FastQCFastQC Report
Wed 25 May 2016
SRR1781163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268442
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG200.006946261552.542
GGTCTAA200.006946261552.51
TACACGT200.006946261552.528
CGGTAAG350.001254050340.028
CCAAACG350.001254050340.09
GGACTAT459.183059E-538.888896
CTTCGTC400.002416141835.021
AGTGCGC552.9873886E-431.81818410
CAGTATT552.9873886E-431.81818414
TCGTTCG450.00430263631.11111323
TCTTCGT450.00430263631.11111320
TTTAACG450.00430263631.11111349
CCATACG450.00430263631.11111323
TTGCGAT450.00430263631.11111349
TTCGGTA450.00430263631.11111326
CCGTGCG952.8109935E-729.4736829
CGTGCGA604.974239E-429.16666810
TAACGGC500.007200680727.99999851
TCTAGCA1004.4033732E-727.9999983
CGATGTG500.007200680727.99999815