##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 242783 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.589612946540736 34.0 33.0 34.0 24.0 34.0 2 31.652034944786084 34.0 33.0 34.0 24.0 34.0 3 31.651680718995976 34.0 34.0 34.0 23.0 34.0 4 31.78048298274591 34.0 34.0 34.0 23.0 34.0 5 31.751967806642146 34.0 34.0 34.0 23.0 34.0 6 34.6503956207807 38.0 35.0 38.0 26.0 38.0 7 34.632700806893396 38.0 35.0 38.0 26.0 38.0 8 34.40523430388454 37.0 34.0 38.0 25.0 38.0 9 34.6794503733787 38.0 35.0 38.0 26.0 38.0 10-11 34.567164916818726 38.0 34.5 38.0 26.0 38.0 12-13 34.53110802650927 38.0 34.5 38.0 26.0 38.0 14-15 34.393050584266604 38.0 34.0 38.0 25.0 38.0 16-17 34.36586787378029 37.5 34.0 38.0 25.0 38.0 18-19 34.426901801196955 38.0 34.0 38.0 25.0 38.0 20-21 34.37210389524802 37.5 34.0 38.0 25.0 38.0 22-23 34.41951042700683 38.0 34.0 38.0 25.0 38.0 24-25 34.419844058274265 37.5 34.0 38.0 25.5 38.0 26-27 34.34928928302229 37.0 34.0 38.0 24.5 38.0 28-29 34.42251104896142 37.5 34.0 38.0 25.5 38.0 30-31 34.49558247488498 38.0 34.0 38.0 26.0 38.0 32-33 34.43781071986095 37.5 34.0 38.0 25.5 38.0 34-35 34.45732403010095 38.0 34.0 38.0 25.5 38.0 36-37 34.323338124992276 37.0 34.0 38.0 24.5 38.0 38-39 34.35242788827883 37.0 34.0 38.0 25.0 38.0 40-41 34.350133658452194 37.0 34.0 38.0 24.5 38.0 42-43 34.42856377917729 37.0 34.0 38.0 25.0 38.0 44-45 34.44775993376801 37.0 34.0 38.0 25.0 38.0 46-47 34.43289686674932 37.5 34.0 38.0 25.0 38.0 48-49 34.400981534950965 37.0 34.0 38.0 25.0 38.0 50-51 34.2740780861922 37.0 34.0 38.0 24.0 38.0 52-53 34.37009592928665 37.0 34.0 38.0 25.0 38.0 54-55 34.33154298282828 37.0 34.0 38.0 24.5 38.0 56-57 34.31421887034924 37.0 34.0 38.0 24.5 38.0 58-59 34.32435755386497 37.0 34.0 38.0 24.5 38.0 60-61 34.27717138349884 37.0 34.0 38.0 24.0 38.0 62-63 34.245929492592154 37.0 34.0 38.0 24.0 38.0 64-65 34.2595507098932 37.0 34.0 38.0 24.0 38.0 66-67 34.16367496900524 37.0 34.0 38.0 24.0 38.0 68-69 33.959684162400166 37.0 34.0 38.0 23.5 38.0 70-71 34.12402639394027 37.0 34.0 38.0 24.0 38.0 72-73 34.101839914656296 37.0 34.0 38.0 24.0 38.0 74-75 34.11651145261406 37.0 34.0 38.0 24.0 38.0 76 32.766342783473306 37.0 31.0 38.0 21.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 19.0 14 89.0 15 240.0 16 501.0 17 967.0 18 1356.0 19 1490.0 20 1554.0 21 1596.0 22 1761.0 23 2070.0 24 2554.0 25 3000.0 26 3743.0 27 4460.0 28 5161.0 29 6218.0 30 7843.0 31 9400.0 32 11494.0 33 13966.0 34 18364.0 35 24327.0 36 37091.0 37 83513.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75148589481142 19.44040562971872 10.329800686209497 25.47830778926037 2 22.207485697103998 19.841586931539688 33.201253794540804 24.74967357681551 3 19.351437291737888 22.611138341646654 28.70341004106548 29.33401432554998 4 13.66158256550088 16.012241384281438 37.68509327259322 32.64108277762446 5 13.429276349662045 34.50406329932491 36.41770634682 15.648954004193044 6 30.54291280690987 37.098561266645525 18.024738140644114 14.333787785800489 7 29.441929624397094 28.960429684121213 22.492925781459164 19.10471491002253 8 25.551212399550217 34.62268774996602 19.977098890779008 19.849000959704757 9 24.637227482978627 15.93439408854821 21.861497716067436 37.566880712405734 10-11 25.223347598472714 24.785713991506817 27.762034409328496 22.228904000691976 12-13 24.968799298138666 23.06462973107672 27.274150167021578 24.69242080376303 14-15 22.66303653880214 24.570295284266198 24.50212741419292 28.264540762738743 16-17 23.23906533818266 27.41728209965278 25.73223825391399 23.611414308250577 18-19 22.747268136566397 27.15305437365878 26.777410279961945 23.32226720981288 20-21 22.65727007245153 27.24634756140257 26.67896846154797 23.417413904597932 22-23 23.06009893608696 26.522038198720665 27.3270780903111 23.090784774881275 24-25 22.712463393235936 26.66681769316633 27.058113624100532 23.562605289497203 26-27 22.06600132628726 27.03175263506918 27.327284035537907 23.574962003105654 28-29 22.91284809891961 26.63263078551628 27.88560154541298 22.56891957015112 30-31 22.786397729659818 26.6495182941145 27.198980159236854 23.36510381698883 32-33 22.301190775301404 27.263029124773976 26.96564421726398 23.47013588266065 34-35 23.148655383614177 26.200351754447386 27.47350514657122 23.17748771536722 36-37 23.176252044006375 26.37066845701717 26.779881622683632 23.67319787629282 38-39 22.37141809764275 26.875028317468686 27.741851777101363 23.0117018077872 40-41 22.706079091204902 25.95692449636095 28.149211435726556 23.187784976707597 42-43 22.324462585930647 26.12827092506477 27.779333808380326 23.767932680624263 44-45 21.60056511370236 25.98781628038207 28.175366479531107 24.236252126384468 46-47 21.84708155019091 25.633796435500017 28.717826206942004 23.801295807367072 48-49 21.308534782089357 25.54276864525111 29.367377452292786 23.78131912036675 50-51 21.164167178097312 25.824295770296935 28.83089013645931 24.180646915146447 52-53 21.01897579319804 25.59343117104575 29.299621472673127 24.087971563083084 54-55 20.27242434602093 26.113442868734634 29.471997627510987 24.14213515773345 56-57 20.1846104418994 26.314402044636843 29.11856957799503 24.382417935468723 58-59 20.507492318211398 26.076686080516676 28.668929327544873 24.74689227372705 60-61 20.288323911319804 25.98354494248968 28.627681154943215 25.10044999124731 62-63 20.028255801500936 25.576854956298245 29.14837179033042 25.246517451870403 64-65 20.755571848111277 25.665306055201558 28.313143836265308 25.265978260421857 66-67 20.66129148517706 25.58071524924572 27.996457734803787 25.76153553077343 68-69 20.7276486724716 25.19914985460207 27.874801262037547 26.198400210888778 70-71 21.39281043732559 25.247701131671352 27.966595615417088 25.392892815585967 72-73 21.602006730290015 24.736287137073024 27.753590655029388 25.908115477607573 74-75 21.87653171762438 24.70312995555702 27.854297870938243 25.566040455880355 76 22.119753030484013 24.67265006198951 27.737938817792017 25.469658089734455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 6.0 14 15.5 15 34.5 16 64.5 17 81.0 18 92.5 19 158.5 20 244.0 21 275.0 22 380.5 23 597.5 24 846.0 25 983.0 26 1173.0 27 1496.5 28 1892.0 29 2154.0 30 2454.5 31 3028.0 32 4017.5 33 4734.0 34 5094.5 35 6145.5 36 7657.5 37 8479.0 38 9319.0 39 11047.5 40 12740.0 41 14072.0 42 14600.0 43 14770.5 44 15181.5 45 15405.0 46 15388.0 47 15320.5 48 14170.5 49 12600.0 50 12112.0 51 11137.0 52 9166.5 53 7459.0 54 6747.0 55 6294.5 56 5212.0 57 4087.5 58 3593.0 59 3244.5 60 2687.5 61 2379.5 62 2280.0 63 2137.0 64 1899.0 65 1792.0 66 1679.5 67 1579.0 68 1577.0 69 1561.5 70 1536.5 71 1525.0 72 1493.5 73 1416.5 74 1265.0 75 1159.0 76 1086.5 77 900.5 78 749.0 79 711.0 80 621.5 81 463.0 82 354.0 83 314.0 84 241.0 85 129.5 86 76.5 87 62.0 88 46.0 89 21.0 90 12.5 91 10.0 92 7.0 93 4.5 94 2.0 95 2.0 96 2.0 97 2.5 98 2.0 99 4.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.178356804224348E-4 58-59 4.1189045361495657E-4 60-61 2.0594522680747828E-4 62-63 4.1189045361495657E-4 64-65 0.0 66-67 2.0594522680747828E-4 68-69 4.1189045361495657E-4 70-71 2.0594522680747828E-4 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 242783.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.25418972180486 #Duplication Level Percentage of deduplicated Percentage of total 1 65.64661396890814 29.70784323143054 2 17.34665175220406 15.700173388646425 3 6.726090308186588 9.131513006780063 4 2.894697411244888 5.239887433427742 5 1.5026754375038553 3.40011796695478 6 0.9484844220895291 2.5753736391249595 7 0.7295965774282935 2.3112111354723637 8 0.5662392558038658 2.049975896806539 9 0.5167397115721818 2.1046173249850413 >10 3.035098103271474 25.16576729889034 >50 0.08414328865802898 2.463894108012787 >100 0.002969763129106905 0.1496255694684129 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0024713427216897393 0.0 2 0.0 0.0 0.0 0.0024713427216897393 0.0 3 0.0 0.0 0.0 0.0024713427216897393 0.0 4 0.0 0.0 0.0 0.0032951236289196525 0.0 5 0.0 0.0 0.0 0.003707014082534609 0.0 6 0.0 0.0 0.0 0.003707014082534609 0.0 7 0.0 0.0 0.0 0.003707014082534609 0.0 8 0.0 0.0 0.0 0.003707014082534609 0.0 9 0.0 0.0 0.0 0.004530794989764522 0.0 10 0.0 0.0 0.0 0.004530794989764522 0.0 11 0.0 0.0 0.0 0.004942685443379479 0.0 12 0.0 0.0 0.0 0.004942685443379479 0.0 13 0.0 0.0 0.0 0.004942685443379479 0.0 14 0.0 0.0 0.0 0.004942685443379479 0.0 15 0.0 0.0 0.0 0.004942685443379479 0.0 16 0.0 0.0 0.0 0.005354575896994436 0.0 17 0.0 0.0 0.0 0.005354575896994436 0.0 18 0.0 0.0 0.0 0.005354575896994436 0.0 19 0.0 0.0 0.0 0.005354575896994436 0.0 20 0.0 0.0 0.0 0.005766466350609392 0.0 21 0.0 0.0 0.0 0.006590247257839305 0.0 22 0.0 0.0 0.0 0.006590247257839305 0.0 23 0.0 0.0 0.0 0.007002137711454261 0.0 24 0.0 0.0 0.0 0.009885370886758957 0.0 25 0.0 0.0 0.0 0.009885370886758957 0.0 26 0.0 0.0 0.0 0.010709151793988871 0.0 27 0.0 0.0 0.0 0.02388964630966748 0.0 28 0.0 0.0 0.0 0.04036526445426574 0.0 29 0.0 0.0 0.0 0.06137167758862853 0.0 30 0.0 0.0 0.0 0.08690888571275583 0.0 31 0.0 0.0 0.0 0.10462017521819897 0.0 32 0.0 0.0 0.0 0.13221683561040107 0.0 33 0.0 0.0 0.0 0.16969886688936212 0.0 34 0.0 0.0 0.0 0.2323062158388355 0.0 35 0.0 0.0 0.0 0.3138605256545969 0.0 36 0.0 0.0 0.0 0.464612431677671 0.0 37 0.0 0.0 0.0 0.7080396897641104 0.0 38 0.0 0.0 0.0 1.0363163812952307 0.0 39 0.0 0.0 0.0 1.4259647504149797 0.0 40 0.0 0.0 0.0 1.8716302212263627 0.0 41 0.0 0.0 0.0 2.343656681069103 0.0 42 0.0 0.0 0.0 2.899296902995679 0.0 43 0.0 0.0 0.0 3.5669713283055238 0.0 44 0.0 0.0 0.0 4.31290493980221 0.0 45 0.0 0.0 0.0 5.123505352516445 0.0 46 0.0 0.0 0.0 6.011129280056676 0.0 47 0.0 0.0 0.0 6.921819072999345 0.0 48 0.0 0.0 0.0 7.788436587405213 0.0 49 0.0 0.0 0.0 8.638166593212869 0.0 50 0.0 0.0 0.0 9.414991988730677 0.0 51 0.0 0.0 0.0 10.151864010247834 0.0 52 0.0 0.0 0.0 10.926629953497567 0.0 53 0.0 0.0 0.0 11.665149536829185 0.0 54 0.0 0.0 0.0 12.463393235934971 0.0 55 0.0 0.0 0.0 13.297471404505258 0.0 56 0.0 0.0 0.0 14.100657789054424 0.0 57 0.0 0.0 0.0 14.923614915377106 0.0 58 0.0 0.0 0.0 15.714444586317823 0.0 59 0.0 0.0 0.0 16.478089487319952 0.0 60 0.0 0.0 0.0 17.231849017435323 0.0 61 0.0 0.0 0.0 17.945655173550044 0.0 62 0.0 0.0 0.0 18.619919846117728 0.0 63 0.0 0.0 0.0 19.31725038408785 0.0 64 0.0 0.0 0.0 20.03723489700679 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAACGA 20 0.0069454657 52.5 27 CGCAATA 30 5.870192E-4 46.666668 57 TATTCCC 50 3.469513E-6 42.000004 29 ACATTCG 35 0.0012538135 40.0 8 CCGTATA 35 0.0012538135 40.0 21 CGAGCAA 45 9.1804664E-5 38.888885 37 CATTCGA 50 1.7065191E-4 35.000004 9 ACCCGTA 40 0.0024156882 35.0 19 CGATAGA 40 0.0024156882 35.0 37 GATCCGC 45 0.0043018344 31.111109 34 TCTAGAC 45 0.0043018344 31.111109 35 TACATTC 45 0.0043018344 31.111109 45 ATTCCCG 45 0.0043018344 31.111109 29 TAACCGG 45 0.0043018344 31.111109 22 AATCTAG 45 0.0043018344 31.111109 33 CGGCTTT 45 0.0043018344 31.111109 26 GCGCTAT 45 0.0043018344 31.111109 19 GCTGCGT 60 4.9728557E-4 29.166668 6 GGCTTAA 60 4.9728557E-4 29.166668 11 GAAATCG 50 0.0071993447 28.000002 48 >>END_MODULE