##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781170_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230973
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25028033579682	34.0	34.0	34.0	32.0	34.0
2	33.282167179713646	34.0	34.0	34.0	33.0	34.0
3	33.314867971581094	34.0	34.0	34.0	33.0	34.0
4	33.3850363462396	34.0	34.0	34.0	33.0	34.0
5	33.37443337532958	34.0	34.0	34.0	33.0	34.0
6	36.75573335411498	38.0	37.0	38.0	34.0	38.0
7	36.67183177254484	38.0	37.0	38.0	34.0	38.0
8	36.4763846856559	38.0	37.0	38.0	34.0	38.0
9	36.42449550380348	38.0	37.0	38.0	34.0	38.0
10-11	36.343812913197645	38.0	37.0	38.0	34.0	38.0
12-13	36.26950985613037	38.0	37.0	38.0	34.0	38.0
14-15	36.2890619249869	38.0	37.0	38.0	34.0	38.0
16-17	36.21929835954852	38.0	37.0	38.0	34.0	38.0
18-19	36.2209565620224	38.0	37.0	38.0	34.0	38.0
20-21	36.17286219601425	38.0	37.0	38.0	33.5	38.0
22-23	36.22352179691998	38.0	37.0	38.0	34.0	38.0
24-25	36.21887190277651	38.0	37.0	38.0	34.0	38.0
26-27	35.99489550726708	38.0	36.5	38.0	32.0	38.0
28-29	35.98420378139436	38.0	37.0	38.0	32.0	38.0
30-31	36.01284349252943	38.0	37.0	38.0	32.0	38.0
32-33	35.960876379490244	38.0	37.0	38.0	32.0	38.0
34-35	35.97225866226788	38.0	37.0	38.0	32.0	38.0
36-37	35.90265095920302	38.0	36.0	38.0	31.5	38.0
38-39	35.751349724859615	38.0	36.0	38.0	31.0	38.0
40-41	35.85888177406017	38.0	36.0	38.0	31.5	38.0
42-43	35.890803254059996	38.0	36.0	38.0	31.5	38.0
44-45	35.85813493352037	38.0	36.0	38.0	31.0	38.0
46-47	35.882815307416884	38.0	36.0	38.0	31.5	38.0
48-49	35.84516155567967	38.0	36.0	38.0	31.0	38.0
50-51	35.7653405376386	38.0	36.0	38.0	31.0	38.0
52-53	35.79838985509129	38.0	36.0	38.0	31.0	38.0
54-55	35.81133509111454	38.0	36.0	38.0	31.0	38.0
56-57	35.759424261710244	38.0	36.0	38.0	31.0	38.0
58-59	35.73583275967321	38.0	36.0	38.0	31.0	38.0
60-61	35.73711212998922	38.0	36.0	38.0	31.0	38.0
62-63	35.70033726885826	38.0	36.0	38.0	31.0	38.0
64-65	35.663746411918275	38.0	36.0	38.0	31.0	38.0
66-67	35.60574179666022	38.0	36.0	38.0	31.0	38.0
68-69	35.532003740696965	38.0	36.0	38.0	31.0	38.0
70-71	35.39197438661662	38.0	36.0	38.0	30.0	38.0
72-73	35.28117572183762	38.0	36.0	38.0	28.5	38.0
74-75	35.29890723158118	38.0	36.0	38.0	29.0	38.0
76	34.378745567663756	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	12.0
22	18.0
23	78.0
24	191.0
25	327.0
26	726.0
27	1283.0
28	2100.0
29	3406.0
30	5067.0
31	7514.0
32	10487.0
33	14151.0
34	19391.0
35	26636.0
36	39438.0
37	100143.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.09248267113472	18.164460781130263	10.274361072506311	28.468695475228706
2	23.17716789408286	19.461582089681478	31.498053885086136	25.86319613114953
3	20.476419321738902	21.35747468318808	28.43882185363657	29.727284141436446
4	14.836366155351492	17.13187255653258	34.4360596260169	33.59570166209903
5	14.629848510431955	33.16621423283241	35.42838340412083	16.77555385261481
6	31.551306862706895	36.65320188939833	16.739618916496735	15.055872331398042
7	30.151143207214698	27.470310382598832	20.518848523420484	21.859697886765986
8	26.285756343815077	34.52005212730492	18.209920640074813	20.984270888805185
9	24.402419330397926	15.656375420503696	19.71615729977097	40.22504794932741
10-11	26.058024098054748	24.554601620102783	26.03118113372559	23.35619314811688
12-13	25.643040528546628	21.990882051148837	25.71707515597061	26.649002264333927
14-15	23.53327012248185	22.882544713018405	22.256281037177505	31.327904127322242
16-17	25.295380845380194	26.48361496798327	23.541496192195623	24.679507994440907
18-19	24.90810614227637	25.210738917535814	24.534252921337128	25.346902018850688
20-21	23.606005896793132	25.277846328358727	24.44138492377897	26.674762851069172
22-23	23.953665579959562	26.079022223376757	24.730812692392618	25.23649950427106
24-25	24.886242114879227	25.969052659834702	24.649634372848777	24.495070852437298
26-27	24.094591142687673	26.337710468323138	25.177834638680714	24.38986375030848
28-29	23.64756919639958	26.154788654951012	25.43479107947682	24.762851069172587
30-31	23.742168998108003	27.2215800115165	24.883644408653826	24.152606581721674
32-33	23.197949543886082	26.711347213743597	25.772276413260425	24.318426829109896
34-35	23.894351287812864	25.986803652374952	25.08345131249107	25.035393747321116
36-37	24.88451031072896	25.748897057231794	24.63794469483446	24.72864793720478
38-39	23.119585406086426	25.41985426868075	26.23639992553242	25.224160399700395
40-41	23.290817541444238	24.264091473895217	26.901196243716797	25.543894740943745
42-43	24.19784996514744	24.28725435440506	26.70766712992428	24.807228550523224
44-45	22.3842180687786	25.35902464790257	27.26184445801024	24.994912825308585
46-47	23.6235404138146	23.659042398895107	26.858117615478864	25.859299571811423
48-49	22.926056292293904	23.677442817991714	27.148844237205218	26.247656652509164
50-51	21.599277837669337	23.80602927614916	28.286856039450498	26.307836846731004
52-53	21.0723764249501	23.92400843388621	29.709100197858625	25.294514943305064
54-55	20.40346707190884	24.232486048152815	28.626722603940717	26.737324275997626
56-57	21.414840695665728	24.33877552787555	27.408181908707945	26.838201867750776
58-59	20.572750927597596	24.26495737597035	28.842115745130382	26.320175951301668
60-61	21.415273646703294	25.16073307269681	26.784732414611234	26.639260865988668
62-63	19.627185861550917	26.22903975789378	26.772393310040567	27.371381070514733
64-65	20.155602602901638	26.53383728834106	25.87856589298316	27.431994215774136
66-67	20.529672299359667	26.598563468457353	24.929970169673513	27.941794062509473
68-69	20.25561429257965	26.945140774029863	24.607421646685975	28.19182328670451
70-71	20.561494200620853	26.832140553224836	24.314097318734223	28.292267927420088
72-73	20.331813675191473	26.10434985907444	24.394193260684148	29.16964320504994
74-75	20.45368939226663	26.64813636225879	24.181614301238675	28.716559944235907
76	20.917163477982275	27.24647469617661	23.610984833725155	28.225376992115965
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.5
14	2.5
15	11.0
16	22.0
17	26.0
18	34.0
19	53.0
20	104.0
21	144.0
22	177.0
23	267.0
24	428.5
25	533.0
26	684.0
27	909.5
28	1204.0
29	1424.0
30	1577.5
31	2016.0
32	2896.0
33	3491.0
34	3756.5
35	4549.5
36	5695.5
37	6314.0
38	7295.0
39	9623.5
40	11958.0
41	13255.0
42	13565.0
43	13608.0
44	13705.5
45	13618.0
46	13476.0
47	13480.0
48	12718.5
49	11396.5
50	10840.0
51	10092.0
52	8278.5
53	6897.0
54	6581.0
55	5944.0
56	4945.0
57	4134.0
58	3685.0
59	3461.5
60	3147.5
61	2952.5
62	2848.0
63	2798.0
64	2795.5
65	2869.5
66	2839.5
67	2783.0
68	2810.5
69	2847.0
70	2792.5
71	2729.0
72	2701.5
73	2503.0
74	2332.0
75	2332.0
76	2108.0
77	1706.5
78	1385.0
79	1241.0
80	1125.5
81	875.5
82	618.5
83	496.0
84	397.0
85	232.0
86	145.0
87	124.0
88	98.5
89	51.0
90	22.5
91	9.0
92	2.0
93	4.5
94	3.5
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	230973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.406734120672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.889999999926516	27.298495088458726
2	19.310999999977437	16.76454885206635
3	7.2059999999915805	9.383667782195909
4	2.9719999999965276	5.160192552259458
5	1.5470000001143323	3.3575108844821187
6	0.9869999999995457	2.5705467946250122
7	0.7269999999991555	2.208968699398432
8	0.6449999999992465	2.2397874806240585
9	0.4769999999994427	1.8634510957982717
>10	3.139999999996331	24.52089817208017
>50	0.08999999999989486	2.450310141109071
>100	0.006999999999991822	0.6059580083238731
>500	0.0	0.0
>1k	9.999999999988317E-4	1.5756644485785527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	3630	1.5716122663687964	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG	357	0.15456352041147667	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT	273	0.1181956332558351	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012988531127014846	0.0
2	0.0	0.0	0.0	0.0012988531127014846	0.0
3	0.0	0.0	0.0	0.0012988531127014846	0.0
4	0.0	0.0	0.0	0.0021647551878358076	0.0
5	0.0	0.0	0.0	0.0021647551878358076	0.0
6	0.0	0.0	0.0	0.0021647551878358076	0.0
7	0.0	0.0	0.0	0.0021647551878358076	0.0
8	0.0	0.0	0.0	0.0021647551878358076	0.0
9	0.0	0.0	0.0	0.003030657262970131	0.0
10	0.0	0.0	0.0	0.003030657262970131	0.0
11	0.0	0.0	0.0	0.003030657262970131	0.0
12	0.0	0.0	0.0	0.003463608300537292	0.0
13	0.0	0.0	0.0	0.003463608300537292	0.0
14	0.0	0.0	0.0	0.003463608300537292	0.0
15	0.0	0.0	0.0	0.003463608300537292	0.0
16	0.0	0.0	0.0	0.003463608300537292	0.0
17	0.0	0.0	0.0	0.0038965593381044537	0.0
18	0.0	0.0	0.0	0.004329510375671615	0.0
19	0.0	0.0	0.0	0.0047624614132387765	0.0
20	0.0	0.0	0.0	0.0051954124508059385	0.0
21	0.0	0.0	0.0	0.006061314525940262	0.0
22	0.0	0.0	0.0	0.008226069713776069	0.0
23	0.0	0.0	0.0	0.010390824901611877	0.0
24	0.0	0.0	0.0	0.010390824901611877	0.0
25	0.0	0.0	0.0	0.0112567269767462	0.0
26	0.0	0.0	0.0	0.012555580089447684	0.0
27	0.0	0.0	0.0	0.029007719516999823	0.0
28	0.0	0.0	0.0	0.054551830733462356	0.0
29	0.0	0.0	0.0	0.07619938261182042	0.0
30	0.0	0.0	0.0	0.09871283656531282	0.0
31	0.0	0.0	0.0	0.12728760504474548	0.0
32	0.0	0.0	0.0	0.1532646672987752	0.0
33	0.0	0.0	0.0	0.19266321171738687	0.0
34	0.0	0.0	0.0	0.28185112545622215	0.0
35	0.0	0.0	0.0	0.4121693877639378	0.0
36	0.0	0.0	0.0	0.6507254094634438	0.0
37	0.0	0.0	0.0	1.0066111623436507	0.0
38	0.0	0.0	0.0	1.4270066198213645	0.0
39	0.0	0.0	0.0	1.9114788308590183	0.0
40	0.0	0.0	0.0	2.4587289423439103	0.0
41	0.0	0.0	0.0	3.0700558073887425	0.0
42	0.0	0.0	0.0	3.694371203560589	0.0
43	0.0	0.0	0.0	4.476280777406883	0.0
44	0.0	0.0	0.0	5.392838123936564	0.0
45	0.0	0.0	0.0	6.4024799435431845	0.0
46	0.0	0.0	0.0	7.456282768981656	0.0
47	0.0	0.0	0.0	8.504457230931754	0.0
48	0.0	0.0	0.0	9.48422542894624	0.0
49	0.0	0.0	0.0	10.44234607508237	0.0
50	0.0	0.0	0.0	11.307382248141558	0.0
51	0.0	0.0	0.0	12.162893498374268	0.0
52	0.0	0.0	0.0	13.03961934944777	0.0
53	0.0	0.0	0.0	13.904222571469392	0.0
54	0.0	0.0	0.0	14.848488784403372	0.0
55	0.0	0.0	0.0	15.84341026873271	8.65902075134323E-4
56	0.0	0.0	0.0	16.768193684976165	8.65902075134323E-4
57	0.0	0.0	0.0	17.688647590843953	8.65902075134323E-4
58	0.0	0.0	0.0	18.5160170236348	8.65902075134323E-4
59	0.0	0.0	0.0	19.31178103068324	8.65902075134323E-4
60	0.0	0.0	0.0	20.11750291159573	8.65902075134323E-4
61	0.0	0.0	0.0	20.89075346469068	8.65902075134323E-4
62	0.0	0.0	0.0	21.722452407857194	8.65902075134323E-4
63	0.0	0.0	0.0	22.507825590004025	8.65902075134323E-4
64	0.0	0.0	0.0	23.32047468751759	8.65902075134323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	25	2.8338054E-6	70.00001	39
TCGCTAC	15	0.0022226474	70.00001	13
ATATCGT	20	7.9152305E-5	70.0	37
CCGTCTT	570	0.0	58.94737	47
CGTATGC	590	0.0	56.94915	41
CGTCTTC	590	0.0	56.94915	48
TATGCCG	590	0.0	56.94915	43
TCGTATG	590	0.0	56.94915	40
ATCTCGT	480	0.0	56.875004	37
CTCGTAT	555	0.0	56.75676	39
TCTCGTA	480	0.0	56.145836	38
TCTTCTG	595	0.0	55.294117	50
GCCGTCT	625	0.0	54.88	46
GTATGCC	615	0.0	54.634148	42
AGATCTC	500	0.0	54.6	35
CTTGAAA	620	0.0	54.193546	57
ATGCCGT	630	0.0	53.88889	44
ACAGATC	515	0.0	53.68932	33
CAGATCT	520	0.0	53.173077	34
GATCTCG	515	0.0	53.00971	36
>>END_MODULE