FastQCFastQC Report
Wed 25 May 2016
SRR1781168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222365
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT28571.2848245002585839No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3340.1502034942549412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG150.00222253970.032
TCTATCG150.00222253970.031
CGATGTA352.964989E-760.00000420
CGCAAAG252.3868309E-455.99999612
TCTCGTA4000.055.12499638
GCCGTCT4300.054.53488546
CCGTCTT4350.053.90804347
TATGCCG4500.053.66666843
CGTATGC4600.053.2608741
TCGTATG4600.053.2608740
ATCTCGT4150.053.13252637
CGTGCCC200.006944701552.512
GCGAAAG200.006944701552.536
GCGCGAT407.45682E-752.517
GAGCGAA200.006944701552.534
CTCGCTA200.006944701552.513
CGCAAAT200.006944701552.538
CTCGTAT4550.052.30769339
CGTCTTC4450.051.9101148
GTATCTC4300.051.2790735
Produced by FastQC (version 0.11.5)