##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781163_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250791
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1819562902975	34.0	34.0	34.0	32.0	34.0
2	33.317040882647305	34.0	34.0	34.0	33.0	34.0
3	33.35739320789023	34.0	34.0	34.0	33.0	34.0
4	33.422040663341186	34.0	34.0	34.0	34.0	34.0
5	33.42842845237668	34.0	34.0	34.0	34.0	34.0
6	36.839467923490076	38.0	37.0	38.0	34.0	38.0
7	36.7774441666567	38.0	37.0	38.0	34.0	38.0
8	36.55276305768548	38.0	37.0	38.0	34.0	38.0
9	36.5397801356508	38.0	37.0	38.0	34.0	38.0
10-11	36.454228820013476	38.0	37.0	38.0	34.0	38.0
12-13	36.40869090198612	38.0	37.0	38.0	34.0	38.0
14-15	36.40643005530501	38.0	37.0	38.0	34.0	38.0
16-17	36.366023900379204	38.0	37.0	38.0	34.0	38.0
18-19	36.359526458285984	38.0	37.0	38.0	34.0	38.0
20-21	36.30878899163048	38.0	37.0	38.0	34.0	38.0
22-23	36.36349988635956	38.0	37.0	38.0	34.0	38.0
24-25	36.35562879050684	38.0	37.0	38.0	34.0	38.0
26-27	36.15420609192515	38.0	37.0	38.0	33.5	38.0
28-29	36.123513204221844	38.0	37.0	38.0	33.5	38.0
30-31	36.165059750948004	38.0	37.0	38.0	34.0	38.0
32-33	36.08355164260281	38.0	37.0	38.0	33.0	38.0
34-35	36.099672635780394	38.0	37.0	38.0	33.5	38.0
36-37	36.07605934822223	38.0	37.0	38.0	32.5	38.0
38-39	36.03212635222157	38.0	37.0	38.0	32.0	38.0
40-41	36.06460359422786	38.0	37.0	38.0	32.5	38.0
42-43	36.06149343477238	38.0	37.0	38.0	32.5	38.0
44-45	36.032868005630185	38.0	37.0	38.0	32.0	38.0
46-47	36.06124821066147	38.0	37.0	38.0	32.5	38.0
48-49	36.037044790283545	38.0	37.0	38.0	32.0	38.0
50-51	35.95329178479292	38.0	37.0	38.0	32.0	38.0
52-53	35.98106989485269	38.0	37.0	38.0	32.0	38.0
54-55	35.996507051688454	38.0	37.0	38.0	32.0	38.0
56-57	35.948166002767245	38.0	37.0	38.0	32.0	38.0
58-59	35.950085928123414	38.0	37.0	38.0	32.0	38.0
60-61	35.96177295038498	38.0	37.0	38.0	32.0	38.0
62-63	35.920902265232804	38.0	37.0	38.0	32.0	38.0
64-65	35.88567173463163	38.0	37.0	38.0	32.0	38.0
66-67	35.8623794314788	38.0	36.5	38.0	31.5	38.0
68-69	35.786399830934926	38.0	36.5	38.0	31.0	38.0
70-71	35.702124478151134	38.0	36.0	38.0	31.0	38.0
72-73	35.63069647634883	38.0	36.0	38.0	31.0	38.0
74-75	35.62761422858077	38.0	36.0	38.0	31.0	38.0
76	34.75377106833977	37.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	1.0
21	5.0
22	35.0
23	71.0
24	160.0
25	301.0
26	576.0
27	1002.0
28	1780.0
29	3003.0
30	4594.0
31	6925.0
32	10034.0
33	14385.0
34	20195.0
35	28273.0
36	44547.0
37	114902.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.209720444513565	18.375858782811186	10.551415321921441	26.863005450753814
2	23.112870876546605	20.86598003915611	31.66580937912445	24.35533970517283
3	19.956457767623238	22.56460558791982	29.55967319401414	27.9192634504428
4	14.029211574578035	17.83118213970996	36.386473198799	31.753133086913003
5	13.54514316701955	34.2344820986399	36.33782711500812	15.882547619332431
6	30.089197778229682	37.8853308133067	17.997456049060773	14.028015359402849
7	28.767778748041195	29.725149626581498	21.688178602900425	19.818893022476882
8	25.691113317463543	35.846980154790245	19.124290744085712	19.337615783660496
9	24.456220518280162	17.103484574805318	20.928183228265766	37.51211167864876
10-11	24.900215717469926	25.58604575124307	27.23682269299935	22.276915838287657
12-13	24.63565279455802	23.272764971629766	27.168638428013764	24.922943805798454
14-15	22.598298982020886	24.322045049463497	23.84216339501816	29.237492573497452
16-17	23.573413718993105	28.09191717406127	24.91995326786049	23.414715839085133
18-19	23.194811616046827	26.990003628519364	26.237783652523415	23.577401102910393
20-21	22.363242700096894	27.057988524309085	26.0938390931094	24.48492968248462
22-23	22.677049814387278	27.349067550270945	26.441738339892577	23.532144295449196
24-25	23.2781479399181	27.29723155934623	26.399472070369352	23.025148430366322
26-27	22.555035866518335	27.646925128892185	26.686563712413925	23.111475292175555
28-29	22.507386628706772	26.666826162023362	27.742223604515313	23.083563604754556
30-31	22.350283702365715	27.373988699753976	26.79402370898477	23.481703888895535
32-33	22.098081669597395	27.768739707565267	26.4846027170034	23.64857590583394
34-35	22.62122643954528	26.268885247078245	27.51853136675558	23.59135694662089
36-37	22.54965289823	26.961493833510776	26.64230375093205	23.846549517327176
38-39	21.946561080740537	27.051209971649698	27.502781200282307	23.499447747327455
40-41	22.089708163371093	26.06114254498766	28.238254163825655	23.610895127815592
42-43	22.737059942342427	25.9682364997149	27.84250631003505	23.45219724790762
44-45	21.704925615353023	26.823729719168547	28.039483075548965	23.43186158992946
46-47	22.308814909625944	25.745740477130358	27.866031875147034	24.07941273809666
48-49	21.907085979959408	25.907030156584565	28.069787193320334	24.11609667013569
50-51	21.17719535390026	25.81053546578625	28.79888034259603	24.21338883771746
52-53	20.929379443440954	25.73597138653301	29.71557990518001	23.619069264846026
54-55	20.272258573872268	26.071908481564332	29.159937956306248	24.495894988257156
56-57	20.684753440115475	25.95747056313823	28.586352779804695	24.771423216941596
58-59	20.274451635026775	25.79837394483853	29.349936799964908	24.577237620169782
60-61	20.791415959902867	26.448516892551964	28.234864887495963	24.525202260049202
62-63	19.71701536339023	27.058387262700816	27.991833837737403	25.232763536171554
64-65	19.87511513571061	27.453935747295553	27.314975417778147	25.355973699215685
66-67	19.95326786048941	27.40070417199979	26.80100163084002	25.845026336670774
68-69	20.096813681511698	27.582130140236295	26.493773700013158	25.82728247823885
70-71	20.310736828674074	27.413862538926836	26.338664465630746	25.93673616676834
72-73	20.017664110753575	26.696133433815405	26.48958694690001	26.79661550853101
74-75	20.068503255698968	26.921221255946186	26.141089592529237	26.86918589582561
76	20.223213751689652	26.765314544780317	26.046389224493705	26.96508247903633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.0
12	8.0
13	6.0
14	12.0
15	25.0
16	67.0
17	104.0
18	117.0
19	199.0
20	326.0
21	384.0
22	482.0
23	720.0
24	1029.5
25	1199.0
26	1394.5
27	1770.5
28	2310.0
29	2669.0
30	2956.5
31	3666.0
32	4677.5
33	5267.0
34	5684.0
35	6652.0
36	8084.0
37	8965.0
38	9450.0
39	10926.5
40	12826.5
41	14320.5
42	14906.0
43	15070.5
44	15448.0
45	15469.0
46	15277.0
47	14981.0
48	13721.0
49	12047.0
50	11337.0
51	10723.5
52	9127.0
53	7589.5
54	7035.0
55	6565.0
56	5516.5
57	4500.0
58	4062.0
59	3693.0
60	3018.5
61	2591.5
62	2470.0
63	2321.5
64	2057.5
65	1941.0
66	1884.5
67	1829.0
68	1828.0
69	1748.5
70	1677.0
71	1684.0
72	1603.0
73	1413.0
74	1308.5
75	1313.0
76	1206.5
77	1021.0
78	843.5
79	745.0
80	659.0
81	514.0
82	383.5
83	312.0
84	252.0
85	153.0
86	93.0
87	72.0
88	54.0
89	28.5
90	18.5
91	11.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	250791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.01658703592441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.96284341266886	30.712774377896185
2	19.858610136800152	19.070853640923108
3	7.220113960524788	10.400556911844944
4	2.831378928469901	5.438126110022297
5	1.3490978768937818	3.2389537812925555
6	0.8371172862756447	2.4117309021438813
7	0.6221339400039205	2.091092393474058
8	0.47446133600645296	1.8225611228427863
9	0.3898506573413958	1.684736821732093
>10	2.3955003256442757	20.204828530429268
>50	0.053915338860604414	1.611447453798611
>100	0.004147333758508032	0.3337600015529501
>500	0.0	0.0
>1k	8.294667517016065E-4	0.9785779520472535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	2457	0.9797002284770986	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	296	0.11802656395165695	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.987383917285708E-4	0.0
2	0.0	0.0	0.0	3.987383917285708E-4	0.0
3	0.0	0.0	0.0	3.987383917285708E-4	0.0
4	0.0	0.0	0.0	0.0015949535669142832	0.0
5	0.0	0.0	0.0	0.0023924303503714247	0.0
6	0.0	0.0	0.0	0.0023924303503714247	0.0
7	0.0	0.0	0.0	0.0027911687420999957	0.0
8	0.0	0.0	0.0	0.0031899071338285664	0.0
9	0.0	0.0	0.0	0.004386122309014279	0.0
10	0.0	0.0	0.0	0.004386122309014279	0.0
11	0.0	0.0	0.0	0.004386122309014279	0.0
12	0.0	0.0	0.0	0.004386122309014279	0.0
13	0.0	0.0	0.0	0.004386122309014279	0.0
14	0.0	0.0	0.0	0.004386122309014279	0.0
15	0.0	0.0	0.0	0.004784860700742849	0.0
16	0.0	0.0	0.0	0.004784860700742849	0.0
17	0.0	0.0	0.0	0.004784860700742849	0.0
18	0.0	0.0	0.0	0.0055823374841999914	0.0
19	0.0	0.0	0.0	0.0055823374841999914	0.0
20	0.0	0.0	0.0	0.005981075875928562	0.0
21	0.0	0.0	0.0	0.005981075875928562	0.0
22	0.0	0.0	0.0	0.006379814267657133	0.0
23	0.0	0.0	0.0	0.0075760294428428455	0.0
24	0.0	0.0	0.0	0.008373506226299987	0.0
25	0.0	0.0	0.0	0.00996845979321427	3.987383917285708E-4
26	0.0	0.0	0.0	0.011563413360128554	3.987383917285708E-4
27	0.0	0.0	0.0	0.023126826720257107	3.987383917285708E-4
28	0.0	0.0	0.0	0.03628519364729994	3.987383917285708E-4
29	0.0	0.0	0.0	0.05701959001718562	3.987383917285708E-4
30	0.0	0.0	0.0	0.07017795694422846	3.987383917285708E-4
31	0.0	0.0	0.0	0.09450099883967128	3.987383917285708E-4
32	0.0	0.0	0.0	0.11962151751857124	3.987383917285708E-4
33	0.0	0.0	0.0	0.15750166473278546	3.987383917285708E-4
34	0.0	0.0	0.0	0.21372377796651396	3.987383917285708E-4
35	0.0	0.0	0.0	0.2990537937964281	3.987383917285708E-4
36	0.0	0.0	0.0	0.4138904506142565	3.987383917285708E-4
37	0.0	0.0	0.0	0.6367852115905276	3.987383917285708E-4
38	0.0	0.0	0.0	0.9166995625839843	3.987383917285708E-4
39	0.0	0.0	0.0	1.2667918705216694	3.987383917285708E-4
40	0.0	0.0	0.0	1.658352971199126	3.987383917285708E-4
41	0.0	0.0	0.0	2.1053387083268538	3.987383917285708E-4
42	0.0	0.0	0.0	2.5830273016176815	3.987383917285708E-4
43	0.0	0.0	0.0	3.1731601213759664	3.987383917285708E-4
44	0.0	0.0	0.0	3.8087491177913084	3.987383917285708E-4
45	0.0	0.0	0.0	4.526478222902735	3.987383917285708E-4
46	0.0	0.0	0.0	5.30920168586592	3.987383917285708E-4
47	0.0	0.0	0.0	6.040487896296119	3.987383917285708E-4
48	0.0	0.0	0.0	6.78812238078719	3.987383917285708E-4
49	0.0	0.0	0.0	7.525788405485045	3.987383917285708E-4
50	0.0	0.0	0.0	8.197263857155958	3.987383917285708E-4
51	0.0	0.0	0.0	8.808928550067586	3.987383917285708E-4
52	0.0	0.0	0.0	9.460467082152071	3.987383917285708E-4
53	0.0	0.0	0.0	10.132341272214713	3.987383917285708E-4
54	0.0	0.0	0.0	10.816975090812669	3.987383917285708E-4
55	0.0	0.0	0.0	11.548261301242867	3.987383917285708E-4
56	0.0	0.0	0.0	12.257218161736267	3.987383917285708E-4
57	0.0	0.0	0.0	12.988105633774737	3.987383917285708E-4
58	0.0	0.0	0.0	13.672340713980963	3.987383917285708E-4
59	0.0	0.0	0.0	14.349398503136078	3.987383917285708E-4
60	0.0	0.0	0.0	15.028449984249834	3.987383917285708E-4
61	0.0	0.0	0.0	15.652874305696775	3.987383917285708E-4
62	0.0	0.0	0.0	16.29922923868879	3.987383917285708E-4
63	0.0	0.0	0.0	16.95156524755673	3.987383917285708E-4
64	0.0	0.0	0.0	17.535716991439088	3.987383917285708E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAT	20	7.916552E-5	70.00001	3
TCTCGTA	340	0.0	56.617645	38
CTCGTAT	390	0.0	56.538464	39
CGTAGTG	25	2.3874213E-4	55.999996	54
ATCTCGT	340	0.0	55.588234	37
CCGTCTT	400	0.0	55.124996	47
TATGCCG	420	0.0	54.16667	43
TCGTATG	425	0.0	53.52941	40
CGTATGC	430	0.0	52.90698	41
GCCGTCT	425	0.0	52.705883	46
CGGAAAT	20	0.006945731	52.500004	15
ATCGGTT	20	0.006945731	52.500004	22
GACGAAA	20	0.006945731	52.500004	57
TATCGGT	20	0.006945731	52.500004	21
GCGTAAG	20	0.006945731	52.500004	1
CGTCTTC	425	0.0	51.88235	48
ATGCCGT	435	0.0	51.494255	44
CATCTCG	370	0.0	51.08108	36
GTATGCC	460	0.0	49.45652	42
TCTTCTG	435	0.0	48.275864	50
>>END_MODULE