##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781164_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731625
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25217563642576	34.0	34.0	34.0	32.0	34.0
2	33.32138458909961	34.0	34.0	34.0	33.0	34.0
3	33.3825675721852	34.0	34.0	34.0	33.0	34.0
4	33.45473432427815	34.0	34.0	34.0	34.0	34.0
5	33.437616265163165	34.0	34.0	34.0	34.0	34.0
6	36.85382948915086	38.0	37.0	38.0	34.0	38.0
7	36.79571228429865	38.0	37.0	38.0	34.0	38.0
8	36.58990056381343	38.0	37.0	38.0	34.0	38.0
9	36.54962856654707	38.0	37.0	38.0	34.0	38.0
10-11	36.47674901759781	38.0	37.0	38.0	34.0	38.0
12-13	36.415938493080475	38.0	37.0	38.0	34.0	38.0
14-15	36.4386318127456	38.0	37.0	38.0	34.0	38.0
16-17	36.38740133264992	38.0	37.0	38.0	34.0	38.0
18-19	36.39997266359132	38.0	37.0	38.0	34.0	38.0
20-21	36.33528310268238	38.0	37.0	38.0	34.0	38.0
22-23	36.381427643943276	38.0	37.0	38.0	34.0	38.0
24-25	36.38732615752606	38.0	37.0	38.0	34.0	38.0
26-27	36.184552195455325	38.0	37.0	38.0	34.0	38.0
28-29	36.17036186570989	38.0	37.0	38.0	34.0	38.0
30-31	36.2031792243294	38.0	37.0	38.0	34.0	38.0
32-33	36.165854775328896	38.0	37.0	38.0	34.0	38.0
34-35	36.18170510849137	38.0	37.0	38.0	34.0	38.0
36-37	36.14746557321032	38.0	37.0	38.0	34.0	38.0
38-39	36.15833999658295	38.0	37.0	38.0	34.0	38.0
40-41	36.142732274047496	38.0	37.0	38.0	34.0	38.0
42-43	36.12892465402358	38.0	37.0	38.0	34.0	38.0
44-45	36.10913992824193	38.0	37.0	38.0	33.5	38.0
46-47	36.12295028190672	38.0	37.0	38.0	34.0	38.0
48-49	36.11000239193576	38.0	37.0	38.0	34.0	38.0
50-51	36.0394525884162	38.0	37.0	38.0	32.5	38.0
52-53	36.07403587903639	38.0	37.0	38.0	33.0	38.0
54-55	36.08265504869298	38.0	37.0	38.0	33.5	38.0
56-57	36.01872270630446	38.0	37.0	38.0	32.0	38.0
58-59	36.03721988723731	38.0	37.0	38.0	32.0	38.0
60-61	36.04352776354007	38.0	37.0	38.0	32.5	38.0
62-63	36.02107090381001	38.0	37.0	38.0	32.0	38.0
64-65	35.969691440287036	38.0	37.0	38.0	32.0	38.0
66-67	35.963208610968735	38.0	37.0	38.0	32.0	38.0
68-69	35.931967196309586	38.0	37.0	38.0	32.0	38.0
70-71	35.90343276951991	38.0	37.0	38.0	32.0	38.0
72-73	35.88655390398087	38.0	37.0	38.0	32.0	38.0
74-75	35.88105791901589	38.0	37.0	38.0	32.0	38.0
76	35.10240218691269	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	13.0
21	27.0
22	61.0
23	159.0
24	355.0
25	791.0
26	1505.0
27	2823.0
28	4760.0
29	7866.0
30	12479.0
31	19312.0
32	28410.0
33	40365.0
34	56528.0
35	80479.0
36	128110.0
37	347576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.8316418930463	18.97215103365795	9.627746454809499	24.568460618486245
2	22.22422689219204	23.193029215786776	31.82368016401845	22.759063728002733
3	19.50972151033658	24.191081496668374	31.505484366991286	24.793712626003757
4	13.489560908935589	18.415991799077396	38.54228600717581	29.552161284811206
5	12.756125064069707	34.84572014351615	37.0773278660516	15.32082692636255
6	28.0356740133265	39.52434648898001	19.63847599521613	12.801503502477363
7	26.063625491201094	32.56559029557492	22.988279514778746	18.382504698445242
8	23.620844011617972	37.90944814624979	20.92998462327012	17.53972321886212
9	23.812745600546727	18.12991628224842	22.46492397061336	35.59241414659149
10-11	23.268272680676578	26.48269263625491	29.303604988894584	20.945429694173928
12-13	23.854706987869466	24.757218520416878	28.692499572868613	22.695574918845036
14-15	21.81472749017598	25.603007004954726	25.866666666666667	26.71559883820263
16-17	21.846232701178884	29.252895950794468	26.503604988894587	22.39726635913207
18-19	21.269981206219033	28.787903639159406	28.167367162139072	21.77474799248249
20-21	21.224739449854777	28.866427473090724	28.14850504015035	21.760328036904152
22-23	21.193439261916964	28.868751067828462	28.376217324448998	21.56159234580557
24-25	21.655902955749188	28.686963950111057	28.08276097727661	21.574372116863145
26-27	21.31201093456347	28.408405945668886	28.27910473261575	22.000478387151887
28-29	21.291576969075688	28.35885870493764	28.5662737057919	21.783290620194773
30-31	21.40679993165898	28.54679651460789	28.60556979326841	21.44083376046472
32-33	20.803212028019818	28.85344267896805	28.706031095164875	21.637314197847257
34-35	21.099675380146934	28.25580044421664	29.294105586878523	21.350418588757904
36-37	21.441038783529816	28.62641380488638	28.403690415171706	21.528856996412095
38-39	21.092636254912012	28.52321886212199	28.638988552878864	21.745156330087134
40-41	21.01547924141466	28.07654194430207	29.10322911327524	21.80474970100803
42-43	21.127763540064922	28.039296087476508	29.119835981547926	21.713104390910644
44-45	20.825081154963268	28.22012643089014	29.23997949769349	21.714812916453102
46-47	21.066188279514776	27.952708012984793	29.03885187083547	21.94225183666496
48-49	20.822757560225526	27.81739279002221	29.199521612848113	22.16032803690415
50-51	20.61698274389202	27.69424226892192	29.478216299333678	22.210558687852384
52-53	20.307466256620536	27.60765419443021	30.039774474628395	22.045105074320862
54-55	20.019203827097215	27.801811037075	29.882180078592175	22.296805057235606
56-57	20.1780967025457	27.61619682214249	29.510131556466767	22.695574918845036
58-59	19.843430719289255	27.363676746967368	30.097454296941738	22.69543823680164
60-61	20.146454809499403	27.89154279856484	29.118674184179056	22.843328207756706
62-63	19.650504015035025	28.15123868101828	29.129950452759267	23.068306851187426
64-65	19.582094652315053	28.00416880232359	28.9664103878353	23.447326157526057
66-67	19.527695199043226	27.866324961558174	28.768289765931996	23.837690073466597
68-69	19.854433623782676	27.870357081838375	28.561284811207926	23.713924483171024
70-71	20.33746796514608	27.80379292670425	28.00512557662737	23.853613531522296
72-73	20.200922603792925	27.517785750896977	27.983188108662226	24.298103536647872
74-75	20.21185716726465	27.30770545019648	28.037519220912355	24.44291816162652
76	20.118913377754996	27.45108491371946	27.827780625320347	24.602221083205194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	5.5
12	9.0
13	24.5
14	65.5
15	162.0
16	369.5
17	506.0
18	714.0
19	1239.0
20	2015.5
21	2475.0
22	3132.5
23	4568.0
24	6319.0
25	7292.0
26	8208.0
27	10450.0
28	13249.0
29	14722.0
30	15974.0
31	18816.5
32	22066.0
33	23725.0
34	25343.0
35	28539.5
36	31900.0
37	33682.0
38	34374.0
39	36529.0
40	38646.0
41	39300.5
42	39301.0
43	38676.5
44	37330.5
45	35513.0
46	34417.0
47	33404.0
48	30961.0
49	28139.5
50	26748.0
51	25405.0
52	22569.0
53	19945.0
54	18814.0
55	17953.5
56	16088.0
57	13954.0
58	12825.0
59	11814.5
60	9676.5
61	7784.0
62	7019.0
63	6272.5
64	5073.0
65	4235.0
66	3654.0
67	3458.0
68	3207.0
69	2768.5
70	2459.5
71	2338.0
72	2286.0
73	2082.5
74	1788.5
75	1646.0
76	1512.0
77	1304.5
78	1066.0
79	901.0
80	796.5
81	615.5
82	449.5
83	360.0
84	314.0
85	215.5
86	133.5
87	104.0
88	81.0
89	43.0
90	18.5
91	11.0
92	13.0
93	8.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	731625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.22107109116316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.4426284732257	41.34131509716318
2	22.260096746175314	27.700941242798883
3	7.179462561181584	13.40141551246871
4	2.105433432379453	5.240092930951744
5	0.6922593377447204	2.1536558733667888
6	0.2970236250293859	1.1088676853225046
7	0.19153031029814177	0.8342054737221254
8	0.13254717831476465	0.6597781923884842
9	0.10231862645767341	0.572973707769576
>10	0.5872339840567455	6.25221706176822
>50	0.008585252961218894	0.32790356263672416
>100	6.603541314913818E-4	0.08066932486996395
>500	0.0	0.0
>1k	2.2011804381484926E-4	0.32596433477310577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	2380	0.3253032632837861	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.1004613018964635E-4	0.0
2	0.0	0.0	0.0	4.1004613018964635E-4	0.0
3	0.0	0.0	0.0	4.1004613018964635E-4	0.0
4	0.0	0.0	0.0	8.200922603792927E-4	0.0
5	0.0	0.0	0.0	0.0010934563471723903	0.0
6	0.0	0.0	0.0	0.001230138390568939	0.0
7	0.0	0.0	0.0	0.0013668204339654878	0.0
8	0.0	0.0	0.0	0.0013668204339654878	0.0
9	0.0	0.0	0.0	0.001776866564155134	0.0
10	0.0	0.0	0.0	0.0021869126943447805	0.0
11	0.0	0.0	0.0	0.0021869126943447805	0.0
12	0.0	0.0	0.0	0.0023235947377413294	0.0
13	0.0	0.0	0.0	0.002460276781137878	0.0
14	0.0	0.0	0.0	0.002460276781137878	0.0
15	0.0	0.0	0.0	0.0025969588245344268	0.0
16	0.0	0.0	0.0	0.0027336408679309756	0.0
17	0.0	0.0	0.0	0.003143686998120622	0.0
18	0.0	0.0	0.0	0.0032803690415171708	0.0
19	0.0	0.0	0.0	0.0034170510849137197	0.0
20	0.0	0.0	0.0	0.0034170510849137197	0.0
21	0.0	0.0	0.0	0.003553733128310268	0.0
22	0.0	0.0	0.0	0.003827097215103366	0.0
23	0.0	0.0	0.0	0.003827097215103366	0.0
24	0.0	0.0	0.0	0.004237143345293012	0.0
25	0.0	0.0	0.0	0.005330599692465402	0.0
26	0.0	0.0	0.0	0.005740645822655049	0.0
27	0.0	0.0	0.0	0.008610968733982573	0.0
28	0.0	0.0	0.0	0.017085255424568596	0.0
29	0.0	0.0	0.0	0.02665299846232701	0.0
30	0.0	0.0	0.0	0.03786092602084401	0.0
31	0.0	0.0	0.0	0.051939176490688535	0.0
32	0.0	0.0	0.0	0.06588074491713651	0.0
33	0.0	0.0	0.0	0.08392277464548095	0.0
34	0.0	0.0	0.0	0.10825217837006663	0.0
35	0.0	0.0	0.0	0.13968904835127285	0.0
36	0.0	0.0	0.0	0.19723218862121988	0.0
37	0.0	0.0	0.0	0.30302409021014864	0.0
38	0.0	0.0	0.0	0.4755168289765932	0.0
39	0.0	0.0	0.0	0.7134802665299846	0.0
40	0.0	0.0	0.0	0.9938151375363061	0.0
41	0.0	0.0	0.0	1.2660857679822313	0.0
42	0.0	0.0	0.0	1.5275585169998291	1.3668204339654878E-4
43	0.0	2.7336408679309756E-4	0.0	1.8221083205193918	1.3668204339654878E-4
44	0.0	2.7336408679309756E-4	0.0	2.1366137023748504	1.3668204339654878E-4
45	0.0	2.7336408679309756E-4	0.0	2.496360840594567	1.3668204339654878E-4
46	0.0	2.7336408679309756E-4	0.0	2.9069536989577993	1.3668204339654878E-4
47	0.0	2.7336408679309756E-4	0.0	3.345703058260721	1.3668204339654878E-4
48	0.0	2.7336408679309756E-4	0.0	3.8738424739449853	1.3668204339654878E-4
49	0.0	2.7336408679309756E-4	0.0	4.430001708525542	1.3668204339654878E-4
50	0.0	2.7336408679309756E-4	0.0	5.0133606697420126	1.3668204339654878E-4
51	0.0	2.7336408679309756E-4	0.0	5.544780454467794	1.3668204339654878E-4
52	0.0	2.7336408679309756E-4	0.0	6.065812403895438	1.3668204339654878E-4
53	0.0	2.7336408679309756E-4	0.0	6.510985819237997	1.3668204339654878E-4
54	0.0	2.7336408679309756E-4	0.0	6.941124209806937	1.3668204339654878E-4
55	0.0	2.7336408679309756E-4	0.0	7.395865368187255	1.3668204339654878E-4
56	0.0	2.7336408679309756E-4	0.0	7.863591320690245	1.3668204339654878E-4
57	0.0	2.7336408679309756E-4	0.0	8.33186400136682	1.3668204339654878E-4
58	0.0	2.7336408679309756E-4	0.0	8.84551512045105	1.3668204339654878E-4
59	0.0	2.7336408679309756E-4	0.0	9.383222279173074	1.3668204339654878E-4
60	0.0	2.7336408679309756E-4	0.0	9.942115154621561	1.3668204339654878E-4
61	0.0	2.7336408679309756E-4	0.0	10.477772082692637	1.3668204339654878E-4
62	0.0	2.7336408679309756E-4	0.0	10.95766273705792	1.3668204339654878E-4
63	0.0	2.7336408679309756E-4	0.0	11.411857167264651	1.3668204339654878E-4
64	0.0	2.7336408679309756E-4	0.0	11.845275926875107	1.3668204339654878E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	465	0.0	46.666668	47
TATGCCG	480	0.0	46.666664	43
CTCGTAT	460	0.0	46.41304	39
GCCGTCT	480	0.0	45.9375	46
TCTCGTA	435	0.0	45.057472	38
CGTCTTC	495	0.0	44.545452	48
ATCTCGT	455	0.0	43.076923	37
TCGTATG	520	0.0	43.076923	40
ATGCCGT	520	0.0	43.076923	44
CGTATGC	505	0.0	42.9703	41
TGCCGTC	515	0.0	42.815533	45
TATCTCG	470	0.0	40.957447	36
GCACGAT	35	0.0012554695	40.0	16
GTATGCC	645	0.0	35.271317	42
AGGACCG	40	0.0024188606	35.0	30
TTCGTAA	45	0.004307448	31.111113	20
GTAAGCG	45	0.004307448	31.111113	46
CGGTCAT	700	0.0	31.000002	28
CTGCTTG	750	0.0	29.400002	54
ACTACGC	60	4.982543E-4	29.166666	8
>>END_MODULE