##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781164_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	811020
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566868881161994	34.0	33.0	34.0	24.0	34.0
2	31.69925895785554	34.0	33.0	34.0	24.0	34.0
3	31.643684496066683	34.0	34.0	34.0	23.0	34.0
4	31.84566841754827	34.0	34.0	34.0	27.0	34.0
5	31.928845157949247	34.0	34.0	34.0	27.0	34.0
6	34.84416537200069	38.0	35.0	38.0	27.0	38.0
7	34.67916450889004	38.0	35.0	38.0	26.0	38.0
8	34.45293087716703	37.0	34.0	38.0	26.0	38.0
9	34.776041281349414	38.0	35.0	38.0	27.0	38.0
10-11	34.67541306009716	38.0	35.0	38.0	26.5	38.0
12-13	34.624824295331806	38.0	35.0	38.0	26.0	38.0
14-15	34.52662696357673	38.0	34.0	38.0	26.0	38.0
16-17	34.52004266232645	38.0	34.5	38.0	26.0	38.0
18-19	34.58531910433775	38.0	34.5	38.0	26.0	38.0
20-21	34.51924120243644	37.5	34.5	38.0	26.0	38.0
22-23	34.58910384454144	38.0	34.5	38.0	26.5	38.0
24-25	34.59721955068925	38.0	34.5	38.0	26.0	38.0
26-27	34.524405070158565	38.0	34.0	38.0	26.0	38.0
28-29	34.615960765455846	38.0	35.0	38.0	26.0	38.0
30-31	34.69468878695963	38.0	35.0	38.0	27.0	38.0
32-33	34.6559536139676	38.0	35.0	38.0	26.5	38.0
34-35	34.68450654731079	38.0	35.0	38.0	26.5	38.0
36-37	34.53751757046682	38.0	34.5	38.0	26.0	38.0
38-39	34.57341187640256	38.0	34.5	38.0	26.0	38.0
40-41	34.55020344751054	37.5	34.5	38.0	26.0	38.0
42-43	34.630334640329465	38.0	35.0	38.0	26.5	38.0
44-45	34.631820423664024	38.0	35.0	38.0	26.5	38.0
46-47	34.61985154496807	38.0	35.0	38.0	26.5	38.0
48-49	34.589079184237136	37.5	34.5	38.0	26.0	38.0
50-51	34.47595558679194	37.0	34.0	38.0	25.5	38.0
52-53	34.55466942862075	37.0	34.0	38.0	26.0	38.0
54-55	34.51405020837957	37.0	34.0	38.0	26.0	38.0
56-57	34.477356908584255	37.0	34.0	38.0	25.5	38.0
58-59	34.4836021306503	37.0	34.0	38.0	25.5	38.0
60-61	34.46861729673744	37.0	34.0	38.0	25.5	38.0
62-63	34.45109245148085	37.0	34.0	38.0	25.0	38.0
64-65	34.46387142117334	37.0	34.0	38.0	25.0	38.0
66-67	34.3672375527114	37.0	34.0	38.0	25.0	38.0
68-69	34.17971258415329	37.0	34.0	38.0	24.5	38.0
70-71	34.345226998101154	37.0	34.0	38.0	25.0	38.0
72-73	34.33587211166186	37.0	34.0	38.0	24.5	38.0
74-75	34.359664989765974	37.0	34.0	38.0	25.0	38.0
76	33.1652610293211	37.0	32.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	22.0
14	92.0
15	335.0
16	729.0
17	1430.0
18	2221.0
19	3001.0
20	3971.0
21	4800.0
22	5879.0
23	7299.0
24	8814.0
25	10587.0
26	12736.0
27	15113.0
28	18282.0
29	21902.0
30	26033.0
31	31561.0
32	38078.0
33	47270.0
34	60591.0
35	81201.0
36	124816.0
37	284248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.0931419693719	19.081526966042762	9.177208946758403	23.648122117826933
2	21.95186308599048	22.6093067988459	32.70634509629849	22.732485018865134
3	18.841952109689032	23.853419151192327	31.68812113141476	25.61650760770388
4	13.199304579418511	17.738156888856007	39.0816502675643	29.98088826416118
5	12.77206480727972	34.40544006313038	37.48427905600355	15.338216073586347
6	27.80202707701413	39.17794875588765	20.023427289092748	12.996596878005477
7	26.278266873813223	32.058518902123254	23.327044955734756	18.336169268328774
8	23.665507632364182	37.27898202263816	21.501196024758944	17.554314320238714
9	24.023698552440138	17.79401247811398	22.882296367537176	35.299992601908706
10-11	23.526916722152354	26.02346427954921	29.519863875120215	20.92975512317822
12-13	24.088863406574436	24.460247589455253	28.768587704372273	22.682301299598038
14-15	22.110798747256542	25.427054819856476	26.004969051318096	26.457177381568886
16-17	21.963700032058394	29.0275208996079	26.60224162166161	22.406537446672093
18-19	21.1442381198984	28.65891100096175	28.42352839634041	21.773322482799436
20-21	21.456375921678873	28.81889472516091	28.329326033883255	21.39540331927696
22-23	21.32678602253952	28.600712682794505	28.512243841088996	21.560257453576977
24-25	21.537015116766543	28.299055510345	28.237713003378463	21.926216369509998
26-27	21.218403985105176	28.18376858770437	28.423836650144263	22.17399077704619
28-29	21.212978718157384	28.263852925945105	28.769512465783826	21.753655890113684
30-31	21.30138590910212	28.633079332198957	28.495721437202537	21.569813321496387
32-33	20.872728169465613	28.55848191166679	28.960938077975882	21.60785184089172
34-35	21.369448348992627	28.178096717713498	28.963095854602845	21.48935907869103
36-37	21.62394268945279	28.406019580281622	28.269586446696753	21.70045128356884
38-39	21.266861483070702	28.43363912110675	28.688071810806143	21.6114275850164
40-41	21.140045868166013	28.230684816650637	28.99885329584967	21.63041601933368
42-43	21.054721215259796	28.10047840990358	29.081095410717367	21.763704964119256
44-45	20.952504253902493	27.890619220241174	29.186703163917045	21.970173361939285
46-47	21.095472368129023	28.09906044240586	29.023451949397057	21.782015240068063
48-49	20.67347291065572	27.879028877216346	29.38404724914305	22.063450962984884
50-51	20.74233681043624	27.78945032181697	29.38682153337772	22.081391334369066
52-53	20.379213829498653	27.72003156518951	29.668627160859163	22.232127444452665
54-55	20.23562920766442	27.828351951863084	29.668133954773	22.26788488569949
56-57	20.290831657458085	27.59781213510411	29.69535855510167	22.415997652336138
58-59	20.233792159128107	27.511299050146143	29.745215743887787	22.50969304683796
60-61	20.23029053573344	27.75890577162804	29.319596704886074	22.691206987752444
62-63	20.058457287401843	27.659815824403726	29.472780864085262	22.80894602410916
64-65	20.10019487817494	27.407047549411573	29.443743337860557	23.04901423455293
66-67	20.039468841378046	27.304460620244026	29.300528532297932	23.35554200608
68-69	20.34610737096496	27.072082069492737	29.15439816527336	23.427412394268945
70-71	20.87588522840696	27.205079532612963	28.771503027978984	23.147532211001092
72-73	20.601156568272053	26.92473675125151	29.028507311780228	23.44559936869621
74-75	20.880187911518828	26.73559221720796	28.959520110478167	23.424699760795047
76	20.882838894231956	26.78281669995808	28.756750758304356	23.57759364750561
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.0
8	3.0
9	3.0
10	6.5
11	17.5
12	25.0
13	29.0
14	71.0
15	167.0
16	386.5
17	548.0
18	737.0
19	1290.5
20	2117.0
21	2579.0
22	3279.0
23	4900.0
24	6897.0
25	7973.0
26	9144.5
27	11612.0
28	14325.0
29	15742.0
30	17390.5
31	20779.5
32	24375.5
33	26231.0
34	27927.0
35	31606.5
36	35062.5
37	36535.0
38	37986.0
39	40893.5
40	42854.0
41	43258.0
42	43158.0
43	42646.5
44	41692.5
45	40065.5
46	38881.0
47	37375.0
48	34485.0
49	31532.0
50	29963.0
51	28231.5
52	24879.0
53	22301.0
54	21344.0
55	20226.5
56	17996.5
57	15479.0
58	14074.0
59	12973.0
60	10703.5
61	8710.0
62	7885.0
63	7094.0
64	5690.5
65	4739.0
66	4054.5
67	3709.0
68	3512.0
69	3164.0
70	2833.0
71	2653.0
72	2481.5
73	2159.0
74	1883.5
75	1759.0
76	1586.0
77	1309.0
78	1100.0
79	995.0
80	878.0
81	673.5
82	498.0
83	410.0
84	353.5
85	229.0
86	138.0
87	115.0
88	87.0
89	48.0
90	32.0
91	19.0
92	11.0
93	11.0
94	7.0
95	3.0
96	3.0
97	3.0
98	4.5
99	8.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.2330152154077582E-4
58-59	6.165076077038791E-5
60-61	1.2330152154077582E-4
62-63	6.165076077038791E-5
64-65	6.165076077038791E-5
66-67	6.165076077038791E-5
68-69	0.0
70-71	6.165076077038791E-5
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	811020.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.67123659639465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39632717767489	48.267526579727594
2	18.190193674115697	24.25525412362418
3	5.919733691359727	11.84027896572879
4	1.774576056022219	4.73252720357417
5	0.5792790982054657	1.931062690590137
6	0.26116951921467607	1.0447496884396985
7	0.16444441615132496	0.7674598803326678
8	0.11118211716957807	0.5930119391280809
9	0.09111229022408279	0.5467112152532279
>10	0.5036338651966009	5.696506617274945
>50	0.008348094665710876	0.32491109632651577
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.932060861631033E-4	0.0
2	0.0	0.0	0.0	4.932060861631033E-4	0.0
3	0.0	0.0	0.0	4.932060861631033E-4	0.0
4	0.0	0.0	0.0	8.631106507854307E-4	0.0
5	0.0	0.0	0.0	0.001233015215407758	0.0
6	0.0	0.0	0.0	0.0013563167369485339	0.0
7	0.0	0.0	0.0	0.0014796182584893099	0.0
8	0.0	0.0	0.0	0.0014796182584893099	0.0
9	0.0	0.0	0.0	0.0022194273877339647	0.0
10	0.0	0.0	0.0	0.002589331952356292	0.0
11	0.0	0.0	0.0	0.002589331952356292	0.0
12	0.0	0.0	0.0	0.0027126334738970677	0.0
13	0.0	0.0	0.0	0.0030825380385193953	0.0
14	0.0	0.0	0.0	0.0030825380385193953	0.0
15	0.0	0.0	0.0	0.0032058395600601712	0.0
16	0.0	0.0	0.0	0.003329141081600947	0.0
17	0.0	0.0	0.0	0.0034524426031417228	0.0
18	0.0	0.0	0.0	0.0036990456462232743	0.0
19	0.0	0.0	0.0	0.0038223471677640503	0.0
20	0.0	0.0	0.0	0.003945648689304826	0.0
21	0.0	0.0	0.0	0.004192251732386377	0.0
22	0.0	0.0	0.0	0.004685457818549481	0.0
23	0.0	0.0	0.0	0.004808759340090256	0.0
24	0.0	0.0	0.0	0.0054252669477941355	0.0
25	0.0	0.0	0.0	0.005918473033957239	0.0
26	0.0	0.0	0.0	0.0064116791201203425	0.0
27	0.0	0.0	0.0	0.008877709550935859	0.0
28	0.0	0.0	0.0	0.01627580084338241	0.0
29	0.0	0.0	0.0	0.025400113437399816	0.0
30	0.0	0.0	0.0	0.03612734581144731	0.0
31	0.0	0.0	0.0	0.048827402530147224	0.0
32	0.0	0.0	0.0	0.06054104707652092	0.0
33	0.0	0.0	0.0	0.07669354639836255	0.0
34	0.0	0.0	0.0	0.09876451875416142	0.0
35	0.0	0.0	0.0	0.12737047175162142	0.0
36	0.0	0.0	0.0	0.1805134275356958	0.0
37	0.0	0.0	0.0	0.27607210672979704	0.0
38	0.0	0.0	0.0	0.43463786343123473	0.0
39	0.0	0.0	0.0	0.6531281596014895	0.0
40	0.0	0.0	0.0	0.9100885304924663	0.0
41	0.0	0.0	0.0	1.160513920741782	0.0
42	0.0	0.0	0.0	1.403664521220192	0.0
43	0.0	0.0	0.0	1.674188059480654	0.0
44	0.0	0.0	0.0	1.968385489876945	0.0
45	0.0	0.0	0.0	2.3062316588986707	0.0
46	0.0	0.0	0.0	2.69228872284284	0.0
47	0.0	0.0	0.0	3.1126236097753446	0.0
48	0.0	0.0	0.0	3.611131661364701	0.0
49	0.0	0.0	0.0	4.142931123770067	0.0
50	0.0	0.0	0.0	4.697911272225099	0.0
51	0.0	0.0	0.0	5.202584399891495	0.0
52	0.0	0.0	0.0	5.693077852580701	0.0
53	0.0	0.0	0.0	6.118468101896378	0.0
54	0.0	0.0	0.0	6.529432073191783	0.0
55	0.0	0.0	0.0	6.966659268575374	0.0
56	0.0	0.0	0.0	7.413503982639146	0.0
57	0.0	0.0	0.0	7.842469976079505	0.0
58	0.0	0.0	0.0	8.34097802766886	0.0
59	0.0	0.0	0.0	8.83517052600429	0.0
60	0.0	0.0	0.0	9.372395255357452	0.0
61	0.0	0.0	0.0	9.880644127148528	0.0
62	0.0	0.0	0.0	10.336119947720155	0.0
63	0.0	0.0	0.0	10.77729279179305	0.0
64	0.0	0.0	0.0	11.192449014820843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	35	0.0012555502	40.0	1
GATAACG	45	0.0043077217	31.111109	44
TCTACGC	50	0.0072091366	28.000002	3
TACACCG	75	5.586069E-5	28.000002	5
GGTATAG	290	0.0	24.13793	1
TGGTACG	160	1.8626451E-9	24.0625	70
GCCCGCA	95	2.7804292E-4	22.105263	13
TCGGTGT	85	0.003789816	20.588236	34
TTCGATG	125	7.822737E-5	19.6	47
CGTCAGC	90	0.005275552	19.444443	65
CGATTGG	875	0.0	18.8	69
GTCCATA	170	2.1182113E-6	18.529411	1
GTTTGCG	100	0.009687983	17.500002	38
TTTCGAC	100	0.009687983	17.500002	28
GCGTTTG	100	0.009687983	17.500002	21
GGTATAC	200	5.145903E-7	17.500002	1
TTTGCGG	100	0.009687983	17.500002	39
GCTCTAG	220	7.26468E-8	17.5	1
GCTCTAC	205	6.6644134E-7	17.073172	1
TATAGTG	330	7.2759576E-12	16.969696	5
>>END_MODULE