FastQCFastQC Report
Wed 25 May 2016
SRR1781162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172829
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG200.006942095752.55
TTCGTAA200.006942095752.523
GGTTCGG200.006942095752.523
CAACCCG305.86547E-446.66666436
CGCAAGG305.86547E-446.66666445
TTTCCGA404.5785382E-543.7526
GGTCGAT350.001252810540.010
GCACGCA350.001252810540.037
CCCGTAC350.001252810540.012
CGTCATC350.001252810540.041
TAGAGCC400.002413768635.04
GAAGTAC400.002413768635.01
ACGCTTA400.002413768635.059
TGCAGAC400.002413768635.065
CGTACTA652.0937556E-532.30769314
GTTATAC652.0937556E-532.3076931
TTCCGAC552.983015E-431.8181827
GTACTAA552.983015E-431.8181815
GTCGATG450.004298436531.11110911
GGTCGGT450.004298436531.11110911
Produced by FastQC (version 0.11.5)