FastQCFastQC Report
Wed 25 May 2016
SRR1781168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246284
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC200.00694558452.57
CGGAATC200.00694558452.520
CGTAGCG200.00694558452.515
GGCTAAA200.00694558452.51
AAAGCGA350.001253848840.025
AACCGTA400.002415755835.010
ACCGACC400.002415755835.08
TGTTCCA400.002415755835.07
GGTCTAT400.002415755835.038
GTAAGGT400.002415755835.019
GATCTGA450.004301953631.11110910
ATCGGTT450.004301953631.11110925
AATCGGC450.004301953631.11110923
CGGGAAT604.9730623E-429.16666613
ATTCGGG755.570516E-528.045
GTGTTCC500.00719954527.9999986
ACAGTGC500.00719954527.9999988
ACAAAAG500.00719954527.99999846
GTACAGG500.00719954527.9999981
GTCTATC500.00719954527.99999839