##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781163_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268442
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.685935881866474	34.0	33.0	34.0	24.0	34.0
2	31.730150274547203	34.0	34.0	34.0	24.0	34.0
3	31.689601478159155	34.0	34.0	34.0	23.0	34.0
4	31.807906363385758	34.0	34.0	34.0	23.0	34.0
5	31.840077186133318	34.0	34.0	34.0	27.0	34.0
6	34.710041647730236	38.0	35.0	38.0	27.0	38.0
7	34.65921130076516	38.0	35.0	38.0	26.0	38.0
8	34.500249588365456	37.0	34.0	38.0	26.0	38.0
9	34.73095119243636	38.0	35.0	38.0	27.0	38.0
10-11	34.604901617481616	38.0	35.0	38.0	26.0	38.0
12-13	34.54645696277036	38.0	34.0	38.0	26.0	38.0
14-15	34.41718881546107	37.5	34.0	38.0	25.5	38.0
16-17	34.39210518473264	37.0	34.0	38.0	25.0	38.0
18-19	34.459734318772774	37.5	34.0	38.0	25.5	38.0
20-21	34.39011220300847	37.0	34.0	38.0	25.0	38.0
22-23	34.46088168021397	37.5	34.0	38.0	25.5	38.0
24-25	34.41970332511306	37.0	34.0	38.0	25.5	38.0
26-27	34.35136454057115	37.0	34.0	38.0	24.5	38.0
28-29	34.41801208454713	37.0	34.0	38.0	25.5	38.0
30-31	34.48837924020832	37.0	34.0	38.0	26.0	38.0
32-33	34.43512751357835	37.0	34.0	38.0	25.5	38.0
34-35	34.47088756602916	37.0	34.0	38.0	25.5	38.0
36-37	34.33429753913322	37.0	34.0	38.0	24.5	38.0
38-39	34.3395780094024	37.0	34.0	38.0	25.0	38.0
40-41	34.338549854344706	37.0	34.0	38.0	24.5	38.0
42-43	34.42690041051698	37.0	34.0	38.0	25.0	38.0
44-45	34.46083511522042	37.0	34.0	38.0	25.5	38.0
46-47	34.44907279784832	37.0	34.0	38.0	25.5	38.0
48-49	34.416732478524224	37.0	34.0	38.0	25.0	38.0
50-51	34.289947176671305	37.0	34.0	38.0	24.5	38.0
52-53	34.374933877709154	37.0	34.0	38.0	25.0	38.0
54-55	34.34000268214363	37.0	34.0	38.0	25.0	38.0
56-57	34.32438105810566	37.0	34.0	38.0	24.5	38.0
58-59	34.31849896811974	37.0	34.0	38.0	24.5	38.0
60-61	34.29206308997847	37.0	34.0	38.0	24.0	38.0
62-63	34.27174398939063	37.0	34.0	38.0	24.0	38.0
64-65	34.27591248761371	37.0	34.0	38.0	24.0	38.0
66-67	34.17767338941	37.0	34.0	38.0	24.0	38.0
68-69	33.95710060273728	37.0	34.0	38.0	23.5	38.0
70-71	34.12714850880265	37.0	34.0	38.0	24.0	38.0
72-73	34.114324509577486	37.0	34.0	38.0	24.0	38.0
74-75	34.12934265129898	37.0	34.0	38.0	24.0	38.0
76	32.83809165480812	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	28.0
14	101.0
15	284.0
16	572.0
17	963.0
18	1228.0
19	1365.0
20	1501.0
21	1569.0
22	1840.0
23	2258.0
24	2693.0
25	3308.0
26	4165.0
27	4946.0
28	5952.0
29	7081.0
30	8664.0
31	10605.0
32	13026.0
33	16189.0
34	20768.0
35	28267.0
36	42714.0
37	88348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.042191609360685	18.368213617839235	9.685146139575775	24.90444863322431
2	23.529850023468757	20.680072417877977	31.15607840799875	24.633999150654518
3	19.638879161979126	22.097138301756058	29.36239485624455	28.901587680020263
4	14.14942520171955	16.507848995313697	36.60604525372334	32.73668054924342
5	13.76051437554481	33.309616229949114	36.158648795643	16.771220598863067
6	30.05341936060676	36.87314205675714	18.66399445690317	14.409444125732934
7	29.58255414577451	29.417155288665708	22.193993488351303	18.806297077208484
8	26.17250653772509	34.513973223266106	20.037102986864948	19.27641725214385
9	25.03334053538567	17.273377489364556	21.494028505226456	36.199253470023315
10-11	25.282742640868417	24.812249945984608	27.374814671325648	22.530192741821324
12-13	25.19315159326782	23.169250713375703	27.203455495041762	24.43414219831472
14-15	23.078728365903995	24.391861184166412	24.380499325738896	28.1489111241907
16-17	23.284545637418884	27.491599675162604	25.528233286892515	23.695621400525997
18-19	22.9090455293881	27.195818836098674	26.725512401189082	23.169623233324145
20-21	22.891909611759708	27.157076761460573	26.740413199126813	23.2106004276529
22-23	22.930092906475142	26.569054022842924	27.220218892721704	23.28063417796023
24-25	22.828208700575917	26.86204096229353	26.79778127118707	23.51196906594348
26-27	22.260115779199978	27.056496375380902	27.08611171128214	23.597276134136983
28-29	22.638223526869865	26.721042161807762	27.92800679476386	22.712727516558513
30-31	22.897683670960582	26.929094553013318	27.07922009223594	23.094001683790168
32-33	22.57079741620164	27.02743981940233	27.125226305868676	23.276536458527357
34-35	23.2217760261062	26.36770699070935	27.32620081805381	23.08431616513064
36-37	23.10908874170212	26.676712287943023	26.82721034711409	23.386988623240775
38-39	22.314503691672687	27.022597060072567	27.66351763136916	22.999381616885582
40-41	22.624626548751685	26.272341883907885	27.990031366179657	23.113000201160773
42-43	22.324375470306435	26.423026203053173	27.675810789667786	23.576787536972603
44-45	21.927641725214386	26.254460926382606	28.028214660895088	23.789682687507916
46-47	22.16400563250162	25.735168118252734	28.310212261866624	23.790613987379025
48-49	21.55325917702893	25.88324479775892	28.978885569322237	23.584610455889912
50-51	21.4599429299439	25.946014409071605	28.788155355719297	23.8058873052652
52-53	21.439826852727965	25.817122506910245	28.868992184531482	23.874058455830312
54-55	20.743214549139104	26.391920787358163	28.660939793325934	24.2039248701768
56-57	20.61711654659107	26.417997183749193	28.785547716080195	24.179338553579544
58-59	20.913456165577667	26.15220420053494	28.612139680079867	24.322199953807527
60-61	20.677837298187317	25.948808308684928	28.76971561827136	24.603638774856393
62-63	20.335677725542205	25.809672107941378	29.01166732478524	24.842982841731175
64-65	20.88626220934131	25.71970854039234	28.51211807392286	24.881911176343493
66-67	20.969334157844152	25.460062136327398	27.987982506463222	25.582621199365224
68-69	20.896879027871943	25.639244231528597	27.81867218989577	25.64520455070369
70-71	21.622920407387817	25.33936567303179	27.937506053449162	25.10020786613123
72-73	21.564248515508005	25.05494669239538	27.92484037520209	25.45596441689452
74-75	21.935837164080134	25.247912025688972	27.77043085657237	25.045819953658516
76	21.94515016279122	25.135038481310673	27.67376192995135	25.246049425946758
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	2.0
10	2.5
11	3.0
12	2.0
13	6.5
14	14.5
15	28.0
16	64.5
17	91.0
18	113.5
19	168.5
20	321.5
21	442.0
22	515.0
23	728.0
24	1039.0
25	1210.0
26	1422.0
27	1875.5
28	2347.5
29	2578.0
30	2981.5
31	3709.0
32	4718.0
33	5403.0
34	6000.0
35	7307.5
36	8786.0
37	9554.0
38	10274.0
39	11984.5
40	13836.5
41	15178.0
42	15658.0
43	15889.0
44	16218.5
45	16248.5
46	16180.0
47	15813.5
48	14532.5
49	13129.0
50	12640.0
51	11769.0
52	9939.5
53	8306.5
54	7632.0
55	7063.0
56	5993.0
57	5033.5
58	4575.0
59	4107.0
60	3448.5
61	3022.0
62	2786.0
63	2573.5
64	2278.5
65	2139.5
66	2055.5
67	2028.0
68	1924.5
69	1797.5
70	1760.0
71	1746.0
72	1701.0
73	1576.0
74	1413.5
75	1331.0
76	1216.0
77	993.0
78	813.0
79	741.0
80	664.5
81	502.5
82	356.0
83	295.0
84	239.5
85	174.0
86	137.5
87	111.0
88	77.0
89	37.5
90	24.5
91	15.5
92	14.0
93	10.0
94	5.0
95	2.5
96	1.0
97	2.0
98	2.0
99	5.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	268442.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.16957889345111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58772314535425	40.04634118763608
2	15.018392736781342	16.57116805889383
3	5.4889153600835225	9.084634469928107
4	2.066850666008341	4.5610912351731665
5	1.039418856460893	2.8672150302429986
6	0.6769119452191263	2.240696817941163
7	0.5119788174144448	1.9771959031385413
8	0.3929607741440731	1.7343584344938452
9	0.29996141454258535	1.489387043213852
>10	1.87856304139173	18.08590221396837
>50	0.03697856742073912	1.2618896289300514
>100	0.001344675178935968	0.08011997644000325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.450398968864782E-4	0.0
2	0.0	0.0	0.0	7.450398968864782E-4	0.0
3	0.0	0.0	0.0	7.450398968864782E-4	0.0
4	0.0	0.0	0.0	0.0014900797937729565	0.0
5	0.0	0.0	0.0	0.002235119690659435	0.0
6	0.0	0.0	0.0	0.002235119690659435	0.0
7	0.0	0.0	0.0	0.002607639639102674	0.0
8	0.0	0.0	0.0	0.002607639639102674	0.0
9	0.0	0.0	0.0	0.0033526795359891523	0.0
10	0.0	0.0	0.0	0.0033526795359891523	0.0
11	0.0	0.0	0.0	0.0037251994844323916	0.0
12	0.0	0.0	0.0	0.0037251994844323916	0.0
13	0.0	0.0	0.0	0.0037251994844323916	0.0
14	0.0	0.0	0.0	0.0037251994844323916	0.0
15	0.0	0.0	0.0	0.004097719432875631	0.0
16	0.0	0.0	0.0	0.004097719432875631	0.0
17	0.0	0.0	0.0	0.004097719432875631	0.0
18	0.0	0.0	0.0	0.0048427593297621085	0.0
19	0.0	0.0	0.0	0.0048427593297621085	0.0
20	0.0	0.0	0.0	0.005215279278205348	0.0
21	0.0	0.0	0.0	0.005215279278205348	0.0
22	0.0	0.0	0.0	0.006332839123535066	0.0
23	0.0	0.0	0.0	0.007077879020421543	0.0
24	0.0	0.0	0.0	0.0085679588141945	0.0
25	0.0	0.0	0.0	0.010058038607967456	0.0
26	0.0	0.0	0.0	0.011920638350183652	0.0
27	0.0	0.0	0.0	0.02235119690659435	0.0
28	0.0	0.0	0.0	0.03613443499899419	0.0
29	0.0	0.0	0.0	0.05699555211181559	0.0
30	0.0	0.0	0.0	0.06891619046199925	0.0
31	0.0	0.0	0.0	0.09163990731703683	0.0
32	0.0	0.0	0.0	0.11399110422363118	0.0
33	0.0	0.0	0.0	0.15012553922262536	0.0
34	0.0	0.0	0.0	0.20116077215934913	0.0
35	0.0	0.0	0.0	0.28386020071374823	0.0
36	0.0	0.0	0.0	0.39636122514360644	0.0
37	0.0	0.0	0.0	0.6016197167358313	0.0
38	0.0	0.0	0.0	0.8787745583776011	0.0
39	0.0	0.0	0.0	1.2162776316671757	0.0
40	0.0	0.0	0.0	1.596993018976166	0.0
41	0.0	0.0	0.0	2.0242733998405615	0.0
42	0.0	0.0	0.0	2.4821004164773024	0.0
43	0.0	0.0	0.0	3.0446055386265933	0.0
44	0.0	0.0	0.0	3.661871093197041	0.0
45	0.0	0.0	0.0	4.355130717249908	0.0
46	0.0	0.0	0.0	5.110228652744355	0.0
47	0.0	0.0	0.0	5.829937193136693	0.0
48	0.0	0.0	0.0	6.567154171105862	0.0
49	0.0	0.0	0.0	7.29021539103419	0.0
50	0.0	0.0	0.0	7.950320739675609	0.0
51	0.0	0.0	0.0	8.55194045641144	0.0
52	0.0	0.0	0.0	9.193047287682255	0.0
53	0.0	0.0	0.0	9.860975555240984	0.0
54	0.0	0.0	0.0	10.534864141974802	0.0
55	0.0	0.0	0.0	11.252337562676482	0.0
56	0.0	0.0	0.0	11.954537665491987	0.0
57	0.0	0.0	0.0	12.644817129957309	0.0
58	0.0	0.0	0.0	13.327273675505323	3.725199484432391E-4
59	0.0	0.0	0.0	13.95608734847751	3.725199484432391E-4
60	0.0	0.0	0.0	14.624388135984683	3.725199484432391E-4
61	0.0	0.0	0.0	15.242398730452015	3.725199484432391E-4
62	0.0	0.0	0.0	15.881270442032172	3.725199484432391E-4
63	0.0	0.0	0.0	16.515299394282565	3.725199484432391E-4
64	0.0	0.0	0.0	17.091960274472697	3.725199484432391E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	20	0.0069462615	52.5	42
GGTCTAA	20	0.0069462615	52.5	1
TACACGT	20	0.0069462615	52.5	28
CGGTAAG	35	0.0012540503	40.0	28
CCAAACG	35	0.0012540503	40.0	9
GGACTAT	45	9.183059E-5	38.88889	6
CTTCGTC	40	0.0024161418	35.0	21
AGTGCGC	55	2.9873886E-4	31.818184	10
CAGTATT	55	2.9873886E-4	31.818184	14
TCGTTCG	45	0.004302636	31.111113	23
TCTTCGT	45	0.004302636	31.111113	20
TTTAACG	45	0.004302636	31.111113	49
CCATACG	45	0.004302636	31.111113	23
TTGCGAT	45	0.004302636	31.111113	49
TTCGGTA	45	0.004302636	31.111113	26
CCGTGCG	95	2.8109935E-7	29.473682	9
CGTGCGA	60	4.974239E-4	29.166668	10
TAACGGC	50	0.0072006807	27.999998	51
TCTAGCA	100	4.4033732E-7	27.999998	3
CGATGTG	50	0.0072006807	27.999998	15
>>END_MODULE