##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781167_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227473
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.28742312274424	34.0	34.0	34.0	33.0	34.0
2	33.3122656315255	34.0	34.0	34.0	33.0	34.0
3	33.35714128709781	34.0	34.0	34.0	33.0	34.0
4	33.42874978568885	34.0	34.0	34.0	34.0	34.0
5	33.406215243127754	34.0	34.0	34.0	34.0	34.0
6	36.832661458722576	38.0	37.0	38.0	34.0	38.0
7	36.75211123957568	38.0	37.0	38.0	34.0	38.0
8	36.55287440707249	38.0	37.0	38.0	34.0	38.0
9	36.509357154475474	38.0	37.0	38.0	34.0	38.0
10-11	36.431147872494755	38.0	37.0	38.0	34.0	38.0
12-13	36.36460151314661	38.0	37.0	38.0	34.0	38.0
14-15	36.372516738250255	38.0	37.0	38.0	34.0	38.0
16-17	36.30135005033564	38.0	37.0	38.0	34.0	38.0
18-19	36.30518786845032	38.0	37.0	38.0	34.0	38.0
20-21	36.25039235425743	38.0	37.0	38.0	34.0	38.0
22-23	36.30642317989388	38.0	37.0	38.0	34.0	38.0
24-25	36.295525183208554	38.0	37.0	38.0	34.0	38.0
26-27	36.0600818558686	38.0	37.0	38.0	33.0	38.0
28-29	36.02913532595077	38.0	37.0	38.0	32.0	38.0
30-31	36.069225798226604	38.0	37.0	38.0	32.5	38.0
32-33	36.04132578371939	38.0	37.0	38.0	32.0	38.0
34-35	36.04256988741521	38.0	37.0	38.0	32.0	38.0
36-37	36.01113318943347	38.0	37.0	38.0	32.0	38.0
38-39	35.86055268097752	38.0	36.5	38.0	31.0	38.0
40-41	35.95411982960615	38.0	37.0	38.0	32.0	38.0
42-43	35.98460476627996	38.0	37.0	38.0	32.0	38.0
44-45	35.95052819455496	38.0	37.0	38.0	32.0	38.0
46-47	35.971401001437535	38.0	37.0	38.0	32.0	38.0
48-49	35.95658605636713	38.0	37.0	38.0	32.0	38.0
50-51	35.84942168960712	38.0	36.5	38.0	31.5	38.0
52-53	35.87579405028289	38.0	37.0	38.0	32.0	38.0
54-55	35.88768557147442	38.0	37.0	38.0	32.0	38.0
56-57	35.84442549225622	38.0	36.0	38.0	31.5	38.0
58-59	35.82983255155557	38.0	36.5	38.0	31.0	38.0
60-61	35.82247123834477	38.0	36.0	38.0	31.0	38.0
62-63	35.796793465598114	38.0	36.0	38.0	31.0	38.0
64-65	35.73728530418995	38.0	36.0	38.0	31.0	38.0
66-67	35.69727176412145	38.0	36.0	38.0	31.0	38.0
68-69	35.60263635684235	38.0	36.0	38.0	31.0	38.0
70-71	35.4433954799031	38.0	36.0	38.0	30.0	38.0
72-73	35.304143788493576	38.0	36.0	38.0	28.5	38.0
74-75	35.3072760283638	38.0	36.0	38.0	28.5	38.0
76	34.385953497777756	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	3.0
21	12.0
22	32.0
23	79.0
24	150.0
25	377.0
26	661.0
27	1109.0
28	1828.0
29	3123.0
30	4629.0
31	6976.0
32	9796.0
33	13637.0
34	18555.0
35	26080.0
36	38751.0
37	101672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.390323247154605	18.050933517384482	9.152294997648072	28.406448237812835
2	23.08405832780154	19.998857007205253	31.730798820079748	25.186285844913463
3	20.446382647610925	21.41484923485425	29.8329911681826	28.30577694935223
4	14.25399937575009	17.74760081416256	35.58664105190506	32.41175875818229
5	14.026719654640333	33.38637992201272	36.55774531482857	16.029155108518374
6	30.715733295819724	38.256408452871334	16.802873308040954	14.224984943267993
7	28.86408496832591	28.60823042734742	21.005569891811334	21.522114712515332
8	25.32476381812347	35.948442232704544	18.39383135580926	20.33296259336273
9	24.500929780677268	15.344238656895543	19.969842574723152	40.18498898770403
10-11	25.369164692073344	25.10935363757457	27.159487060002725	22.36199461034936
12-13	25.269812241452833	21.981729699788545	26.376536995599476	26.371921063159142
14-15	23.34496841383373	22.953273575325422	22.84600809766434	30.85574991317651
16-17	24.912407186787004	26.95638603262805	23.89997933820717	24.231227442377776
18-19	24.37607979848158	25.730306453952778	25.214201245862146	24.6794125017035
20-21	23.18121271535523	25.67469545836209	24.89921880838605	26.24487301789663
22-23	23.431572098666656	26.413024842508783	25.17551533588602	24.979887722938546
24-25	24.57676295648275	26.578099378827382	24.94405929494929	23.901078369740585
26-27	23.787658315492386	26.72361115385123	25.52808465180483	23.960645878851555
28-29	22.98734355286122	25.398398930862125	27.360170218003894	24.25408729827276
30-31	23.282323616429203	25.348502899245183	26.586232212174632	24.78294127215098
32-33	22.918104566256215	26.780980599895372	26.70954355022354	23.59137128362487
34-35	23.45355272933491	25.033960074382456	28.014973205611216	23.497513990671422
36-37	23.444760477067607	27.410286055927518	25.264097277479085	23.88085618952579
38-39	22.758525187604683	25.80350195407807	26.842306559459807	24.595666298857445
40-41	22.63697230000923	24.798767326232124	27.556017637258044	25.0082427365006
42-43	24.100222883594977	24.840310718195127	26.89989581181063	24.159570586399266
44-45	22.420023475313553	25.93648476962101	27.333573654895304	24.30991810017013
46-47	23.632475062974507	24.616547897992287	26.502705815635263	25.248271223397943
48-49	23.017896629490092	24.16088942423936	27.099040325665026	25.72217362060552
50-51	21.764561068786186	24.4727945734219	28.00398289027709	25.758661467514827
52-53	21.420784005134674	24.17100051434676	29.6729721769177	24.735243303600868
54-55	20.803787702276754	24.765356767616375	28.699010432007316	25.731845098099555
56-57	21.794894339108378	24.605997195271527	27.35687312340366	26.242235342216443
58-59	20.840495355492738	24.579400632162937	29.066966189393906	25.513137822950416
60-61	21.700377627234882	25.47445191297429	27.094864005838055	25.730306453952778
62-63	19.98676766033771	26.76383570797413	26.91945857310538	26.329938058582776
64-65	20.236687431035772	27.038373785020642	26.222672580921692	26.502266203021897
66-67	20.46836327827918	27.05727712739534	25.6909611250566	26.78339846926888
68-69	20.55782444509898	27.249607645742575	25.27640643065331	26.916161478505142
70-71	20.929297103392493	26.49655123904815	25.358833795659265	27.215317861900097
72-73	20.63981219749157	25.144302840337097	25.878455904656818	28.33742905751452
74-75	20.416269183595414	25.414444791249952	26.164204103344137	28.005081921810497
76	20.848628188839992	25.157711025044733	26.142882891595924	27.850777894519354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	6.5
15	15.0
16	20.5
17	23.0
18	30.5
19	59.0
20	115.0
21	150.0
22	185.5
23	295.0
24	451.5
25	534.0
26	679.0
27	922.5
28	1216.5
29	1412.0
30	1621.0
31	2053.5
32	2856.0
33	3435.0
34	3719.5
35	4628.5
36	6064.0
37	6875.0
38	7973.5
39	10194.0
40	12020.0
41	12851.5
42	12979.0
43	13066.0
44	13282.0
45	13498.0
46	13585.0
47	13422.0
48	12549.5
49	11359.0
50	10878.0
51	10338.0
52	8788.0
53	7576.0
54	7374.0
55	6824.0
56	5839.5
57	5024.0
58	4643.0
59	4204.5
60	3495.5
61	3077.0
62	2929.0
63	2781.5
64	2555.5
65	2437.0
66	2361.5
67	2326.0
68	2229.5
69	2074.0
70	1967.0
71	1919.0
72	1871.0
73	1693.5
74	1499.5
75	1435.0
76	1329.0
77	1104.5
78	927.0
79	868.0
80	737.0
81	542.0
82	408.5
83	339.0
84	272.0
85	162.0
86	96.5
87	74.0
88	54.5
89	25.0
90	12.0
91	5.0
92	1.0
93	3.5
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	227473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.29217093896858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.92042892828855	26.80669793777723
2	19.690692337374845	17.049056371525413
3	7.697150632628608	9.996790827922435
4	3.073782976908548	5.322829522624663
5	1.5749710595259856	3.409195816646371
6	1.0398261540648672	2.7009798965151908
7	0.761591421434229	2.307966220166789
8	0.579824935518593	2.0081504178517893
9	0.4955421515465383	1.9307785978995309
>10	3.0727675216799692	23.80853991462723
>50	0.08428278397205467	2.255652319176342
>100	0.008123641828631775	0.6198537848447948
>500	0.0	0.0
>1k	0.001015455228578972	1.7835083724222216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	4057	1.7835083724222216	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG	398	0.17496582011931086	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC	234	0.10286935152743404	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.3961261336510266E-4	0.0
2	0.0	0.0	0.0	4.3961261336510266E-4	0.0
3	0.0	0.0	0.0	4.3961261336510266E-4	0.0
4	0.0	0.0	0.0	0.0017584504534604107	0.0
5	0.0	0.0	0.0	0.002637675680190616	0.0
6	0.0	0.0	0.0	0.002637675680190616	0.0
7	0.0	0.0	0.0	0.002637675680190616	0.0
8	0.0	0.0	0.0	0.002637675680190616	0.0
9	0.0	0.0	0.0	0.002637675680190616	0.0
10	0.0	0.0	0.0	0.002637675680190616	0.0
11	0.0	0.0	0.0	0.002637675680190616	0.0
12	0.0	0.0	0.0	0.003077288293555719	0.0
13	0.0	0.0	0.0	0.0035169009069208213	0.0
14	0.0	0.0	0.0	0.0035169009069208213	0.0
15	0.0	0.0	0.0	0.0035169009069208213	0.0
16	0.0	0.0	0.0	0.0035169009069208213	0.0
17	0.0	0.0	0.0	0.0048357387470161295	0.0
18	0.0	0.0	0.0	0.0048357387470161295	0.0
19	0.0	0.0	0.0	0.005714963973746334	0.0
20	0.0	0.0	0.0	0.006154576587111438	0.0
21	0.0	0.0	0.0	0.00659418920047654	0.0
22	0.0	0.0	0.0	0.007473414427206745	0.0
23	0.0	0.0	0.0	0.007913027040571848	0.0
24	0.0	0.0	0.0	0.007913027040571848	0.0
25	0.0	0.0	0.0	0.007913027040571848	0.0
26	0.0	0.0	0.0	0.00835263965393695	0.0
27	0.0	0.0	0.0	0.01978256760142962	0.0
28	0.0	0.0	0.0	0.0325313333890176	0.0
29	0.0	0.0	0.0	0.05143467576371701	0.0
30	0.0	0.0	0.0	0.06242499109784458	0.0
31	0.0	0.0	0.0	0.08440562176609971	0.0
32	0.0	0.0	0.0	0.11034276595464076	0.0
33	0.0	0.0	0.0	0.14990790115750002	0.0
34	0.0	0.0	0.0	0.21057444180188417	0.0
35	0.0	0.0	0.0	0.30553076628874637	0.0
36	0.0	0.0	0.0	0.4734627845942156	0.0
37	0.0	0.0	0.0	0.7341530643197215	0.0
38	0.0	0.0	0.0	1.0352877044748168	0.0
39	0.0	0.0	0.0	1.408958425835154	0.0
40	0.0	0.0	0.0	1.8085662913840324	0.0
41	0.0	0.0	0.0	2.20377803079926	0.0
42	0.0	0.0	0.0	2.680757716300396	0.0
43	0.0	0.0	0.0	3.2597275281022364	0.0
44	0.0	0.0	0.0	3.914310709402874	0.0
45	0.0	0.0	0.0	4.696381548579392	0.0
46	0.0	0.0	0.0	5.52417209954588	0.0
47	0.0	0.0	0.0	6.430653308304722	0.0
48	0.0	0.0	0.0	7.276907589032544	0.0
49	0.0	0.0	0.0	8.083596734557508	0.0
50	0.0	0.0	0.0	8.79708800604907	0.0
51	0.0	0.0	0.0	9.482444070285265	0.0
52	0.0	0.0	0.0	10.182746963375873	0.0
53	0.0	0.0	0.0	10.840407432970066	0.0
54	0.0	0.0	0.0	11.53499536208693	0.0
55	0.0	0.0	0.0	12.31398891296989	0.0
56	0.0	0.0	0.0	13.125953409855235	0.0
57	0.0	0.0	0.0	13.969130402289503	0.0
58	0.0	0.0	0.0	14.738452475678432	0.0
59	0.0	0.0	0.0	15.468209413864503	0.0
60	0.0	0.0	0.0	16.190053325010002	0.0
61	0.0	0.0	0.0	16.904423821728294	0.0
62	0.0	0.0	0.0	17.53306985884039	0.0
63	0.0	0.0	0.0	18.152044418458456	0.0
64	0.0	0.0	0.0	18.849270023255507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTCGT	15	0.0022226044	70.0	25
CGTATGC	585	0.0	56.83761	41
TATGCCG	590	0.0	56.355934	43
TCGTATG	590	0.0	56.355934	40
CTCGTAT	580	0.0	56.12069	39
TTTACGA	25	2.386948E-4	55.999996	48
GTTTACG	25	2.386948E-4	55.999996	47
GTCCATA	25	2.386948E-4	55.999996	29
CCGTCTT	590	0.0	55.169495	47
TCTCGTA	505	0.0	54.752476	38
ATGCCGT	610	0.0	54.508198	44
TGCCGTC	610	0.0	54.508198	45
AAACTCG	45	2.6906491E-8	54.444447	36
AACTCGT	45	2.6906491E-8	54.444447	37
CGTCTTC	610	0.0	53.360657	48
GCCGTCT	610	0.0	53.360657	46
GTATGCC	625	0.0	53.2	42
ATCTCGT	520	0.0	53.173077	37
CTTGAAA	560	0.0	53.125004	57
AATCTCG	525	0.0	52.666668	36
>>END_MODULE