FastQCFastQC Report
Wed 25 May 2016
SRR1781166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116604
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT36853.1602689444615963No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3340.28643957325649205No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC2360.2023944290075812No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT2140.18352715172721348No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTAACTCGTATGCCGT1490.12778292339885425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC150.002219880270.08
CCATTCG150.002219880270.067
CTTGAAA4900.055.057
TCGTATG5250.054.66666840
CGTATGC5300.054.15094441
CTCGTAT5300.054.15094439
GCCTTAT4850.054.1237132
TATGCCG5250.054.043
ATCTCGT4900.053.5714337
CCGTCTT5000.053.19999747
TGCCTTA5400.053.14814831
CGTCTTC4950.053.03030448
TCTCGTA4950.053.03030438
GCCGTCT5150.053.00971246
TGCCGTC5250.052.66666845
GACAGTG200.006936458452.50000468
ATCAACG200.006936458452.5000047
TACGAGT200.006936458452.50000444
GCGTGCG200.006936458452.50000423
CGTCCGT200.006936458452.5000046