FastQCFastQC Report
Wed 25 May 2016
SRR1781169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276565
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGTC150.002223112970.0000161
GATAACT200.00694648352.5000043
ACGGAGC200.00694648352.5000043
ATAAGTT200.00694648352.50000465
GTACTAG200.00694648352.5000041
CTCGCTT350.001254115840.00000418
TCGTTCG459.1837835E-538.8888923
TTCGGTA459.1837835E-538.8888926
CGGTAAG459.1837835E-538.8888928
GAAATCG654.8522816E-737.6923148
CGTGCGA400.002416268235.00000410
TACGGAC400.002416268235.00000438
TAACACG400.002416268235.0000044
CGAGAAT400.002416268235.00000414
AATCCGA652.0982823E-532.30769320
ATCCGAG652.0982823E-532.30769321
CGTTCGG552.9876217E-431.8181824
CGTTCAT552.9876217E-431.8181842
CGCAAAA450.004302860231.11111358
TATCGGC450.004302860231.11111312