##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781167_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252568
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38015900668335	34.0	33.0	34.0	23.0	34.0
2	31.55426261442463	34.0	33.0	34.0	23.0	34.0
3	31.510436793259636	34.0	33.0	34.0	23.0	34.0
4	31.663397580057648	34.0	34.0	34.0	23.0	34.0
5	31.67586946881632	34.0	34.0	34.0	23.0	34.0
6	34.535843812359445	38.0	35.0	38.0	26.0	38.0
7	34.55644816445472	38.0	34.0	38.0	26.0	38.0
8	34.29549665832568	38.0	34.0	38.0	24.0	38.0
9	34.540876120490324	38.0	35.0	38.0	26.0	38.0
10-11	34.407571426308955	38.0	34.0	38.0	25.0	38.0
12-13	34.38909917329194	38.0	34.0	38.0	25.0	38.0
14-15	34.27005994425264	38.0	34.0	38.0	24.0	38.0
16-17	34.23888022235596	37.5	34.0	38.0	24.0	38.0
18-19	34.28962497228469	37.5	34.0	38.0	24.0	38.0
20-21	34.21330889107092	37.0	34.0	38.0	24.0	38.0
22-23	34.29157494219379	37.5	34.0	38.0	24.5	38.0
24-25	34.261808701023085	37.5	34.0	38.0	24.0	38.0
26-27	34.200522235595955	37.0	34.0	38.0	24.0	38.0
28-29	34.295514475309616	37.0	34.0	38.0	24.5	38.0
30-31	34.339655063190904	38.0	34.0	38.0	24.5	38.0
32-33	34.288837065661525	37.5	34.0	38.0	24.0	38.0
34-35	34.3288896455608	38.0	34.0	38.0	24.5	38.0
36-37	34.1833664597257	37.0	34.0	38.0	24.0	38.0
38-39	34.190782284374905	37.0	34.0	38.0	24.0	38.0
40-41	34.17468760888157	37.0	34.0	38.0	24.0	38.0
42-43	34.24335426499002	37.0	34.0	38.0	24.0	38.0
44-45	34.26810799467866	37.0	34.0	38.0	24.0	38.0
46-47	34.24811139970225	37.0	34.0	38.0	24.0	38.0
48-49	34.216474375217764	37.0	34.0	38.0	24.0	38.0
50-51	34.088287114757215	37.0	34.0	38.0	24.0	38.0
52-53	34.16662047448608	37.0	34.0	38.0	24.0	38.0
54-55	34.132354455037856	37.0	34.0	38.0	24.0	38.0
56-57	34.11398514459472	37.0	34.0	38.0	24.0	38.0
58-59	34.10294851287574	37.0	34.0	38.0	24.0	38.0
60-61	34.07807996262393	37.0	34.0	38.0	24.0	38.0
62-63	34.04775545595642	37.0	34.0	38.0	24.0	38.0
64-65	34.04645283646384	37.0	34.0	38.0	24.0	38.0
66-67	33.950951822875425	37.0	34.0	38.0	23.0	38.0
68-69	33.742077381140916	37.0	34.0	38.0	22.5	38.0
70-71	33.919601849798866	37.0	34.0	38.0	23.0	38.0
72-73	33.887869801400015	37.0	34.0	38.0	23.0	38.0
74-75	33.901525529758324	37.0	34.0	38.0	23.5	38.0
76	32.540769218586675	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	28.0
14	92.0
15	362.0
16	822.0
17	1384.0
18	1859.0
19	2033.0
20	1969.0
21	1962.0
22	2054.0
23	2357.0
24	2684.0
25	3191.0
26	3770.0
27	4580.0
28	5498.0
29	6435.0
30	7987.0
31	9435.0
32	11634.0
33	14455.0
34	18478.0
35	24819.0
36	38003.0
37	86667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.96754933324887	17.915967185074912	9.529314877577523	25.587168604098697
2	23.439628139748503	20.16803395521206	31.414510151721515	24.977827753317918
3	20.247616483481675	21.741471603686925	28.878955370434895	29.1319565423965
4	14.292784517436887	16.390833359728866	36.82057901238478	32.49580311044946
5	14.164106300085521	33.73507332678724	35.62446549048177	16.476354882645467
6	30.70103892813025	36.8574007791961	17.822527002628995	14.619033290044662
7	29.76940863450635	29.045247220550507	21.597352000253398	19.587992144689746
8	25.970035792341072	34.30957207563903	19.40269551170378	20.317696620316113
9	25.23201672420893	15.330920781730068	21.10599917645941	38.3310633176016
10-11	25.725547179373475	24.03709100123531	27.351643913718288	22.885717905672927
12-13	25.89678819169491	22.29181844097431	26.254909568908175	25.5564837984226
14-15	23.971960026606695	23.68629438408666	23.380436159766873	28.961309429539767
16-17	24.315233917202498	26.179484336891452	24.826779322796234	24.678502423109816
18-19	23.560981597035255	26.09534857939248	26.153946659909412	24.189723163662855
20-21	23.76884640967977	26.171961610338602	25.88807766621266	24.171114313768964
22-23	24.24317411548573	25.708522061385448	26.154738525862342	23.893565297266477
24-25	23.861890659149218	25.926285198441608	25.915001108612334	24.296823033796837
26-27	23.1842513699281	25.85323556428368	26.396455607994678	24.566057457793544
28-29	23.497038421336036	25.845910804219063	27.117251591650565	23.539799182794336
30-31	24.167550916980773	25.66536536695068	26.321822241930885	23.845261474137658
32-33	23.519804567482815	26.18839282886193	26.06030851097526	24.23149409267999
34-35	24.19882962212157	25.354360013936837	26.339243292895382	24.107567071046212
36-37	24.28375724557347	25.415135725824335	25.85184979886605	24.44925722973615
38-39	23.667685534192774	26.05080611954008	26.5724478160337	23.70906053023344
40-41	23.67441639479269	24.965751797535713	27.465276677963956	23.894555129707644
42-43	23.4263643850369	25.239143517785312	26.771008203731274	24.563483893446517
44-45	22.950254980836842	25.05384688479934	27.09408951252732	24.901808621836498
46-47	23.113775300117194	24.689588546450857	27.3878717810649	24.808764372367044
48-49	22.45850622406639	24.745415096132525	27.945543378416904	24.850535301384184
50-51	22.478104906401445	24.855484463590003	27.631370561591336	25.03504006841722
52-53	22.072669538500524	24.8723116150898	28.287035570618606	24.767983275791075
54-55	21.582504513635932	25.300117196161032	28.179341801019923	24.93803648918311
56-57	21.502215253774246	25.19133536843689	28.058891304089606	25.247558073699256
58-59	21.70845417561642	24.974709731061992	27.80108287883437	25.51575321448722
60-61	21.311770738061583	25.06899159430963	27.69839290168549	25.920844765943297
62-63	21.07674186108664	24.729626733447493	28.17009314341711	26.023538262048756
64-65	21.833723987203445	24.808764372367044	27.38688194862374	25.97062969180577
66-67	21.907806823918357	24.593227553030378	27.180456709592487	26.31850891345878
68-69	21.858909004523543	24.57244103061558	26.7512645134469	26.817385451413976
70-71	22.33007017925901	24.218476248923555	27.10225979193681	26.349193779880625
72-73	22.615295682746826	23.775577270279687	26.913544075258937	26.69558297171455
74-75	22.601042095594057	24.217438472015456	27.028958537898706	26.152560894491785
76	22.8409774793323	24.263564663773714	26.73220677203763	26.163251084856356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	3.0
14	11.5
15	14.0
16	24.5
17	38.0
18	45.5
19	68.5
20	109.0
21	134.0
22	170.5
23	290.0
24	510.0
25	647.0
26	797.5
27	1089.5
28	1410.5
29	1590.0
30	1892.0
31	2430.5
32	3291.0
33	3915.0
34	4376.5
35	5432.0
36	6850.5
37	7675.0
38	8342.0
39	10042.0
40	11872.5
41	13328.0
42	13986.0
43	14459.5
44	15057.0
45	15356.0
46	15531.0
47	15304.0
48	14355.5
49	13124.5
50	12615.0
51	11654.5
52	9797.0
53	8542.5
54	8185.0
55	7647.0
56	6582.0
57	5606.5
58	5158.0
59	4743.5
60	4128.0
61	3645.5
62	3364.0
63	3226.0
64	2959.0
65	2743.0
66	2615.0
67	2574.0
68	2472.5
69	2297.0
70	2152.5
71	2082.0
72	2016.5
73	1854.5
74	1632.5
75	1507.0
76	1436.5
77	1231.5
78	981.0
79	865.0
80	784.5
81	601.5
82	420.0
83	341.0
84	278.0
85	187.5
86	121.5
87	83.0
88	60.0
89	30.5
90	19.5
91	15.5
92	16.0
93	9.0
94	2.5
95	4.5
96	6.0
97	3.5
98	1.0
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	3.9593297646574386E-4
58-59	1.9796648823287193E-4
60-61	3.9593297646574386E-4
62-63	1.9796648823287193E-4
64-65	0.0
66-67	1.9796648823287193E-4
68-69	1.9796648823287193E-4
70-71	1.9796648823287193E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	252568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.67804073684545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77777501539882	36.375570062305094
2	14.396338748328908	14.591564830984783
3	5.617303441705503	8.540217979499209
4	2.326750288556721	4.716605836317777
5	1.1945130131189825	3.0267789569767904
6	0.7366501115220946	2.2399190616311078
7	0.5489493611555017	1.947377466097266
8	0.4512895728216188	1.8296377084454065
9	0.3692958306294384	1.6843670233727273
>10	2.522578965281307	23.151539885356193
>50	0.055432683402138944	1.724702683686713
>100	0.0031229680789937432	0.17171850532691751
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.9593297646574386E-4	0.0
2	0.0	0.0	0.0	3.9593297646574386E-4	0.0
3	0.0	0.0	0.0	3.9593297646574386E-4	0.0
4	0.0	0.0	0.0	0.0011877989293972316	0.0
5	0.0	0.0	0.0	0.0019796648823287195	0.0
6	0.0	0.0	0.0	0.0019796648823287195	0.0
7	0.0	0.0	0.0	0.002375597858794463	0.0
8	0.0	0.0	0.0	0.002375597858794463	0.0
9	0.0	0.0	0.0	0.003167463811725951	0.0
10	3.9593297646574386E-4	0.0	0.0	0.003167463811725951	0.0
11	3.9593297646574386E-4	0.0	0.0	0.003167463811725951	0.0
12	3.9593297646574386E-4	0.0	0.0	0.003167463811725951	0.0
13	3.9593297646574386E-4	0.0	0.0	0.003563396788191695	0.0
14	3.9593297646574386E-4	0.0	0.0	0.003563396788191695	0.0
15	3.9593297646574386E-4	0.0	0.0	0.003563396788191695	0.0
16	3.9593297646574386E-4	0.0	0.0	0.003563396788191695	0.0
17	3.9593297646574386E-4	0.0	0.0	0.004751195717588926	0.0
18	3.9593297646574386E-4	0.0	0.0	0.004751195717588926	0.0
19	3.9593297646574386E-4	0.0	0.0	0.0055430616705204145	0.0
20	3.9593297646574386E-4	0.0	0.0	0.006334927623451902	0.0
21	3.9593297646574386E-4	0.0	0.0	0.00712679357638339	0.0
22	3.9593297646574386E-4	0.0	0.0	0.008710525482246365	0.0
23	3.9593297646574386E-4	0.0	0.0	0.009502391435177853	0.0011877989293972316
24	3.9593297646574386E-4	0.0	0.0	0.010294257388109341	0.0011877989293972316
25	3.9593297646574386E-4	0.0	0.0	0.010690190364575084	0.0011877989293972316
26	3.9593297646574386E-4	0.0	0.0	0.011086123341040829	0.0011877989293972316
27	3.9593297646574386E-4	0.0	0.0	0.020588514776218682	0.0011877989293972316
28	3.9593297646574386E-4	0.0	0.0	0.034446168952519715	0.0011877989293972316
29	3.9593297646574386E-4	0.0	0.0	0.05107535396408096	0.0011877989293972316
30	3.9593297646574386E-4	0.0	0.0	0.060577745399258816	0.0011877989293972316
31	3.9593297646574386E-4	0.0	0.0	0.08274999208134047	0.0011877989293972316
32	3.9593297646574386E-4	0.0	0.0	0.10769376959868233	0.0011877989293972316
33	3.9593297646574386E-4	0.0	0.0	0.14491146938646227	0.0011877989293972316
34	3.9593297646574386E-4	0.0	0.0	0.20152988502106364	0.0011877989293972316
35	3.9593297646574386E-4	0.0	0.0	0.28586360900826707	0.0011877989293972316
36	3.9593297646574386E-4	0.0	0.0	0.4485920623356878	0.0011877989293972316
37	7.918659529314877E-4	0.0	0.0	0.694070507744449	0.0011877989293972316
38	7.918659529314877E-4	0.0	0.0	0.9811219156821134	0.0011877989293972316
39	7.918659529314877E-4	0.0	0.0	1.3406290583130087	0.0011877989293972316
40	7.918659529314877E-4	0.0	0.0	1.7231003135789174	0.0011877989293972316
41	7.918659529314877E-4	0.0	0.0	2.101216306103703	0.0011877989293972316
42	7.918659529314877E-4	0.0	0.0	2.5608944917804313	0.0011877989293972316
43	7.918659529314877E-4	0.0	0.0	3.11361692692661	0.0011877989293972316
44	7.918659529314877E-4	0.0	0.0	3.736419498907225	0.0011877989293972316
45	7.918659529314877E-4	0.0	0.0	4.484336891451015	0.0011877989293972316
46	7.918659529314877E-4	0.0	0.0	5.2773906433119	0.0011877989293972316
47	7.918659529314877E-4	0.0	0.0	6.145275727724811	0.0011877989293972316
48	7.918659529314877E-4	0.0	0.0	6.968816318773558	0.0011877989293972316
49	7.918659529314877E-4	0.0	0.0	7.744844952646416	0.0011877989293972316
50	7.918659529314877E-4	0.0	0.0	8.437331728485002	0.0011877989293972316
51	7.918659529314877E-4	0.0	0.0	9.097747933229863	0.0011877989293972316
52	7.918659529314877E-4	0.0	0.0	9.78548351335086	0.0011877989293972316
53	7.918659529314877E-4	0.0	0.0	10.40868201830794	0.0011877989293972316
54	7.918659529314877E-4	0.0	0.0	11.065138893288145	0.0011877989293972316
55	7.918659529314877E-4	0.0	0.0	11.818203414525989	0.0011877989293972316
56	7.918659529314877E-4	0.0	0.0	12.600566976022298	0.0011877989293972316
57	7.918659529314877E-4	0.0	0.0	13.395204459789047	0.0011877989293972316
58	7.918659529314877E-4	0.0	0.0	14.141538120426974	0.0011877989293972316
59	7.918659529314877E-4	0.0	0.0	14.822542839948053	0.0011877989293972316
60	7.918659529314877E-4	0.0	0.0	15.534826264609928	0.0011877989293972316
61	7.918659529314877E-4	0.0	0.0	16.21543505115454	0.0011877989293972316
62	7.918659529314877E-4	0.0	0.0	16.820816572170664	0.0011877989293972316
63	7.918659529314877E-4	0.0	0.0	17.435304551645498	0.0011877989293972316
64	7.918659529314877E-4	0.0	0.0	18.109182477590192	0.0011877989293972316
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTT	15	0.002222889	70.00001	1
GTACGCT	15	0.002222889	70.00001	6
ACGTTTG	20	0.00694579	52.5	60
ACAATCG	45	9.181519E-5	38.888885	38
CATCGTT	45	9.181519E-5	38.888885	32
GATTCCG	45	9.181519E-5	38.888885	44
GAATTCG	55	6.6784796E-6	38.181816	23
TTCGAAC	55	6.6784796E-6	38.181816	26
TCGAACA	55	6.6784796E-6	38.181816	27
TCGTTGT	50	1.7067141E-4	35.0	34
ATTCCGC	50	1.7067141E-4	35.0	45
GCGGAAT	40	0.0024158729	35.0	19
CGGTAAG	40	0.0024158729	35.0	28
TACCGCG	40	0.0024158729	35.0	60
AGTCATA	55	2.986893E-4	31.81818	10
CGGATAG	55	2.986893E-4	31.81818	30
GCTAGTC	55	2.986893E-4	31.81818	7
CGTATTG	45	0.0043021603	31.111109	57
CCGGAAT	45	0.0043021603	31.111109	56
CGAGAAT	45	0.0043021603	31.111109	14
>>END_MODULE