FastQCFastQC Report
Wed 25 May 2016
SRR1781160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174742
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT207.9098405E-570.0000153
AACGCTA207.9098405E-570.0000151
CATCGTA150.002221738770.0000114
GCTAATC207.9098405E-570.0000154
TGACTCG252.3854095E-455.99999643
CGAATCA200.006942221452.50000441
CGTCAAG200.006942221452.50000419
CTCGTAA305.865647E-446.66666830
CTATCAG305.865647E-446.66666819
ACGCTAA305.865647E-446.66666852
CGTAAGA305.865647E-446.66666832
TTCTAAA350.001252848440.046
CCTAGAT350.001252848440.042
TAAACGC350.001252848440.049
TCGTAAG350.001252848440.031
GCGTCAA350.001252848440.026
GACTCGC350.001252848440.044
TCAGTGG459.169897E-538.88888558
GTTTGCA400.002413840535.00000430
TCCATAG400.002413840535.00000452
Produced by FastQC (version 0.11.5)