FastQCFastQC Report
Wed 25 May 2016
SRR1781166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129015
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCA200.00693812652.49999621
TACTCGC200.00693812652.49999620
ATAGTGC200.00693812652.4999966
GCATCAG305.8599067E-446.66666825
TTACTCG404.5720495E-543.74999619
AGTTAAT350.001251629640.00000470
TAGAGGT501.7020958E-435.04
GGCGCCA400.002411507534.99999639
TGGTACG806.1780156E-834.99999670
TACTGAC552.97885E-431.8181834
TCGGGCC450.004294433631.11110948
TCTACTG604.9600925E-429.16666832
TATAGTG604.9600925E-429.1666685
GACTATC604.9600925E-429.16666838
CCGGACG500.007187035427.99999828
GAGAGGT500.007187035427.9999986
TTTCAAC500.007187035427.99999842
TATAGAG500.007187035427.9999982
ATAGTAC500.007187035427.9999983
GGACAGA500.007187035427.9999986
Produced by FastQC (version 0.11.5)