FastQCFastQC Report
Wed 25 May 2016
SRR1781167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252568
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTT150.00222288970.000011
GTACGCT150.00222288970.000016
ACGTTTG200.0069457952.560
ACAATCG459.181519E-538.88888538
CATCGTT459.181519E-538.88888532
GATTCCG459.181519E-538.88888544
GAATTCG556.6784796E-638.18181623
TTCGAAC556.6784796E-638.18181626
TCGAACA556.6784796E-638.18181627
TCGTTGT501.7067141E-435.034
ATTCCGC501.7067141E-435.045
GCGGAAT400.002415872935.019
CGGTAAG400.002415872935.028
TACCGCG400.002415872935.060
AGTCATA552.986893E-431.8181810
CGGATAG552.986893E-431.8181830
GCTAGTC552.986893E-431.818187
CGTATTG450.004302160331.11110957
CCGGAAT450.004302160331.11110956
CGAGAAT450.004302160331.11110914