##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781169_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276565
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569363440782457	34.0	33.0	34.0	23.0	34.0
2	31.435347929058267	34.0	33.0	34.0	23.0	34.0
3	31.42548044763437	34.0	33.0	34.0	23.0	34.0
4	31.589789018856326	34.0	33.0	34.0	23.0	34.0
5	31.57689150832535	34.0	33.0	34.0	23.0	34.0
6	34.42855386617974	37.0	34.0	38.0	26.0	38.0
7	34.31251242926618	37.0	34.0	38.0	24.0	38.0
8	34.15812557626598	37.0	34.0	38.0	24.0	38.0
9	34.47577603818271	37.0	34.0	38.0	26.0	38.0
10-11	34.33910111547014	37.0	34.0	38.0	25.0	38.0
12-13	34.29768951241118	37.0	34.0	38.0	24.0	38.0
14-15	34.16581635420245	37.0	34.0	38.0	24.0	38.0
16-17	34.14190696581274	37.0	34.0	38.0	24.0	38.0
18-19	34.21549002946866	37.0	34.0	38.0	24.0	38.0
20-21	34.124343282772585	37.0	34.0	38.0	24.0	38.0
22-23	34.20340426301232	37.0	34.0	38.0	24.0	38.0
24-25	34.19058629978486	37.0	34.0	38.0	24.0	38.0
26-27	34.13231066837814	37.0	34.0	38.0	24.0	38.0
28-29	34.225894455191366	37.0	34.0	38.0	24.0	38.0
30-31	34.309664997378555	37.0	34.0	38.0	24.5	38.0
32-33	34.244649539891164	37.0	34.0	38.0	24.0	38.0
34-35	34.255608988845296	37.0	34.0	38.0	24.0	38.0
36-37	34.09392367074648	37.0	34.0	38.0	24.0	38.0
38-39	34.12362193336105	37.0	34.0	38.0	24.0	38.0
40-41	34.117353967421764	37.0	34.0	38.0	24.0	38.0
42-43	34.20759857538011	37.0	34.0	38.0	24.0	38.0
44-45	34.23052266194204	37.0	34.0	38.0	24.0	38.0
46-47	34.2155496899463	37.0	34.0	38.0	24.0	38.0
48-49	34.175362030625706	37.0	34.0	38.0	24.0	38.0
50-51	34.043280964691846	37.0	34.0	38.0	24.0	38.0
52-53	34.13078299857176	37.0	34.0	38.0	24.0	38.0
54-55	34.09678375788694	37.0	34.0	38.0	24.0	38.0
56-57	34.09155171478676	37.0	34.0	38.0	24.0	38.0
58-59	34.07365176359988	37.0	34.0	38.0	24.0	38.0
60-61	34.05486594471462	37.0	34.0	38.0	24.0	38.0
62-63	34.03291450472764	37.0	34.0	38.0	24.0	38.0
64-65	34.04704680635655	37.0	34.0	38.0	24.0	38.0
66-67	33.95841303129463	37.0	34.0	38.0	24.0	38.0
68-69	33.74583913365755	37.0	34.0	38.0	22.5	38.0
70-71	33.90318550792762	37.0	34.0	38.0	23.5	38.0
72-73	33.88330952940538	37.0	34.0	38.0	23.0	38.0
74-75	33.893882089201455	37.0	34.0	38.0	23.5	38.0
76	32.55781461862492	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	19.0
14	100.0
15	277.0
16	626.0
17	1169.0
18	1594.0
19	1888.0
20	1890.0
21	1979.0
22	2247.0
23	2482.0
24	3070.0
25	3721.0
26	4566.0
27	5661.0
28	6645.0
29	7857.0
30	9339.0
31	11525.0
32	13739.0
33	16789.0
34	21314.0
35	27660.0
36	41517.0
37	88886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.772133133259814	18.49402491276915	9.300887675591634	24.432954278379405
2	24.49623054254877	20.483430658253937	29.896046137436045	25.12429266176125
3	19.709290763473327	22.105472492904017	29.687776833655743	28.497459909966917
4	14.176052645851788	17.136658651673205	37.54054200639994	31.146746696075063
5	13.709254605608084	33.70636197638891	36.536799667347644	16.047583750655363
6	28.780575994793267	37.76255129897131	19.421474156165818	14.035398550069603
7	28.949433225462368	29.399237069043444	22.55672265109468	19.09460705439951
8	25.807676314790374	34.211125775134235	20.291432393831467	19.68976551624392
9	24.944588071520258	16.763871061052555	21.92721421727261	36.364326650154574
10-11	25.213241010250755	24.556433388172763	27.96141955778931	22.268906043787176
12-13	25.512628134434944	23.195451340552854	26.691916909225675	24.600003615786523
14-15	23.821343987850955	24.08059588161915	24.1550810840128	27.94297904651709
16-17	23.856236327807206	26.96237774121816	25.498164988339088	23.683220942635547
18-19	23.239021568166613	26.64690036700233	26.79135103863468	23.322727026196375
20-21	23.04304593856779	26.828774429157704	26.823169960045558	23.30500967222895
22-23	23.380218031927395	26.413320557554282	27.27460090756242	22.931860502955907
24-25	23.040334098674816	26.61923960009401	27.072659230199047	23.267767071032125
26-27	22.54876792074196	26.899824634353585	27.300815359861154	23.250592085043298
28-29	22.949758646249528	26.528664147668724	28.130819156437003	22.39075804964475
30-31	23.171587149494695	26.373185327138287	27.395187388136605	23.060040135230416
32-33	22.517491367309674	27.140816806175767	27.134850758411222	23.20684106810334
34-35	23.706542765715113	26.230180970115523	27.154014426988233	22.909261837181134
36-37	23.64995570661508	26.13002368340173	26.791170249308482	23.428850360674705
38-39	22.90654999728816	26.831486269050675	27.481966264711733	22.779997468949436
40-41	23.232513152423483	25.668830112270168	27.93719378807875	23.161462947227594
42-43	22.512429266176127	26.110498436172332	27.736879214651168	23.64019308300038
44-45	22.1076419648184	26.075063728237485	28.043497911883282	23.773796395060838
46-47	22.438486431761067	25.407047167935204	28.186863847558442	23.967602552745284
48-49	21.857610326686313	25.4672500135592	28.83897094715528	23.83616871259921
50-51	21.98723627357041	25.355522209968722	28.48588939309023	24.17135212337064
52-53	21.530020067615208	25.35588378862112	28.891942219731348	24.222153924032323
54-55	20.896534268616783	25.88342704246741	28.999692658145467	24.220346030770344
56-57	20.749914125070056	25.86896389637156	28.99788476488348	24.383237213674906
58-59	21.019471010431545	25.882703885162623	28.425867336792436	24.6719577676134
60-61	20.766185164427892	25.4674308028854	28.61533455064813	25.15104948203858
62-63	20.37387232657784	25.137851861226114	29.26021007719704	25.228065734999007
64-65	20.98891761430405	25.403069802758843	28.554950915697937	25.053061667239167
66-67	20.98819445699926	25.29224594579936	28.012944515755787	25.706615081445594
68-69	20.837416158949974	25.40180427747546	27.774844973152785	25.985934590421785
70-71	21.578110028383925	25.01780774863052	27.979498490409128	25.424583732576426
72-73	21.913112649829152	24.452841104261207	27.690777936470628	25.94326830943901
74-75	21.973677074105545	24.77030716106521	27.58248513007792	25.673530634751323
76	22.271798673006344	24.53672735161716	27.430802885397647	25.76067108997885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	5.0
15	21.0
16	39.5
17	47.0
18	57.5
19	92.0
20	136.5
21	157.0
22	199.5
23	310.0
24	487.0
25	596.0
26	769.0
27	1084.5
28	1426.5
29	1626.0
30	1987.0
31	2587.5
32	3606.5
33	4386.0
34	4892.0
35	6180.5
36	8026.5
37	9090.0
38	9980.0
39	12437.0
40	14821.0
41	16652.0
42	17666.0
43	18085.0
44	18790.0
45	19108.0
46	19140.0
47	18730.5
48	17421.5
49	15854.5
50	15187.0
51	13838.0
52	11307.5
53	9236.5
54	8347.0
55	7627.0
56	6275.0
57	4851.0
58	4059.0
59	3600.5
60	2884.5
61	2431.5
62	2236.0
63	2047.0
64	1804.0
65	1694.0
66	1602.0
67	1566.0
68	1566.0
69	1531.5
70	1472.5
71	1448.0
72	1406.5
73	1327.5
74	1242.0
75	1194.0
76	1101.5
77	957.5
78	815.0
79	724.0
80	649.0
81	483.0
82	348.5
83	305.0
84	251.0
85	162.5
86	104.0
87	80.0
88	63.0
89	37.0
90	23.0
91	12.0
92	6.0
93	6.5
94	4.0
95	1.0
96	1.0
97	1.5
98	3.5
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	276565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.547977740404946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3019270483854	32.654526144802595
2	11.97590017155515	10.67004268527501
3	4.747521521535565	6.344774491903793
4	2.22261036982037	3.9605118912140416
5	1.3001214931155782	2.895889166756741
6	0.9350823634939773	2.4993616988624967
7	0.7143683169816286	2.2276564718343694
8	0.5619027603358875	2.002530532777217
9	0.49297736494845434	1.9765030212222507
>10	3.600831027817872	30.378518402719727
>50	0.1410818551920719	4.0624366605471165
>100	0.005675706818071857	0.32724883208461697
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0018078932619818126	0.0
2	0.0	0.0	0.0	0.0018078932619818126	0.0
3	0.0	0.0	0.0	0.0018078932619818126	0.0
4	0.0	0.0	0.0	0.0036157865239636253	0.0
5	0.0	0.0	0.0	0.0036157865239636253	0.0
6	0.0	0.0	0.0	0.0036157865239636253	0.0
7	0.0	0.0	0.0	0.0036157865239636253	0.0
8	0.0	0.0	0.0	0.0036157865239636253	0.0
9	0.0	0.0	0.0	0.0039773651763599874	0.0
10	0.0	0.0	0.0	0.004700522481152713	0.0
11	0.0	0.0	0.0	0.005423679785945438	0.0
12	0.0	0.0	0.0	0.005423679785945438	0.0
13	0.0	0.0	0.0	0.005423679785945438	0.0
14	0.0	0.0	0.0	0.005423679785945438	0.0
15	0.0	0.0	0.0	0.0057852584383418	0.0
16	0.0	0.0	0.0	0.0057852584383418	0.0
17	0.0	0.0	0.0	0.0068699943955308875	0.0
18	0.0	0.0	0.0	0.0072315730479272505	0.0
19	0.0	0.0	0.0	0.0072315730479272505	0.0
20	0.0	0.0	0.0	0.0072315730479272505	0.0
21	0.0	0.0	0.0	0.0072315730479272505	0.0
22	0.0	0.0	0.0	0.008316309005116337	0.0
23	0.0	0.0	0.0	0.008677887657512701	0.0
24	0.0	0.0	0.0	0.009039466309909063	0.0
25	0.0	0.0	0.0	0.009401044962305425	0.0
26	0.0	0.0	0.0	0.011208938224287238	0.0
27	0.0	0.0	0.0	0.017717353967421762	0.0
28	0.0	0.0	0.0	0.02747997758212355	0.0
29	0.0	0.0	0.0	0.04302785963516714	0.0
30	0.0	0.0	0.0	0.05929889899300345	0.0
31	0.0	0.0	0.0	0.08027046083199248	0.0
32	0.0	0.0	0.0	0.09943412940899969	0.0
33	0.0	0.0	0.0	0.1319762081246723	0.0
34	0.0	0.0	0.0	0.1804277475457849	0.0
35	0.0	0.0	0.0	0.24948927015349015	0.0
36	0.0	0.0	0.0	0.3912281018928642	0.0
37	0.0	0.0	0.0	0.5799721584437655	0.0
38	0.0	0.0	0.0	0.8356082656879937	0.0
39	0.0	0.0	0.0	1.1552437944063783	0.0
40	0.0	0.0	0.0	1.522969283893479	0.0
41	0.0	0.0	0.0	1.9033500262144523	0.0
42	0.0	0.0	0.0	2.3752101675917054	0.0
43	0.0	0.0	0.0	2.8951602697376746	0.0
44	0.0	0.0	0.0	3.5279229114313093	0.0
45	0.0	0.0	0.0	4.21673024424638	0.0
46	0.0	0.0	0.0	5.022327481785475	0.0
47	0.0	0.0	0.0	5.873483629526513	0.0
48	0.0	0.0	0.0	6.696436642380633	0.0
49	0.0	0.0	0.0	7.482508632690326	0.0
50	0.0	0.0	0.0	8.159745448628714	0.0
51	0.0	0.0	0.0	8.830835427476362	0.0
52	0.0	0.0	0.0	9.5427837940448	0.0
53	0.0	0.0	0.0	10.267748992099506	0.0
54	0.0	0.0	0.0	11.019109431779148	0.0
55	0.0	0.0	0.0	11.886898197530417	0.0
56	0.0	0.0	0.0	12.703704373293801	0.0
57	0.0	0.0	0.0	13.46121165006418	0.0
58	0.0	0.0	0.0	14.212210511091426	0.0
59	0.0	0.0	0.0	14.95525464176595	0.0
60	0.0	0.0	0.0	15.737349266899283	0.0
61	0.0	0.0	0.0	16.446766582900946	0.0
62	0.0	0.0	0.0	17.122557084229747	0.0
63	0.0	0.0	0.0	17.849330175546434	0.0
64	0.0	0.0	0.0	18.544645924104643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGAGTC	15	0.0022231129	70.00001	61
GATAACT	20	0.006946483	52.500004	3
ACGGAGC	20	0.006946483	52.500004	3
ATAAGTT	20	0.006946483	52.500004	65
GTACTAG	20	0.006946483	52.500004	1
CTCGCTT	35	0.0012541158	40.000004	18
TCGTTCG	45	9.1837835E-5	38.88889	23
TTCGGTA	45	9.1837835E-5	38.88889	26
CGGTAAG	45	9.1837835E-5	38.88889	28
GAAATCG	65	4.8522816E-7	37.69231	48
CGTGCGA	40	0.0024162682	35.000004	10
TACGGAC	40	0.0024162682	35.000004	38
TAACACG	40	0.0024162682	35.000004	4
CGAGAAT	40	0.0024162682	35.000004	14
AATCCGA	65	2.0982823E-5	32.307693	20
ATCCGAG	65	2.0982823E-5	32.307693	21
CGTTCGG	55	2.9876217E-4	31.81818	24
CGTTCAT	55	2.9876217E-4	31.81818	42
CGCAAAA	45	0.0043028602	31.111113	58
TATCGGC	45	0.0043028602	31.111113	12
>>END_MODULE