FastQCFastQC Report
Wed 25 May 2016
SRR1781161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242783
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACGA200.006945465752.527
CGCAATA305.870192E-446.66666857
TATTCCC503.469513E-642.00000429
ACATTCG350.001253813540.08
CCGTATA350.001253813540.021
CGAGCAA459.1804664E-538.88888537
CATTCGA501.7065191E-435.0000049
ACCCGTA400.002415688235.019
CGATAGA400.002415688235.037
GATCCGC450.004301834431.11110934
TCTAGAC450.004301834431.11110935
TACATTC450.004301834431.11110945
ATTCCCG450.004301834431.11110929
TAACCGG450.004301834431.11110922
AATCTAG450.004301834431.11110933
CGGCTTT450.004301834431.11110926
GCGCTAT450.004301834431.11110919
GCTGCGT604.9728557E-429.1666686
GGCTTAA604.9728557E-429.16666811
GAAATCG500.007199344728.00000248