FastQCFastQC Report
Wed 25 May 2016
SRR1781163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250791
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT24570.9797002284770986No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2960.11802656395165695No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT207.916552E-570.000013
TCTCGTA3400.056.61764538
CTCGTAT3900.056.53846439
CGTAGTG252.3874213E-455.99999654
ATCTCGT3400.055.58823437
CCGTCTT4000.055.12499647
TATGCCG4200.054.1666743
TCGTATG4250.053.5294140
CGTATGC4300.052.9069841
GCCGTCT4250.052.70588346
CGGAAAT200.00694573152.50000415
ATCGGTT200.00694573152.50000422
GACGAAA200.00694573152.50000457
TATCGGT200.00694573152.50000421
GCGTAAG200.00694573152.5000041
CGTCTTC4250.051.8823548
ATGCCGT4350.051.49425544
CATCTCG3700.051.0810836
GTATGCC4600.049.4565242
TCTTCTG4350.048.27586450
Produced by FastQC (version 0.11.5)