
44-45	23.109911489874772	24.407224868957258	27.270592011775253	25.212271629392717
46-47	23.20660322331876	24.087398366027145	27.593354498514394	25.112643912139703
48-49	22.446966345796255	24.025742706037555	28.26021428767161	25.267076660494574
50-51	22.218938269961754	24.25748969469683	28.043538682565345	25.480033352776072
52-53	22.090146446872396	24.11401795256233	28.409557997424162	25.38627760314111
54-55	21.391186568069806	24.509592837765364	28.49450579563204	25.60471479853279
56-57	21.180382930581597	24.911039690586453	28.20834524037878	25.700232138453167
58-59	21.715906377348297	24.76247890984964	27.651486977933143	25.87012773486892
60-61	21.38198715213485	24.252987852856318	27.965050048737332	26.3999749462715
62-63	20.996198879628576	24.068412337395433	28.266869184305403	26.66851959867059
64-65	21.801049907810107	24.309163009735723	27.47904686221624	26.410740220237933
66-67	21.924361227789284	24.15805770969775	26.812774269820828	27.104806792692145
68-69	21.709055748460564	24.005778016136166	26.795941295982402	27.48922493942087
70-71	22.498443928581217	23.71315829650305	27.014182759120143	26.774215015795594
72-73	22.916136558478925	23.168631166055327	26.657362860196283	27.257869415269464
74-75	22.872096801343506	23.658548997655128	26.727239274851144	26.742114926150222
76	23.4526386665153	23.248294193406956	26.456737299912703	26.842329840165043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	6.0
15	15.0
16	27.0
17	36.0
18	51.0
19	74.0
20	109.0
21	136.0
22	183.0
23	290.0
24	480.5
25	611.0
26	729.5
27	964.0
28	1282.5
29	1485.0
30	1696.5
31	2219.0
32	3163.0
33	3796.0
34	4212.0
35	5273.0
36	6702.5
37	7487.0
38	8284.0
39	10054.0
40	12080.5
41	13724.5
42	14315.0
43	14685.5
44	15139.0
45	15219.0
46	15216.0
47	15024.0
48	14284.5
49	13065.0
50	12393.0
51	11434.5
52	9559.5
53	7909.0
54	7175.0
55	6635.5
56	5570.0
57	4696.5
58	4349.0
59	4019.0
60	3556.5
61	3409.0
62	3394.0
63	3324.0
64	3251.0
65	3228.5
66	3199.0
67	3189.0
68	3131.5
69	3062.5
70	3022.5
71	2994.0
72	2922.5
73	2721.5
74	2476.0
75	2360.0
76	2170.5
77	1827.0
78	1486.5
79	1300.0
80	1161.0
81	890.5
82	635.5
83	512.0
84	424.0
85	280.5
86	175.0
87	125.0
88	97.5
89	58.0
90	34.5
91	17.0
92	11.0
93	7.0
94	4.0
95	5.5
96	6.0
97	3.5
98	1.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	255451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.50662446799106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55651246687015	38.62204177273054
2	13.52762458182672	14.205798077238804
3	4.971748735170551	7.831492313604291
4	2.1583882724962615	4.533187299203087
5	1.1931538029392905	3.1324239331744366
6	0.7721075343558261	2.4324456213196726
7	0.5618810800303851	2.0651735205377264
8	0.48816993618955107	2.0505724412854334
9	0.3640520671964737	1.7203630661172982
>10	2.3521275148226963	21.580888642313692
>50	0.05423400810206899	1.825613312475017
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001174393523611182	0.0
2	0.0	0.0	0.0	0.001174393523611182	0.0
3	0.0	0.0	0.0	0.001174393523611182	0.0
4	0.0	0.0	0.0	0.00195732253935197	0.0
5	0.0	0.0	0.0	0.00195732253935197	0.0
6	0.0	0.0	0.0	0.00195732253935197	0.0
7	0.0	0.0	0.0	0.00195732253935197	0.0
8	0.0	0.0	0.0	0.00195732253935197	0.0
9	0.0	0.0	0.0	0.0027402515550927573	0.0
10	0.0	0.0	0.0	0.0027402515550927573	0.0
11	0.0	0.0	0.0	0.0027402515550927573	0.0
12	0.0	0.0	3.914645078703939E-4	0.0035231805708335453	0.0
13	0.0	0.0	3.914645078703939E-4	0.0035231805708335453	0.0
14	0.0	0.0	3.914645078703939E-4	0.0035231805708335453	0.0
15	0.0	0.0	3.914645078703939E-4	0.0035231805708335453	0.0
16	0.0	0.0	3.914645078703939E-4	0.0035231805708335453	0.0
17	0.0	0.0	3.914645078703939E-4	0.00391464507870394	0.0
18	0.0	0.0	3.914645078703939E-4	0.004306109586574333	0.0
19	0.0	0.0	3.914645078703939E-4	0.005089038602315121	0.0
20	0.0	0.0	3.914645078703939E-4	0.005871967618055909	0.0
21	0.0	0.0	3.914645078703939E-4	0.006654896633796697	0.0
22	0.0	0.0	3.914645078703939E-4	0.010178077204630242	0.0
23	0.0	0.0	3.914645078703939E-4	0.01096100622037103	0.0
24	0.0	0.0	3.914645078703939E-4	0.012526864251852605	0.0
25	0.0	0.0	3.914645078703939E-4	0.012918328759723	0.0
26	0.0	0.0	3.914645078703939E-4	0.013701257775463788	0.0
27	0.0	0.0	3.914645078703939E-4	0.02701105104305718	0.0
28	0.0	0.0	3.914645078703939E-4	0.04775866996018806	0.0
29	0.0	0.0	3.914645078703939E-4	0.06772335986157815	0.0
30	0.0	0.0	3.914645078703939E-4	0.08964537230232021	0.0
31	0.0	0.0	3.914645078703939E-4	0.11469910080602543	0.0
32	0.0	0.0	3.914645078703939E-4	0.1428845453726938	0.0
33	0.0	0.0	3.914645078703939E-4	0.17850781558889964	0.0
34	0.0	0.0	3.914645078703939E-4	0.2568007171629784	0.0
35	0.0	0.0	3.914645078703939E-4	0.37267421149261504	0.0
36	0.0	0.0	3.914645078703939E-4	0.5946345874551284	0.0
37	0.0	0.0	3.914645078703939E-4	0.917201341940333	0.0
38	0.0	0.0	3.914645078703939E-4	1.3035768112084118	0.0
39	0.0	0.0	3.914645078703939E-4	1.7596329628774208	0.0
40	0.0	0.0	3.914645078703939E-4	2.2732343972033777	0.0
41	0.0	0.0	3.914645078703939E-4	2.8416408626311895	0.0
42	0.0	0.0	3.914645078703939E-4	3.4206168697715023	0.0
43	0.0	0.0	3.914645078703939E-4	4.157744538091454	0.0
44	0.0	0.0	3.914645078703939E-4	5.014660345819746	0.0
45	0.0	0.0	3.914645078703939E-4	5.967876422484156	0.0
46	0.0	0.0	3.914645078703939E-4	6.953192588793937	0.0
47	0.0	0.0	3.914645078703939E-4	7.949078296816219	0.0
48	0.0	0.0	3.914645078703939E-4	8.872151606374608	0.0
49	0.0	0.0	3.914645078703939E-4	9.786612696759848	0.0
50	0.0	0.0	3.914645078703939E-4	10.610254021319157	0.0
51	0.0	0.0	3.914645078703939E-4	11.422934339658095	0.0
52	0.0	0.0	3.914645078703939E-4	12.259885457484996	0.0
53	0.0	0.0	3.914645078703939E-4	13.087441427123009	0.0
54	0.0	0.0	3.914645078703939E-4	13.970585356878619	0.0
55	0.0	0.0	3.914645078703939E-4	14.924975827066639	0.0
56	0.0	0.0	3.914645078703939E-4	15.81086000837734	0.0
57	0.0	0.0	3.914645078703939E-4	16.68343439642045	0.0
58	0.0	0.0	3.914645078703939E-4	17.478107347397348	0.0
59	0.0	0.0	3.914645078703939E-4	18.211320370638596	0.0
60	0.0	0.0	3.914645078703939E-4	18.995032315395125	0.0
61	0.0	0.0	3.914645078703939E-4	19.740772202888227	0.0
62	0.0	0.0	3.914645078703939E-4	20.53818540542022	0.0
63	0.0	0.0	3.914645078703939E-4	21.298018015196654	0.0
64	0.0	0.0	3.914645078703939E-4	22.08916778560272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGG	25	2.3875061E-4	56.000004	22
ATCGAAG	30	5.870773E-4	46.666664	51
CGGCTTT	40	0.0024159243	35.0	26
GGCTAAA	40	0.0024159243	35.0	1
TCTAGCA	55	2.9869872E-4	31.818184	3
GACCGTC	45	0.004302252	31.111109	7
GTCTAGA	45	0.004302252	31.111109	48
GAATTCA	60	4.973576E-4	29.166666	57
TGTCATA	50	0.0072000395	28.000002	14
ACCTAGA	50	0.0072000395	28.000002	40
GTCGTTA	50	0.0072000395	28.000002	34
GAACCTT	50	0.0072000395	28.000002	28
CTGCATA	65	7.9423096E-4	26.923077	13
ACGGTGT	65	7.9423096E-4	26.923077	70
CGGGAAT	80	8.644792E-5	26.25	13
TATAAGA	80	8.644792E-5	26.25	2
TGGGGAC	70	0.0012239445	25.0	43
ACCGTCC	70	0.0012239445	25.0	8
GTATAAG	115	1.4902271E-6	24.347828	1
TAGCGGC	75	0.0018291486	23.333334	38
>>END_MODULE