FastQCFastQC Report
Wed 25 May 2016
SRR1781169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248365
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT34171.3757977170696354No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG3730.15018219153262335No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC2710.1091136029633805No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCACCTCGTATGCCGT2490.10025567209550461No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.00222284670.01
CTCGAAA252.3873766E-455.99999657
TCGTATG5000.052.50000440
AGCGTCA200.006945654352.53
GTCACGA200.006945654352.52
TAGGGCG200.006945654352.55
GTCCCGA200.006945654352.56
GATCTAC200.006945654352.51
CGTCTTC4950.052.32323548
TATGCCG5050.051.98019843
CCGTCTT5000.051.80000347
CGTATGC5100.051.4705941
GCCGTCT4900.051.42857446
CTCGTAT5050.051.2871339
ATCTCGT4350.050.68965537
TCTCGTA4350.050.68965538
TTGGCAT352.0827067E-550.029
TGCCGTC5250.049.33333245
ATGCCGT5350.049.0654244
GCATCTC4500.049.035
Produced by FastQC (version 0.11.5)