##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781166_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129015
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189505096306632	34.0	32.0	34.0	21.0	34.0
2	31.27182885710964	34.0	33.0	34.0	23.0	34.0
3	31.24188660233306	34.0	33.0	34.0	23.0	34.0
4	31.402108281982716	34.0	33.0	34.0	23.0	34.0
5	31.359586094640157	34.0	33.0	34.0	23.0	34.0
6	34.193233344959886	37.0	34.0	38.0	24.0	38.0
7	34.01752509398132	37.0	34.0	38.0	24.0	38.0
8	33.87169708948572	37.0	34.0	38.0	23.0	38.0
9	34.17377049180328	37.0	34.0	38.0	24.0	38.0
10-11	34.04031314188273	37.0	34.0	38.0	23.0	38.0
12-13	34.02689997287137	37.0	34.0	38.0	23.0	38.0
14-15	33.89670580940201	37.0	34.0	38.0	23.0	38.0
16-17	33.86266713172887	37.0	34.0	38.0	22.5	38.0
18-19	33.91888927644072	37.0	34.0	38.0	23.0	38.0
20-21	33.869538425764446	37.0	34.0	38.0	23.0	38.0
22-23	33.9156919738015	37.0	34.0	38.0	23.0	38.0
24-25	33.92337325117235	37.0	34.0	38.0	23.0	38.0
26-27	33.827671201023136	37.0	34.0	38.0	23.0	38.0
28-29	33.9267953338759	37.0	34.0	38.0	23.0	38.0
30-31	33.99456652327248	37.0	34.0	38.0	23.0	38.0
32-33	33.94197573925513	37.0	34.0	38.0	23.0	38.0
34-35	33.94676200441809	37.0	34.0	38.0	23.0	38.0
36-37	33.810963841413795	37.0	34.0	38.0	22.5	38.0
38-39	33.8310932837267	37.0	34.0	38.0	23.0	38.0
40-41	33.82198969112119	37.0	34.0	38.0	23.0	38.0
42-43	33.917548347091426	37.0	34.0	38.0	23.0	38.0
44-45	33.91015773359687	37.0	34.0	38.0	23.0	38.0
46-47	33.919676006665895	37.0	34.0	38.0	23.0	38.0
48-49	33.878684649071815	37.0	34.0	38.0	23.0	38.0
50-51	33.75085067627795	37.0	34.0	38.0	22.5	38.0
52-53	33.83227919234197	37.0	34.0	38.0	23.0	38.0
54-55	33.805468356392666	37.0	34.0	38.0	23.0	38.0
56-57	33.75473782118358	37.0	34.0	38.0	23.0	38.0
58-59	33.76826725574546	37.0	34.0	38.0	23.0	38.0
60-61	33.749920551873814	37.0	34.0	38.0	23.0	38.0
62-63	33.73687555710576	37.0	34.0	38.0	22.5	38.0
64-65	33.7100453435647	37.0	34.0	38.0	22.5	38.0
66-67	33.64043715846995	37.0	34.0	38.0	22.0	38.0
68-69	33.40085648955548	37.0	34.0	38.0	21.5	38.0
70-71	33.5368096732938	37.0	34.0	38.0	22.0	38.0
72-73	33.52729527574313	37.0	34.0	38.0	22.0	38.0
74-75	33.52662481106848	37.0	34.0	38.0	22.0	38.0
76	32.10653799945743	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	32.0
14	77.0
15	269.0
16	624.0
17	1069.0
18	1402.0
19	1429.0
20	1245.0
21	1214.0
22	1130.0
23	1211.0
24	1428.0
25	1606.0
26	2039.0
27	2454.0
28	2810.0
29	3411.0
30	4143.0
31	4941.0
32	6071.0
33	7479.0
34	9528.0
35	12627.0
36	19122.0
37	41647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38166104716506	19.238848195946208	10.374762624501027	26.004728132387704
2	22.13463550749913	20.066658915629965	32.4923458512576	25.306359725613305
3	19.73491454482037	21.966438011083984	28.61837770801845	29.680269736077204
4	14.359570592566756	15.916753865829556	36.47405340464287	33.24962213696082
5	14.241754834709141	33.6782544665349	35.45479207844049	16.62519862031547
6	31.025074603728246	36.300430182536914	17.567724683176376	15.10677053055846
7	30.08099833352711	27.794442506685268	22.09665542766345	20.027903732124173
8	26.165174592101692	33.84102623725923	19.277603379452003	20.716195791187072
9	24.286323295740804	16.015967135604388	21.695151726543425	38.002557842111386
10-11	25.98651319613998	24.074332441964113	26.986396930589468	22.952757431306438
12-13	25.435802038522652	22.60434833158935	26.4535131573848	25.5063364725032
14-15	23.577490989419836	23.917373948765647	23.73289927527807	28.77223578653645
16-17	23.848389722125336	26.48335464868426	25.31798628066504	24.350269348525366
18-19	23.45424950587141	26.022943068635428	26.40894469635314	24.113862729140024
20-21	23.177149943804984	26.33182188117661	26.245785373793744	24.245242801224663
22-23	23.72166027206139	25.75010657675464	26.841064992442735	23.68716815874123
24-25	23.134131690113552	26.15432314072007	26.223694919195445	24.487850249970933
26-27	22.517149168701316	26.198891601751733	26.881370383288765	24.402588846258187
28-29	23.35658644343681	25.990388714490564	27.393713909235363	23.25931093283727
30-31	23.715459442700464	26.06441111498663	26.352362128434674	23.867767313878232
32-33	22.78300972755106	26.42328411425028	26.54885090880905	24.244855249389605
34-35	23.727861101422317	25.633065922567145	26.97128240902221	23.667790566988334
36-37	23.639886834864164	25.536177963802658	26.382203619734142	24.44173158159904
38-39	22.900050381738556	25.713289152424135	27.475487346432587	23.911173119404722
40-41	23.34340968104484	24.943998759834127	27.840561175057164	23.87203038406387
42-43	22.730690229818237	25.06258962136186	27.811494787427822	24.395225361392086
44-45	22.279967445645855	24.72696973220168	28.278494748672635	24.71456807347983
46-47	22.406309343874746	24.45064527380537	28.526527923109718	24.61651745921017
48-49	21.378909429136147	24.478549005929544	29.22334612254389	24.91919544239042
50-51	21.340541797465413	24.707592140448785	28.835406735650892	25.11645932643491
52-53	20.978955935356353	24.675425338138975	29.295043212029608	25.05057551447506
54-55	20.188737743673215	25.16916637600279	29.164825795450138	25.477270084873854
56-57	20.326318645118786	25.26644188660233	29.071038251366122	25.336201216912762
58-59	20.563967616043158	25.03749578535746	28.769246867600152	25.629289730999226
60-61	20.301206453538168	24.727453115735052	28.834743381557885	26.13659704916889
62-63	19.929853117854513	24.546370577064682	29.216757741347905	26.307018563732896
64-65	21.15684222764795	24.34019300081386	28.237414254156494	26.265550517381698
66-67	21.13173325478919	24.376717345724703	27.609687283212352	26.88186211627375
68-69	21.027012362903537	24.49443863116692	27.041816843002753	27.43673216292679
70-71	21.740495291245203	24.11851335116072	27.471611828082004	26.66937952951207
72-73	21.99550439871333	23.525171491687015	27.238693175212187	27.24063093438747
74-75	22.205557493314732	23.86815486571329	27.43014378173081	26.496143859241172
76	22.88261054916095	23.60500716970895	26.93872805487734	26.573654226252764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.5
12	1.0
13	4.0
14	5.0
15	9.5
16	19.5
17	23.0
18	48.0
19	81.5
20	96.5
21	103.0
22	141.5
23	221.0
24	301.0
25	340.0
26	466.5
27	675.0
28	850.0
29	943.0
30	1126.0
31	1410.5
32	1873.0
33	2234.0
34	2387.0
35	2886.0
36	3763.0
37	4294.0
38	4664.0
39	5443.0
40	6480.0
41	7272.5
42	7437.0
43	7542.0
44	7735.5
45	7916.0
46	8008.0
47	7898.5
48	7369.5
49	6555.0
50	6160.0
51	5725.5
52	4863.5
53	4126.0
54	3816.0
55	3509.5
56	2915.0
57	2357.0
58	2087.0
59	1929.0
60	1697.0
61	1548.0
62	1473.0
63	1367.5
64	1240.0
65	1227.0
66	1177.5
67	1119.0
68	1132.5
69	1149.5
70	1127.5
71	1102.0
72	1077.5
73	1003.5
74	933.5
75	913.0
76	846.5
77	734.5
78	591.0
79	493.0
80	451.0
81	338.5
82	234.0
83	200.0
84	178.5
85	126.0
86	73.5
87	52.0
88	42.0
89	24.0
90	12.0
91	7.0
92	6.0
93	4.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.87551835057939E-4
60-61	3.87551835057939E-4
62-63	0.0
64-65	0.0
66-67	3.87551835057939E-4
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	129015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.176956167887454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73729299066044	42.89811262256327
2	13.86146528638235	16.128357167771192
3	5.212038850473641	9.096616672479945
4	2.286262440545186	5.320311591675386
5	1.1537897864289806	3.356198891601752
6	0.8060540655768283	2.813626322520637
7	0.5875534593708782	2.3927450296477155
8	0.41301943856002765	1.9222571018873775
9	0.3703851739344765	1.9393093826299268
>10	1.5641445834499113	13.854202999651202
>50	0.007993924617290859	0.2782622175716002
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.75103670115878E-4	0.0
3	0.0	0.0	0.0	7.75103670115878E-4	0.0
4	0.0	0.0	0.0	0.002325311010347634	0.0
5	0.0	0.0	0.0	0.002325311010347634	0.0
6	0.0	0.0	0.0	0.002325311010347634	0.0
7	0.0	0.0	0.0	0.002325311010347634	0.0
8	0.0	0.0	0.0	0.002325311010347634	0.0
9	0.0	0.0	0.0	0.002325311010347634	0.0
10	0.0	0.0	0.0	0.002325311010347634	0.0
11	0.0	0.0	0.0	0.002325311010347634	0.0
12	0.0	0.0	0.0	0.002325311010347634	0.0
13	0.0	0.0	0.0	0.002325311010347634	0.0
14	0.0	0.0	0.0	0.002325311010347634	0.0
15	0.0	0.0	0.0	0.002325311010347634	0.0
16	0.0	0.0	0.0	0.002325311010347634	0.0
17	0.0	0.0	0.0	0.002325311010347634	0.0
18	0.0	0.0	0.0	0.002325311010347634	0.0
19	0.0	0.0	0.0	0.002325311010347634	0.0
20	0.0	0.0	0.0	0.003100414680463512	0.0
21	0.0	0.0	0.0	0.004650622020695268	0.0
22	0.0	0.0	0.0	0.006200829360927024	0.0
23	0.0	0.0	0.0	0.006975933031042902	0.0
24	0.0	0.0	0.0	0.010851451381622293	0.0
25	0.0	0.0	0.0	0.01162655505173817	0.0
26	0.0	0.0	0.0	0.012401658721854048	0.0
27	0.0	0.0	0.0	0.03100414680463512	0.0
28	0.0	0.0	0.0	0.0457311165368368	0.0
29	0.0	0.0	0.0	0.07130953765066078	0.0
30	0.0	0.0	0.0	0.09688795876448475	0.0
31	0.0	0.0	0.0	0.11471534317714994	0.0
32	0.0	0.0	0.0	0.13874355695074217	0.0
33	0.0	0.0	0.0	0.18137425880711544	0.0
34	0.0	0.0	0.0	0.26973607720032555	0.0
35	0.0	0.0	0.0	0.3976281827694454	0.0
36	0.0	0.0	0.0	0.5813277525869085	0.0
37	0.0	0.0	0.0	0.8843932876022168	0.0
38	0.0	0.0	0.0	1.2944231290935162	0.0
39	0.0	0.0	0.0	1.7687865752044336	0.0
40	0.0	0.0	0.0	2.318335077316591	0.0
41	0.0	0.0	0.0	2.936092702398946	0.0
42	0.0	0.0	0.0	3.6747665000193774	0.0
43	0.0	0.0	0.0	4.5064527380537145	0.0
44	0.0	0.0	0.0	5.366817811882339	0.0
45	0.0	0.0	0.0	6.379878308723792	0.0
46	0.0	0.0	0.0	7.4301437817308065	0.0
47	0.0	0.0	0.0	8.616052397008099	0.0
48	0.0	0.0	0.0	9.743053133356586	0.0
49	0.0	0.0	0.0	10.726659690733635	0.0
50	0.0	0.0	0.0	11.646707747161182	0.0
51	0.0	0.0	0.0	12.596984846723249	0.0
52	0.0	0.0	0.0	13.476727512304771	0.0
53	0.0	0.0	0.0	14.347944037515017	0.0
54	0.0	0.0	0.0	15.281168856334535	0.0
55	0.0	0.0	0.0	16.326008603650738	0.0
56	0.0	0.0	0.0	17.27163508119211	0.0
57	0.0	0.0	0.0	18.27926985234275	0.0
58	0.0	0.0	0.0	19.171414176646127	0.0
59	0.0	0.0	0.0	20.046506220206954	0.0
60	0.0	0.0	0.0	20.84641320776654	0.0
61	0.0	0.0	0.0	21.723055458667595	0.0
62	0.0	0.0	0.0	22.507460372824866	0.0
63	0.0	0.0	0.0	23.239158237414255	0.0
64	0.0	0.0	0.0	24.01116149284967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGCA	20	0.006938126	52.499996	21
TACTCGC	20	0.006938126	52.499996	20
ATAGTGC	20	0.006938126	52.499996	6
GCATCAG	30	5.8599067E-4	46.666668	25
TTACTCG	40	4.5720495E-5	43.749996	19
AGTTAAT	35	0.0012516296	40.000004	70
TAGAGGT	50	1.7020958E-4	35.0	4
GGCGCCA	40	0.0024115075	34.999996	39
TGGTACG	80	6.1780156E-8	34.999996	70
TACTGAC	55	2.97885E-4	31.81818	34
TCGGGCC	45	0.0042944336	31.111109	48
TCTACTG	60	4.9600925E-4	29.166668	32
TATAGTG	60	4.9600925E-4	29.166668	5
GACTATC	60	4.9600925E-4	29.166668	38
CCGGACG	50	0.0071870354	27.999998	28
GAGAGGT	50	0.0071870354	27.999998	6
TTTCAAC	50	0.0071870354	27.999998	42
TATAGAG	50	0.0071870354	27.999998	2
ATAGTAC	50	0.0071870354	27.999998	3
GGACAGA	50	0.0071870354	27.999998	6
>>END_MODULE