##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781162_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172829
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96567127044651	34.0	32.0	34.0	21.0	34.0
2	31.141804905426753	34.0	32.0	34.0	23.0	34.0
3	31.165302119435975	34.0	32.0	34.0	23.0	34.0
4	31.254245525924468	34.0	33.0	34.0	23.0	34.0
5	31.274317388864137	34.0	33.0	34.0	23.0	34.0
6	34.12154210230922	37.0	34.0	38.0	24.0	38.0
7	34.043644295806835	37.0	34.0	38.0	24.0	38.0
8	33.84004420554421	37.0	34.0	38.0	23.0	38.0
9	34.15892008864253	37.0	34.0	38.0	24.0	38.0
10-11	33.99124278911525	37.0	34.0	38.0	23.0	38.0
12-13	33.97124903806653	37.0	34.0	38.0	23.0	38.0
14-15	33.79042001053064	37.0	34.0	38.0	23.0	38.0
16-17	33.767889069542726	37.0	34.0	38.0	22.5	38.0
18-19	33.81170984036244	37.0	34.0	38.0	23.0	38.0
20-21	33.73181873412448	37.0	34.0	38.0	22.5	38.0
22-23	33.82225494563991	37.0	34.0	38.0	23.0	38.0
24-25	33.82476031221612	37.0	34.0	38.0	23.0	38.0
26-27	33.71503914273646	37.0	34.0	38.0	22.5	38.0
28-29	33.82699951975653	37.0	34.0	38.0	23.0	38.0
30-31	33.897809395413965	37.0	34.0	38.0	23.0	38.0
32-33	33.86411713312002	37.0	34.0	38.0	23.0	38.0
34-35	33.86742097680366	37.0	34.0	38.0	23.0	38.0
36-37	33.71468040664472	37.0	34.0	38.0	22.5	38.0
38-39	33.722251474000316	37.0	34.0	38.0	22.5	38.0
40-41	33.72580990458778	37.0	34.0	38.0	22.5	38.0
42-43	33.81440904014951	37.0	34.0	38.0	23.0	38.0
44-45	33.82672757465472	37.0	34.0	38.0	23.0	38.0
46-47	33.81381596838494	37.0	34.0	38.0	23.0	38.0
48-49	33.777812172725646	37.0	34.0	38.0	23.0	38.0
50-51	33.642953439526934	37.0	34.0	38.0	22.5	38.0
52-53	33.701109188851404	37.0	34.0	38.0	23.0	38.0
54-55	33.69284668660931	37.0	34.0	38.0	23.0	38.0
56-57	33.663366680360355	37.0	34.0	38.0	22.5	38.0
58-59	33.64037864015877	37.0	34.0	38.0	22.5	38.0
60-61	33.62984800004629	37.0	34.0	38.0	22.5	38.0
62-63	33.5809528493482	37.0	34.0	38.0	22.0	38.0
64-65	33.5990169473873	37.0	34.0	38.0	22.0	38.0
66-67	33.50321126662771	37.0	34.0	38.0	22.0	38.0
68-69	33.279333908082556	37.0	33.5	38.0	21.5	38.0
70-71	33.46782658002997	37.0	34.0	38.0	22.0	38.0
72-73	33.431715742149755	37.0	33.5	38.0	22.0	38.0
74-75	33.4446794230135	37.0	33.0	38.0	22.0	38.0
76	32.056020690972	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	26.0
14	80.0
15	268.0
16	610.0
17	1071.0
18	1537.0
19	1617.0
20	1667.0
21	1590.0
22	1700.0
23	1915.0
24	2277.0
25	2657.0
26	2984.0
27	3569.0
28	4395.0
29	5123.0
30	6079.0
31	7152.0
32	8608.0
33	10443.0
34	12923.0
35	16985.0
36	24986.0
37	52559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.793819324303215	18.282811333746075	9.528493481996655	26.39487585995406
2	23.099711275306806	19.41051559634089	32.31633580012613	25.173437328226168
3	19.754786523095085	21.76602306325906	29.258399921309504	29.220790492336356
4	13.932846918051947	15.651887125424551	36.738626040768615	33.67663991575488
5	14.121472669517269	33.87683779921194	35.94246335973708	16.059226171533712
6	30.959503324094918	37.06727458933396	17.380185038390547	14.593037048180571
7	29.63044396484386	28.749804720272635	22.011352261483893	19.6083990533996
8	26.339908232993302	33.882623865207805	19.298265916021037	20.479201985777852
9	24.921743457405874	14.818115015419867	21.27189302721187	38.98824849996239
10-11	25.6701710939715	24.131656145669997	27.504643317961687	22.69352944239682
12-13	25.628222115501448	22.50229996123336	26.387932580759017	25.48154534250618
14-15	23.779863333121178	23.698279802579428	23.779284726521592	28.742572137777806
16-17	24.02663904784498	26.453315126512333	25.132934866255084	24.387110959387602
18-19	23.288337026772126	25.98956193694345	26.605777965503474	24.116323070780947
20-21	23.363266581418625	26.20625010848874	26.239519987964982	24.19096332212765
22-23	23.83020210728524	25.733817819926053	26.59767747310926	23.83830259967945
24-25	23.45844736705067	26.04742259690214	26.286965729131108	24.207164306916084
26-27	22.78350276863258	26.394007950054675	26.41859873053712	24.40389055077562
28-29	23.325367849145685	25.787338930387836	27.370985193457116	23.516308027009355
30-31	23.720845459963318	25.835652581453346	26.65090928027125	23.79259267831209
32-33	22.99990163687807	26.388800490658397	26.44376811761915	24.167529754844384
34-35	24.001758964062745	25.515393828582013	26.80076838956425	23.682078817790998
36-37	23.71361287746848	25.59842387562273	26.24848839025858	24.439474856650214
38-39	22.93017954162785	26.04308304740524	27.120159232536206	23.906578178430703
40-41	23.354008875825237	25.11557666826748	27.6009813167929	23.929433139114384
42-43	22.997008603880136	25.53419854306858	27.047254800988256	24.42153805206302
44-45	22.52775865161518	25.31027778902846	27.431160279814154	24.730803279542208
46-47	22.786395801630512	25.07883514919371	27.619207424679885	24.515561624495888
48-49	22.162079280676274	25.033125227826346	28.343622887362653	24.461172604134724
50-51	22.19274543045438	24.995805102152996	27.98054724612189	24.830902221270737
52-53	21.849631716899363	25.00650932424535	28.392804448327535	24.751054510527748
54-55	21.09715383413663	25.42831353534418	28.71884926719474	24.755683363324444
56-57	21.259452985320753	25.43554611783902	28.147475250102705	25.15752564673753
58-59	21.34595467195899	25.301887993334454	28.208518246359116	25.14363908834744
60-61	20.997922802307485	24.980182723964152	28.24728488853144	25.774609585196927
62-63	20.80524680464505	24.971503624970346	28.561178968807315	25.66207060157728
64-65	21.51259337264001	25.026760555230894	28.06213077666364	25.39851529546546
66-67	21.64654080044437	24.88934148782901	27.327589698488104	26.136528013238518
68-69	21.41914840680673	24.937365835594722	27.13751743052381	26.505968327074736
70-71	22.024434627390736	24.442727906566336	27.537124953349707	25.99571251269322
72-73	22.246266540916164	23.980929126477616	27.273779284726523	26.499025047879698
74-75	22.284743879788692	24.351237350213218	27.334822280982934	26.029196489015156
76	22.755440348552618	23.84148493597718	27.204925099375682	26.198149616094526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	3.5
15	6.5
16	20.0
17	33.0
18	46.0
19	71.0
20	102.0
21	121.0
22	164.0
23	254.0
24	381.0
25	461.0
26	561.5
27	786.5
28	1040.0
29	1169.0
30	1383.5
31	1834.5
32	2394.5
33	2718.0
34	3042.5
35	3870.0
36	4885.0
37	5397.0
38	6045.5
39	7252.5
40	8458.0
41	9592.0
42	10079.0
43	10216.5
44	10530.0
45	10799.5
46	10893.0
47	10670.0
48	9884.5
49	8866.0
50	8410.0
51	7883.5
52	6709.5
53	5674.5
54	5287.0
55	4886.5
56	4106.5
57	3483.5
58	3240.0
59	2847.5
60	2321.0
61	2108.5
62	2030.0
63	1955.5
64	1798.5
65	1677.0
66	1562.0
67	1486.0
68	1491.5
69	1484.5
70	1446.0
71	1420.0
72	1402.0
73	1315.0
74	1162.5
75	1079.0
76	1027.0
77	861.0
78	684.5
79	622.0
80	530.0
81	397.0
82	305.5
83	255.0
84	222.5
85	144.0
86	72.0
87	46.0
88	37.0
89	27.5
90	19.0
91	6.5
92	2.0
93	4.0
94	3.5
95	2.5
96	4.0
97	2.5
98	0.5
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.8930329979343745E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	172829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.76701826661034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75625504439064	39.65653912248523
2	13.24078557976863	14.238351202633817
3	5.077212806026365	8.189597810552627
4	2.2814097390368575	4.906583964496699
5	1.171912832929782	3.150512934750534
6	0.8189400053806833	2.6419177337136706
7	0.5918751681463547	2.2276354084094683
8	0.44874899112187244	1.9302316162218145
9	0.387409200968523	1.8746853826614747
>10	2.198547215496368	20.29751951350757
>50	0.026903416733925208	0.8864253105670923
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.786065995868749E-4	0.0
2	0.0	0.0	0.0	5.786065995868749E-4	0.0
3	0.0	0.0	0.0	5.786065995868749E-4	0.0
4	0.0	0.0	0.0	0.0011572131991737498	0.0
5	0.0	0.0	0.0	0.0011572131991737498	0.0
6	0.0	0.0	0.0	0.0011572131991737498	0.0
7	0.0	0.0	0.0	0.0011572131991737498	0.0
8	0.0	0.0	0.0	0.0011572131991737498	0.0
9	0.0	0.0	0.0	0.0023144263983474996	0.0
10	0.0	0.0	0.0	0.0023144263983474996	0.0
11	0.0	0.0	0.0	0.0023144263983474996	0.0
12	0.0	0.0	0.0	0.0023144263983474996	0.0
13	0.0	0.0	0.0	0.0023144263983474996	0.0
14	0.0	0.0	0.0	0.0034716395975212494	0.0
15	0.0	0.0	0.0	0.0034716395975212494	0.0
16	0.0	0.0	0.0	0.0034716395975212494	0.0
17	0.0	0.0	0.0	0.0034716395975212494	0.0
18	0.0	0.0	0.0	0.0034716395975212494	0.0
19	0.0	0.0	0.0	0.005207459396281874	0.0
20	0.0	0.0	0.0	0.0057860659958687485	0.0
21	0.0	0.0	0.0	0.006943279195042499	0.0
22	0.0	0.0	0.0	0.008679098993803123	0.0
23	0.0	0.0	0.0	0.008679098993803123	0.0
24	0.0	0.0	0.0	0.010414918792563748	0.0
25	0.0	0.0	0.0	0.010993525392150623	0.0
26	0.0	0.0	0.0	0.012150738591324373	0.0
27	0.0	0.0	0.0	0.01677959138801937	0.0
28	0.0	0.0	0.0	0.03298057617645187	0.0
29	0.0	0.0	0.0	0.054967626960753115	0.0
30	0.0	0.0	0.0	0.07116861174918561	0.0
31	0.0	0.0	0.0	0.08910541633637874	0.0
32	0.0	0.0	0.0	0.1105138605210931	0.0
33	0.0	0.0	0.0	0.14349443669754497	0.0
34	0.0	0.0	0.0	0.20656255605251433	0.0
35	0.0	0.0	0.0	0.27946698760046057	0.0
36	0.0	0.0	0.0	0.4177539649017237	0.0
37	0.0	0.0	0.0	0.6173732417591955	0.0
38	0.0	0.0	0.0	0.8713815389778335	0.0
39	0.0	0.0	0.0	1.1757286103605298	0.0
40	0.0	0.0	0.0	1.5223139635130678	0.0
41	0.0	0.0	0.0	1.900722679642884	0.0
42	0.0	0.0	0.0	2.321369677542542	0.0
43	0.0	0.0	0.0	2.8276504521810577	0.0
44	0.0	0.0	0.0	3.437501808145624	0.0
45	0.0	0.0	0.0	4.157867024631283	0.0
46	0.0	0.0	0.0	4.932042654878521	0.0
47	0.0	0.0	0.0	5.774493863877011	0.0
48	0.0	0.0	0.0	6.571813758107725	0.0
49	0.0	0.0	0.0	7.3292097969669445	0.0
50	0.0	0.0	0.0	8.033374028664172	0.0
51	0.0	0.0	0.0	8.658269156217996	0.0
52	0.0	0.0	0.0	9.31845928634662	0.0
53	0.0	0.0	0.0	9.956083759091356	0.0
54	0.0	0.0	0.0	10.682813648172472	0.0
55	0.0	0.0	0.0	11.457567885019296	0.0
56	0.0	0.0	0.0	12.24389425385786	0.0
57	0.0	0.0	0.0	13.031377835895595	0.0
58	0.0	0.0	0.0	13.755793298578364	0.0
59	0.0	0.0	0.0	14.478472941462371	0.0
60	0.0	0.0	0.0	15.224875454929439	0.0
61	0.0	0.0	0.0	15.869443206869217	0.0
62	0.0	0.0	0.0	16.454414479051547	0.0
63	0.0	0.0	0.0	17.05442952282314	0.0
64	0.0	0.0	0.0	17.68684653617159	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCG	20	0.0069420957	52.5	5
TTCGTAA	20	0.0069420957	52.5	23
GGTTCGG	20	0.0069420957	52.5	23
CAACCCG	30	5.86547E-4	46.666664	36
CGCAAGG	30	5.86547E-4	46.666664	45
TTTCCGA	40	4.5785382E-5	43.75	26
GGTCGAT	35	0.0012528105	40.0	10
GCACGCA	35	0.0012528105	40.0	37
CCCGTAC	35	0.0012528105	40.0	12
CGTCATC	35	0.0012528105	40.0	41
TAGAGCC	40	0.0024137686	35.0	4
GAAGTAC	40	0.0024137686	35.0	1
ACGCTTA	40	0.0024137686	35.0	59
TGCAGAC	40	0.0024137686	35.0	65
CGTACTA	65	2.0937556E-5	32.307693	14
GTTATAC	65	2.0937556E-5	32.307693	1
TTCCGAC	55	2.983015E-4	31.81818	27
GTACTAA	55	2.983015E-4	31.81818	15
GTCGATG	45	0.0042984365	31.111109	11
GGTCGGT	45	0.0042984365	31.111109	11
>>END_MODULE