FastQCFastQC Report
Wed 25 May 2016
SRR1781161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218588
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT28541.3056526433290023No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG3580.16377843248485735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC2250.1029333723717679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.063.0864243
CGTATGC4250.061.76470641
CTCGTAT4200.061.66666439
TCGTATG4150.061.5662740
CCGTCTT4300.060.2325647
GCCGTCT4300.059.41860646
TCTCGTA4100.058.9024438
CGTCTTC4200.058.33333248
ATCTCGT4150.058.19277237
ATGCCGT4550.056.9230844
GTATGCC4500.056.7777842
TGCCGTC4700.055.10638445
CTTGAAA4650.054.19355457
CTGCTTG4600.052.554
ACCGATG200.006944543752.526
TACGGTC200.006944543752.510
GCGTTGG200.006944543752.550
TATCTCG4600.052.536
GGCGCTA407.4562377E-752.518
GCGCTAT407.4562377E-752.519
Produced by FastQC (version 0.11.5)