FastQCFastQC Report
Wed 25 May 2016
SRR1781162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155483
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT31392.0188702301859367No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3140.20195133873156548No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGACCTCGTATGCCGT2650.1704366393753658No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC2300.14792613983522312No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGAACTCGTATGCCGT1710.1099798691818398No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC150.002221276870.0000137
TAACCGG150.002221276870.0000122
GTGAATA150.002221276870.000012
AAAGCGT150.002221276870.0000135
GTAGCGT252.3845879E-456.0000043
CGTGGTA200.00694078952.5000047
CGGGTCT200.00694078952.5000044
GGTAACA200.00694078952.50000440
GACACTC200.00694078952.5000047
TAGCGTG200.00694078952.5000044
GGCCATT200.00694078952.50000444
GAAAGCG200.00694078952.50000434
TCGGATT200.00694078952.50000427
CTCGGAT200.00694078952.50000426
CGGGCTA200.00694078952.50000441
CGCAGAA407.442177E-752.50000432
AATTCAC200.00694078952.50000458
CGTATGC6150.052.35772341
TATGCCG6150.052.35772343
TCTCGTA4900.052.14285738
Produced by FastQC (version 0.11.5)