FastQCFastQC Report
Wed 25 May 2016
SRR1781164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731625
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT23800.3253032632837861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT4650.046.66666847
TATGCCG4800.046.66666443
CTCGTAT4600.046.4130439
GCCGTCT4800.045.937546
TCTCGTA4350.045.05747238
CGTCTTC4950.044.54545248
ATCTCGT4550.043.07692337
TCGTATG5200.043.07692340
ATGCCGT5200.043.07692344
CGTATGC5050.042.970341
TGCCGTC5150.042.81553345
TATCTCG4700.040.95744736
GCACGAT350.001255469540.016
GTATGCC6450.035.27131742
AGGACCG400.002418860635.030
TTCGTAA450.00430744831.11111320
GTAAGCG450.00430744831.11111346
CGGTCAT7000.031.00000228
CTGCTTG7500.029.40000254
ACTACGC604.982543E-429.1666668
Produced by FastQC (version 0.11.5)