FastQCFastQC Report
Wed 25 May 2016
SRR1781164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811020
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA350.001255550240.01
GATAACG450.004307721731.11110944
TCTACGC500.007209136628.0000023
TACACCG755.586069E-528.0000025
GGTATAG2900.024.137931
TGGTACG1601.8626451E-924.062570
GCCCGCA952.7804292E-422.10526313
TCGGTGT850.00378981620.58823634
TTCGATG1257.822737E-519.647
CGTCAGC900.00527555219.44444365
CGATTGG8750.018.869
GTCCATA1702.1182113E-618.5294111
GTTTGCG1000.00968798317.50000238
TTTCGAC1000.00968798317.50000228
GCGTTTG1000.00968798317.50000221
GGTATAC2005.145903E-717.5000021
TTTGCGG1000.00968798317.50000239
GCTCTAG2207.26468E-817.51
GCTCTAC2056.6644134E-717.0731721
TATAGTG3307.2759576E-1216.9696965
Produced by FastQC (version 0.11.5)