##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781166_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116604
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25166375081472	34.0	34.0	34.0	32.0	34.0
2	33.27863538129052	34.0	34.0	34.0	33.0	34.0
3	33.34940482316215	34.0	34.0	34.0	33.0	34.0
4	33.40713011560495	34.0	34.0	34.0	34.0	34.0
5	33.398451168055985	34.0	34.0	34.0	34.0	34.0
6	36.81400295015608	38.0	37.0	38.0	34.0	38.0
7	36.70330348873109	38.0	37.0	38.0	34.0	38.0
8	36.506620699118386	38.0	37.0	38.0	34.0	38.0
9	36.45147679324894	38.0	37.0	38.0	34.0	38.0
10-11	36.373293369009644	38.0	37.0	38.0	34.0	38.0
12-13	36.315812493567975	38.0	37.0	38.0	34.0	38.0
14-15	36.32406263936057	38.0	37.0	38.0	34.0	38.0
16-17	36.2297777091695	38.0	37.0	38.0	34.0	38.0
18-19	36.2216690679565	38.0	37.0	38.0	34.0	38.0
20-21	36.17451802682584	38.0	37.0	38.0	33.0	38.0
22-23	36.2461236321224	38.0	37.0	38.0	34.0	38.0
24-25	36.228529896058454	38.0	37.0	38.0	34.0	38.0
26-27	35.99316061198587	38.0	36.5	38.0	32.0	38.0
28-29	35.984117182943976	38.0	37.0	38.0	32.0	38.0
30-31	36.04050032588934	38.0	37.0	38.0	32.0	38.0
32-33	35.97920311481596	38.0	37.0	38.0	32.0	38.0
34-35	35.94759184933621	38.0	37.0	38.0	32.0	38.0
36-37	35.88261980721073	38.0	36.5	38.0	31.5	38.0
38-39	35.64613992658914	38.0	36.0	38.0	31.0	38.0
40-41	35.83855613872595	38.0	36.0	38.0	31.5	38.0
42-43	35.88956210764639	38.0	36.5	38.0	31.5	38.0
44-45	35.83440962574183	38.0	36.0	38.0	31.0	38.0
46-47	35.8589027820658	38.0	36.0	38.0	31.5	38.0
48-49	35.83077767486536	38.0	36.0	38.0	31.0	38.0
50-51	35.705511817776404	38.0	36.0	38.0	31.0	38.0
52-53	35.755167061164286	38.0	36.0	38.0	31.0	38.0
54-55	35.77795787451545	38.0	36.0	38.0	31.0	38.0
56-57	35.72741929950945	38.0	36.0	38.0	31.0	38.0
58-59	35.68912730266543	38.0	36.0	38.0	31.0	38.0
60-61	35.67778120819183	38.0	36.0	38.0	31.0	38.0
62-63	35.66198843950465	38.0	36.0	38.0	31.0	38.0
64-65	35.59018129738259	38.0	36.0	38.0	31.0	38.0
66-67	35.52962162533018	38.0	36.0	38.0	31.0	38.0
68-69	35.38958354773421	38.0	36.0	38.0	31.0	38.0
70-71	35.086262049329356	38.0	36.0	38.0	28.0	38.0
72-73	34.87553600219547	38.0	36.0	38.0	27.0	38.0
74-75	34.874125244417	38.0	36.0	38.0	27.0	38.0
76	33.93652018798669	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	2.0
22	13.0
23	60.0
24	101.0
25	166.0
26	373.0
27	703.0
28	1126.0
29	1890.0
30	2637.0
31	3868.0
32	5436.0
33	7327.0
34	9870.0
35	13624.0
36	19615.0
37	49788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85280093307262	18.897293403313782	10.266371651058282	29.983534012555314
2	21.334602586532196	19.21803711708003	34.774964838255976	24.672395458131795
3	19.26263249974272	21.51555692772118	30.552982745017328	28.668827827518783
4	13.792837295461563	18.97705052999897	34.974786456725326	32.255325717814145
5	13.660766354498987	33.927652567664914	37.23628691983122	15.17529415800487
6	30.314568968474493	38.89060409591438	16.4762786868375	14.318548248773627
7	28.637954101059997	27.887551027409007	20.76515385407019	22.709341017460808
8	25.03430414050976	36.675414222496656	18.414462625638915	19.87581901135467
9	23.890261054509278	15.389695036190867	19.669994168296114	41.05004974100374
10-11	24.921958080340296	25.835734623169017	27.07711570786594	22.165191588624747
12-13	24.417258413090458	21.97008678947549	27.289801379026446	26.3228534184076
14-15	22.210644574800178	23.069963294569654	22.518095434118898	32.201296696511264
16-17	24.921958080340296	27.677866968543103	23.639840828788035	23.760334122328565
18-19	24.719992453089088	25.511989297108162	24.877791499433982	24.890226750368768
20-21	22.44477033377929	25.608898494048233	24.395818325271858	27.55051284690062
22-23	22.734640321086754	27.203612225995677	24.878220301190353	25.183527151727215
24-25	24.67325306164454	27.291087784295563	24.66510582827347	23.37055332578642
26-27	23.940859661761174	27.61011629103633	25.231981750197246	23.21704229700525
28-29	22.474357654968955	25.246989811670268	27.92056876264965	24.358083770711126
30-31	24.72513807416555	24.956691022606428	27.601111454152516	22.717059449075503
32-33	23.874395389523514	25.532142979657646	25.52013653047923	25.07332510033961
34-35	22.85127439881994	24.804895200850744	27.271362903502457	25.072467496826867
36-37	22.3144145998422	26.828410689170184	27.433021165654694	23.42415354533292
38-39	21.765119549929675	25.87089636719152	27.50720386950705	24.856780213371753
40-41	21.990669273781347	24.480292271277143	28.40254193681177	25.126496518129738
42-43	24.055778532468867	24.220009605159344	27.996895475283868	23.72731638708792
44-45	21.494116839902578	26.32757023772769	28.26618297828548	23.912129944084253
46-47	23.604679084765532	23.60682309354739	26.997358581180748	25.79113924050633
48-49	22.70762581043532	23.640269630544406	27.311241466845047	26.340863092175226
50-51	20.607783609481665	23.764193338135915	29.47025831017804	26.15776474220438
52-53	19.97315701005111	23.738036430997223	32.0507872800247	24.238019278926966
54-55	19.1725841309046	23.876110596549	30.254107920826044	26.69719735172035
56-57	21.091043188912902	24.27360982470584	27.90513189942026	26.730215086960996
58-59	19.35568248087544	24.17455661898391	30.914462625638915	25.55529827450173
60-61	21.0022812253439	25.91849336214881	27.36912970395527	25.71009570855202
62-63	18.52938149634661	28.26661178004185	26.986209735515075	26.217796988096463
64-65	18.825254708243286	28.721141641796166	25.83787863195088	26.615725018009673
66-67	18.924736715721586	28.51317278995575	25.02615690713869	27.535933587183976
68-69	19.257486878666256	28.667541422249666	24.62351205790539	27.451459641178687
70-71	19.77505059860725	27.626839559534837	24.78688552708312	27.81122431477479
72-73	20.000171520702548	25.514990909402762	25.3567630613015	29.128074508593187
74-75	20.31362560461048	25.24784741518301	25.600322458920793	28.83820452128572
76	21.45809749236733	24.831052107989436	25.09004836883812	28.62080203080512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	3.5
14	4.5
15	5.5
16	18.0
17	28.0
18	32.0
19	57.5
20	80.0
21	81.0
22	116.0
23	197.5
24	302.5
25	361.0
26	442.0
27	599.5
28	789.0
29	902.0
30	1031.0
31	1224.0
32	1567.5
33	1847.0
34	2023.5
35	2511.0
36	3236.0
37	3650.0
38	3924.5
39	4562.0
40	5574.0
41	6787.5
42	7352.0
43	7660.0
44	7951.0
45	7732.5
46	7531.0
47	7219.5
48	6513.0
49	5743.5
50	5369.0
51	5022.0
52	4269.5
53	3566.5
54	3269.0
55	3051.0
56	2575.0
57	2052.5
58	1788.0
59	1681.5
60	1438.5
61	1226.0
62	1150.0
63	1123.0
64	1154.5
65	1150.5
66	1121.5
67	1155.0
68	1086.5
69	1005.5
70	978.0
71	963.0
72	972.0
73	971.0
74	884.5
75	808.0
76	774.0
77	684.5
78	561.5
79	494.0
80	425.0
81	306.5
82	226.5
83	196.0
84	170.0
85	109.5
86	48.5
87	22.0
88	22.5
89	18.0
90	11.0
91	5.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	116604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.074645809749235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.23466088810443	31.522932317930774
2	19.221292137776768	18.86556207334225
3	6.981458504447513	10.278378100236699
4	3.0931618405186727	6.0718328702274365
5	1.576289254320815	3.8677918424753868
6	1.069500026213236	3.1491200987959247
7	0.7619313912238086	2.617405920894652
8	0.5871764849798158	2.30523824225584
9	0.4316446184226623	1.9064526088298857
>10	2.0219142652429967	15.066378511886386
>50	0.010485294374639569	0.29844602243490786
>100	0.00873774531219964	0.8901924462282598
>500	0.0	0.0
>1k	0.001747549062439928	3.1602689444615963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT	3685	3.1602689444615963	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG	334	0.28643957325649205	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC	236	0.2023944290075812	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT	214	0.18352715172721348	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTAACTCGTATGCCGT	149	0.12778292339885425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.576035127439882E-4	0.0
3	0.0	0.0	0.0	8.576035127439882E-4	0.0
4	0.0	0.0	0.0	0.0017152070254879764	0.0
5	0.0	0.0	0.0	0.0017152070254879764	0.0
6	0.0	0.0	0.0	0.0017152070254879764	0.0
7	0.0	0.0	0.0	0.0017152070254879764	0.0
8	0.0	0.0	0.0	0.0017152070254879764	0.0
9	0.0	0.0	0.0	0.0017152070254879764	0.0
10	0.0	0.0	0.0	0.0017152070254879764	0.0
11	0.0	0.0	0.0	0.0017152070254879764	0.0
12	0.0	0.0	0.0	0.0017152070254879764	0.0
13	0.0	0.0	0.0	0.0017152070254879764	0.0
14	0.0	0.0	0.0	0.0017152070254879764	0.0
15	0.0	0.0	0.0	0.0025728105382319647	0.0
16	0.0	0.0	0.0	0.0025728105382319647	0.0
17	0.0	0.0	0.0	0.0025728105382319647	0.0
18	0.0	0.0	0.0	0.0034304140509759527	0.0
19	0.0	0.0	0.0	0.0034304140509759527	0.0
20	0.0	0.0	0.0	0.004288017563719941	0.0
21	0.0	0.0	0.0	0.005145621076463929	0.0
22	0.0	0.0	0.0	0.005145621076463929	0.0
23	0.0	0.0	0.0	0.006003224589207917	0.0
24	0.0	0.0	0.0	0.007718431614695894	0.0
25	0.0	0.0	0.0	0.008576035127439883	0.0
26	0.0	0.0	0.0	0.00943363864018387	0.0
27	0.0	0.0	0.0	0.029158519433295598	0.0
28	0.0	0.0	0.0	0.04631058968817536	0.0
29	0.0	0.0	0.0	0.07118109155775101	0.0
30	0.0	0.0	0.0	0.09690919694007066	0.0
31	0.0	0.0	0.0	0.11749168124592638	0.0
32	0.0	0.0	0.0	0.1397893725772701	0.0
33	0.0	0.0	0.0	0.18266954821446948	0.0
34	0.0	0.0	0.0	0.27443312407807624	0.0
35	0.0	0.0	0.0	0.41765291070632227	0.0
36	0.0	0.0	0.0	0.6200473397139035	0.0
37	0.0	0.0	0.0	0.945079071043875	0.0
38	0.0	0.0	0.0	1.385029673081541	0.0
39	0.0	0.0	0.0	1.893588556138726	0.0
40	0.0	0.0	0.0	2.47075572021543	0.0
41	0.0	0.0	0.0	3.134540839079277	0.0
42	0.0	0.0	0.0	3.904668793523378	0.0
43	0.0	0.0	0.0	4.776851565984014	0.0
44	0.0	0.0	0.0	5.6901993070563615	0.0
45	0.0	0.0	0.0	6.781070975266715	0.0
46	0.0	0.0	0.0	7.881376282117252	0.0
47	0.0	0.0	0.0	9.130904600185243	0.0
48	0.0	0.0	0.0	10.31525505128469	0.0
49	0.0	0.0	0.0	11.341806456039244	0.0
50	0.0	0.0	0.0	12.27230626736647	0.0
51	0.0	0.0	0.0	13.249974271894617	0.0
52	0.0	0.0	0.0	14.168467634043429	0.0
53	0.0	0.0	0.0	15.039792802991322	0.0
54	0.0	0.0	0.0	16.021748825083186	0.0
55	0.0	0.0	0.0	17.11690851085726	0.0
56	0.0	0.0	0.0	18.092861308359918	0.0
57	0.0	0.0	0.0	19.154574457136977	0.0
58	0.0	0.0	0.0	20.079071043874997	0.0
59	0.0	0.0	0.0	21.010428458714966	0.0
60	0.0	0.0	0.0	21.83287022743645	0.0
61	0.0	0.0	0.0	22.732496312304896	0.0
62	0.0	0.0	0.0	23.554080477513637	0.0
63	0.0	0.0	0.0	24.30362594765188	0.0
64	0.0	0.0	0.0	25.106342835580254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	15	0.0022198802	70.0	8
CCATTCG	15	0.0022198802	70.0	67
CTTGAAA	490	0.0	55.0	57
TCGTATG	525	0.0	54.666668	40
CGTATGC	530	0.0	54.150944	41
CTCGTAT	530	0.0	54.150944	39
GCCTTAT	485	0.0	54.12371	32
TATGCCG	525	0.0	54.0	43
ATCTCGT	490	0.0	53.57143	37
CCGTCTT	500	0.0	53.199997	47
TGCCTTA	540	0.0	53.148148	31
CGTCTTC	495	0.0	53.030304	48
TCTCGTA	495	0.0	53.030304	38
GCCGTCT	515	0.0	53.009712	46
TGCCGTC	525	0.0	52.666668	45
GACAGTG	20	0.0069364584	52.500004	68
ATCAACG	20	0.0069364584	52.500004	7
TACGAGT	20	0.0069364584	52.500004	44
GCGTGCG	20	0.0069364584	52.500004	23
CGTCCGT	20	0.0069364584	52.500004	6
>>END_MODULE