FastQCFastQC Report
Wed 25 May 2016
SRR1781167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227473
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT40571.7835083724222216No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3980.17496582011931086No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2340.10286935152743404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCGT150.002222604470.025
CGTATGC5850.056.8376141
TATGCCG5900.056.35593443
TCGTATG5900.056.35593440
CTCGTAT5800.056.1206939
TTTACGA252.386948E-455.99999648
GTTTACG252.386948E-455.99999647
GTCCATA252.386948E-455.99999629
CCGTCTT5900.055.16949547
TCTCGTA5050.054.75247638
ATGCCGT6100.054.50819844
TGCCGTC6100.054.50819845
AAACTCG452.6906491E-854.44444736
AACTCGT452.6906491E-854.44444737
CGTCTTC6100.053.36065748
GCCGTCT6100.053.36065746
GTATGCC6250.053.242
ATCTCGT5200.053.17307737
CTTGAAA5600.053.12500457
AATCTCG5250.052.66666836