FastQCFastQC Report
Wed 25 May 2016
SRR1781170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230973
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT36301.5716122663687964No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3570.15456352041147667No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT2730.1181956332558351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT252.8338054E-670.0000139
TCGCTAC150.002222647470.0000113
ATATCGT207.9152305E-570.037
CCGTCTT5700.058.9473747
CGTATGC5900.056.9491541
CGTCTTC5900.056.9491548
TATGCCG5900.056.9491543
TCGTATG5900.056.9491540
ATCTCGT4800.056.87500437
CTCGTAT5550.056.7567639
TCTCGTA4800.056.14583638
TCTTCTG5950.055.29411750
GCCGTCT6250.054.8846
GTATGCC6150.054.63414842
AGATCTC5000.054.635
CTTGAAA6200.054.19354657
ATGCCGT6300.053.8888944
ACAGATC5150.053.6893233
CAGATCT5200.053.17307734
GATCTCG5150.053.0097136
Produced by FastQC (version 0.11.5)