FastQCFastQC Report
Wed 25 May 2016
SRR1781165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77230
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT37664.8763433898744015No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3530.45707626569985754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2530.32759290431179594No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAACCTCGTATGCCGT2150.27838922698433255No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAAACTCGTATGCCGT1570.20328887737925677No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT850.1100608571798524RNA PCR Primer, Index 32 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCATC150.002217029570.000018
CGCAGTG150.002217029570.0000114
GATTATA150.002217029570.0000120
CCCGCTA150.002217029570.0000142
TCGTTGT207.881937E-569.9999934
ATCTCGT5350.056.2616837
TCTCGTA5350.056.2616838
ATCGTTG252.3770437E-456.00000433
TTTCAAC252.3770437E-456.00000442
CATCGTT252.3770437E-456.00000432
TATGCCG6100.055.6557443
AATCTCG5350.055.60747536
CGTATGC6000.055.41666841
CTTGAAA5500.055.36363257
GTATGCC6100.055.0819742
TCGTATG6000.054.83333240
GCCGTCT6000.054.83333246
CCGTCTT5900.054.5762747
CTCGTAT5850.054.4444539
TTGAAAA5600.054.37558