FastQCFastQC Report
Wed 25 May 2016
SRR1781165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85105
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG150.002217812570.0000136
GGTAGTG150.002217812570.0000125
ATAGCGG252.378434E-456.00000437
GCGGTAA200.006930047652.551
TAGCGGC200.006930047652.538
TCGATCC200.006930047652.522
TTCTTAG200.006930047652.528
ACATCGA200.006930047652.570
CAGTAAG200.006930047652.511
CGGTAAC200.006930047652.552
CTTAGGT200.006930047652.530
GTTATAT200.006930047652.53
TGGTAAT200.006930047652.570
ACTTATC352.0710662E-550.08
TACTGAC506.111077E-849.00000434
CTACTGA551.2935197E-744.54545633
TCTACAC404.558862E-543.753
CGTAGGA404.558862E-543.752
GACTATC404.558862E-543.7538
GAAAAAC350.001249228840.025
Produced by FastQC (version 0.11.5)