##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781165_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85105
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.692344750602196	34.0	32.0	34.0	21.0	34.0
2	30.878256271664416	34.0	32.0	34.0	21.0	34.0
3	30.762881146818636	34.0	32.0	34.0	21.0	34.0
4	30.957957816814524	34.0	32.0	34.0	21.0	34.0
5	30.973515069619882	34.0	32.0	34.0	21.0	34.0
6	33.60257329181599	37.0	34.0	38.0	21.0	38.0
7	33.56375066094824	37.0	34.0	38.0	21.0	38.0
8	33.38370248516539	37.0	34.0	38.0	21.0	38.0
9	33.679466541331294	37.0	34.0	38.0	21.0	38.0
10-11	33.52013982727219	37.0	34.0	38.0	21.0	38.0
12-13	33.46329827859702	37.0	34.0	38.0	21.0	38.0
14-15	33.308977145878615	37.0	34.0	38.0	21.0	38.0
16-17	33.259790846601256	37.0	34.0	38.0	21.0	38.0
18-19	33.29027671699665	37.0	34.0	38.0	21.0	38.0
20-21	33.23809999412491	37.0	34.0	38.0	21.0	38.0
22-23	33.2985958521826	37.0	34.0	38.0	21.0	38.0
24-25	33.27253392867634	37.0	34.0	38.0	21.0	38.0
26-27	33.18359085835145	37.0	34.0	38.0	21.0	38.0
28-29	33.2489219199812	37.0	34.0	38.0	21.0	38.0
30-31	33.33909288525938	37.0	34.0	38.0	21.0	38.0
32-33	33.2965571940544	37.0	34.0	38.0	21.0	38.0
34-35	33.298454849891314	37.0	34.0	38.0	21.0	38.0
36-37	33.14269431878268	37.0	34.0	38.0	21.0	38.0
38-39	33.174848716291635	37.0	34.0	38.0	21.0	38.0
40-41	33.153569120498204	37.0	34.0	38.0	21.0	38.0
42-43	33.20613359967099	37.0	34.0	38.0	21.0	38.0
44-45	33.28045355737031	37.0	34.0	38.0	21.0	38.0
46-47	33.23284765877446	37.0	34.0	38.0	21.0	38.0
48-49	33.202232536278714	37.0	34.0	38.0	21.0	38.0
50-51	33.05528464837553	37.0	33.5	38.0	21.0	38.0
52-53	33.13767111215557	37.0	34.0	38.0	21.0	38.0
54-55	33.11834204805828	37.0	34.0	38.0	21.0	38.0
56-57	33.07322718994183	37.0	33.5	38.0	21.0	38.0
58-59	33.09138123494507	37.0	34.0	38.0	21.0	38.0
60-61	33.05265260560484	37.0	33.5	38.0	21.0	38.0
62-63	33.00736736971976	37.0	33.0	38.0	21.0	38.0
64-65	33.01428823218377	37.0	33.0	38.0	21.0	38.0
66-67	32.886575406850355	37.0	32.0	38.0	21.0	38.0
68-69	32.66115386875037	37.0	31.5	38.0	15.0	38.0
70-71	32.811526937312735	37.0	32.0	38.0	19.5	38.0
72-73	32.786493155513774	37.0	32.0	38.0	19.5	38.0
74-75	32.78919569943011	37.0	31.5	38.0	19.5	38.0
76	31.31562187885553	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	27.0
14	89.0
15	266.0
16	598.0
17	1079.0
18	1425.0
19	1397.0
20	1209.0
21	994.0
22	994.0
23	1029.0
24	1090.0
25	1256.0
26	1417.0
27	1655.0
28	1942.0
29	2290.0
30	2860.0
31	3373.0
32	3978.0
33	4942.0
34	6120.0
35	7979.0
36	11979.0
37	25113.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.18418424299394	17.63468656365666	10.269666882086833	26.911462311262557
2	23.69896010810176	19.481816579519418	30.9805534339933	25.838669878385524
3	21.06926737559485	20.689736208213382	27.732800658010692	30.508195758181074
4	15.885083132600904	15.237647611773692	33.46571881793079	35.41155043769461
5	15.78050643322954	32.08389636331591	33.84289994712414	18.292697256330413
6	32.278949532929914	33.45396862699019	17.20815463251278	17.058927207567123
7	30.517595910933554	26.44380471182657	21.26549556430292	21.773103812936963
8	26.94906292227249	32.06744609599906	18.896657070677396	22.086833911051055
9	25.140708536513717	14.878091768991247	20.895364549673932	39.085835144821104
10-11	27.573585570765523	22.7037189354327	25.185946771635038	24.536748722166735
12-13	26.415604253569118	20.98231596263439	24.687738675753483	27.914341108043004
14-15	24.94976793372892	22.182597967216967	22.267199341989308	30.600434757064804
16-17	25.711767816227017	24.190118089418952	23.41636801598026	26.681746078373774
18-19	24.98090593972152	23.703660184477997	24.439809646906763	26.87562422889372
20-21	25.136595969684507	24.18306797485459	24.232418776805122	26.44791727865578
22-23	25.831032254274135	23.66899712120322	24.24534398683979	26.25462663768286
24-25	25.22060983490982	23.443980964690674	24.370483520357205	26.964925680042303
26-27	24.59373714822866	24.09670407144116	24.832266024322898	26.477292756007287
28-29	25.36572469302626	23.788261559250337	25.062569766758706	25.783443980964694
30-31	25.532577404382824	23.295928558839083	24.542036308090008	26.629457728688088
32-33	24.839903648434287	23.912813583220725	24.65013806474355	26.597144703601433
34-35	25.885083132600904	23.25656541918806	24.839903648434287	26.018447799776744
36-37	25.925621291345983	22.809470653898124	24.497973092062747	26.766934962693146
38-39	24.802890546971387	23.357029551730214	25.561952881734328	26.278127019564067
40-41	25.352211973444565	22.664943305328713	26.007285118383173	25.975559602843546
42-43	24.411021679102284	22.757769813759474	25.670054638387874	27.161153868750365
44-45	23.489219199812	23.21073967451971	26.608894894542033	26.691146231126258
46-47	24.08730391868868	22.764819928323835	26.58656953175489	26.561306621232596
48-49	23.46219376064861	22.87938428999471	26.852123847012514	26.80629810234416
50-51	23.242465190059338	23.260090476470243	26.316315140121027	27.181129193349392
52-53	22.918159920098702	23.221902355913286	27.070677398507726	26.789260325480292
54-55	22.429939486516655	23.4580811938194	26.791022854121376	27.32095646554257
56-57	22.540978790905353	23.721872980435933	26.734034428059456	27.00311380059926
58-59	22.83179601668527	23.316491392985135	26.6218201045767	27.22989248575289
60-61	22.415839257387933	23.153163738910756	26.64532048645791	27.785676517243406
62-63	22.318312672580927	22.98924857528935	26.772222548616416	27.92021620351331
64-65	22.89994712414077	23.092650255566653	26.21115093120263	27.796251689089946
66-67	22.87233417543035	23.156101286645907	25.593678397273955	28.37788614064978
68-69	22.63145526114799	23.42988073556195	25.683567357969565	28.25509664532049
70-71	23.217789789084073	22.636155337524237	26.5049057047177	27.64114916867399
72-73	23.970389518829684	22.290112214323482	25.811056929675107	27.928441337171726
74-75	23.659009458903707	22.904647200517008	26.008460137477236	27.42788320310205
76	24.00681511074555	22.69666882086834	25.6588919569943	27.637624111391812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	3.0
15	6.5
16	13.5
17	20.0
18	24.0
19	30.5
20	42.5
21	52.0
22	61.5
23	98.5
24	155.0
25	184.0
26	225.0
27	330.5
28	467.5
29	540.0
30	624.5
31	745.5
32	956.0
33	1130.0
34	1268.5
35	1642.5
36	2108.0
37	2338.0
38	2509.5
39	3077.0
40	3638.0
41	3957.5
42	4112.0
43	4322.0
44	4472.5
45	4296.5
46	4180.0
47	4158.0
48	3943.5
49	3552.5
50	3354.0
51	3138.5
52	2655.0
53	2279.0
54	2171.0
55	2074.0
56	1846.0
57	1640.0
58	1565.0
59	1523.0
60	1437.0
61	1416.5
62	1440.0
63	1465.5
64	1466.5
65	1487.5
66	1571.0
67	1609.0
68	1612.0
69	1597.0
70	1584.0
71	1589.0
72	1589.5
73	1515.0
74	1372.5
75	1305.0
76	1208.0
77	1042.5
78	865.0
79	756.0
80	651.5
81	492.0
82	359.0
83	281.0
84	213.0
85	117.5
86	70.5
87	51.0
88	39.0
89	19.0
90	10.5
91	6.0
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	85105.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.01974032078022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5487849643932	48.36613594970918
2	13.242419792966743	16.95552552728982
3	5.137288011159239	9.866635332824158
4	2.184127450260627	5.593090887726926
5	1.1875045885030469	3.801186769285001
6	0.7855517216063432	3.017449033546795
7	0.48638132295719844	2.1796604194818165
8	0.3579032376477498	1.8330297867340344
9	0.2863225901181998	1.6497268080606309
>10	0.7837163203876366	6.737559485341636
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011750190940602785	0.0
2	0.0	0.0	0.0	0.0011750190940602785	0.0
3	0.0	0.0	0.0	0.0011750190940602785	0.0
4	0.0	0.0	0.0	0.004700076376241114	0.0
5	0.0	0.0	0.0	0.004700076376241114	0.0
6	0.0	0.0	0.0	0.004700076376241114	0.0
7	0.0	0.0	0.0	0.004700076376241114	0.0
8	0.0	0.0	0.0	0.005875095470301393	0.0
9	0.0	0.0	0.0	0.009400152752482228	0.0
10	0.0	0.0	0.0	0.010575171846542506	0.0
11	0.0	0.0	0.0	0.010575171846542506	0.0
12	0.0	0.0	0.0	0.011750190940602786	0.0
13	0.0	0.0	0.0	0.012925210034663064	0.0
14	0.0	0.0	0.0	0.012925210034663064	0.0
15	0.0	0.0	0.0	0.012925210034663064	0.0
16	0.0	0.0	0.0	0.012925210034663064	0.0
17	0.0	0.0	0.0	0.012925210034663064	0.0
18	0.0	0.0	0.0	0.0164502673168439	0.0
19	0.0	0.0	0.0	0.0164502673168439	0.0
20	0.0	0.0	0.0	0.017625286410904176	0.0
21	0.0	0.0	0.0	0.017625286410904176	0.0
22	0.0	0.0	0.0	0.02115034369308501	0.0
23	0.0	0.0	0.0	0.02115034369308501	0.0
24	0.0	0.0	0.0	0.02232536278714529	0.0
25	0.0	0.0	0.0	0.02350038188120557	0.0
26	0.0	0.0	0.0	0.024675400975265847	0.0
27	0.0	0.0	0.0	0.03877563010398919	0.0
28	0.0	0.0	0.0	0.06815110745549616	0.0
29	0.0	0.0	0.0	0.09635156571294283	0.0
30	0.0	0.0	0.0	0.12102696668820868	0.0
31	0.0	0.0	0.0	0.16685271135655955	0.0
32	0.0	0.0	0.0	0.21150343693085014	0.0
33	0.0	0.0	0.0	0.25850420069326124	0.0
34	0.0	0.0	0.0	0.35955584278244523	0.0
35	0.0	0.0	0.0	0.527583573233065	0.0
36	0.0	0.0	0.0	0.7625873920451207	0.0
37	0.0	0.0	0.0	1.1879443040949416	0.0
38	0.0	0.0	0.0	1.6485517889665706	0.0
39	0.0	0.0	0.0	2.100934140179778	0.0
40	0.0	0.0	0.0	2.5803419305563717	0.0
41	0.0	0.0	0.0	3.042124434522061	0.0
42	0.0	0.0	0.0	3.530932377651137	0.0
43	0.0	0.0	0.0	4.143117325656542	0.0
44	0.0	0.0	0.0	4.785852770107514	0.0
45	0.0	0.0	0.0	5.573115563127901	0.0
46	0.0	0.0	0.0	6.28400211503437	0.0
47	0.0	0.0	0.0	7.114740614534986	0.0
48	0.0	0.0	0.0	7.852652605604841	0.0
49	0.0	0.0	0.0	8.57058927207567	0.0
50	0.0	0.0	0.0	9.183949239175137	0.0
51	0.0	0.0	0.0	9.919511192056872	0.0
52	0.0	0.0	0.0	10.496445567240468	0.0
53	0.0	0.0	0.0	11.195581928206334	0.0
54	0.0	0.0	0.0	11.870042888196933	0.0
55	0.0	0.0	0.0	12.609129898360848	0.0
56	0.0	0.0	0.0	13.324716526643558	0.0
57	0.0	0.0	0.0	14.035603078550027	0.0
58	0.0	0.0	0.0	14.697138828505963	0.0
59	0.0	0.0	0.0	15.289348451912344	0.0
60	0.0	0.0	0.0	15.919158686328652	0.0
61	0.0	0.0	0.0	16.52664355795782	0.0
62	0.0	0.0	0.0	17.109453028611714	0.0
63	0.0	0.0	0.0	17.76276364490923	0.0
64	0.0	0.0	0.0	18.337347981904706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAGCG	15	0.0022178125	70.00001	36
GGTAGTG	15	0.0022178125	70.00001	25
ATAGCGG	25	2.378434E-4	56.000004	37
GCGGTAA	20	0.0069300476	52.5	51
TAGCGGC	20	0.0069300476	52.5	38
TCGATCC	20	0.0069300476	52.5	22
TTCTTAG	20	0.0069300476	52.5	28
ACATCGA	20	0.0069300476	52.5	70
CAGTAAG	20	0.0069300476	52.5	11
CGGTAAC	20	0.0069300476	52.5	52
CTTAGGT	20	0.0069300476	52.5	30
GTTATAT	20	0.0069300476	52.5	3
TGGTAAT	20	0.0069300476	52.5	70
ACTTATC	35	2.0710662E-5	50.0	8
TACTGAC	50	6.111077E-8	49.000004	34
CTACTGA	55	1.2935197E-7	44.545456	33
TCTACAC	40	4.558862E-5	43.75	3
CGTAGGA	40	4.558862E-5	43.75	2
GACTATC	40	4.558862E-5	43.75	38
GAAAAAC	35	0.0012492288	40.0	25
>>END_MODULE