##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781160_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174742
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251582332810656	34.0	32.0	34.0	23.0	34.0
2	31.21681107003468	34.0	32.0	34.0	23.0	34.0
3	31.124663790044753	34.0	32.0	34.0	23.0	34.0
4	31.30369916791613	34.0	33.0	34.0	23.0	34.0
5	31.34589280195946	34.0	33.0	34.0	23.0	34.0
6	34.012143617447435	37.0	34.0	38.0	23.0	38.0
7	33.95313662428037	37.0	34.0	38.0	24.0	38.0
8	33.70082178297147	37.0	34.0	38.0	22.0	38.0
9	34.09337194263543	37.0	34.0	38.0	24.0	38.0
10-11	33.94321628457955	37.0	34.0	38.0	23.0	38.0
12-13	33.896252761213674	37.0	34.0	38.0	23.0	38.0
14-15	33.77521717732428	37.0	34.0	38.0	22.5	38.0
16-17	33.73908962928203	37.0	34.0	38.0	22.5	38.0
18-19	33.78203293999153	37.0	34.0	38.0	22.5	38.0
20-21	33.709912327889114	37.0	34.0	38.0	22.5	38.0
22-23	33.763966304609085	37.0	34.0	38.0	22.5	38.0
24-25	33.77391811928443	37.0	34.0	38.0	22.5	38.0
26-27	33.67467180185645	37.0	34.0	38.0	22.0	38.0
28-29	33.75444941685456	37.0	34.0	38.0	22.5	38.0
30-31	33.84524041157822	37.0	34.0	38.0	23.0	38.0
32-33	33.78475695596937	37.0	34.0	38.0	22.5	38.0
34-35	33.80559052774949	37.0	34.0	38.0	23.0	38.0
36-37	33.65577823305216	37.0	34.0	38.0	22.0	38.0
38-39	33.66852559773838	37.0	34.0	38.0	22.0	38.0
40-41	33.671552918016275	37.0	34.0	38.0	22.5	38.0
42-43	33.73486339861053	37.0	34.0	38.0	22.5	38.0
44-45	33.757222075974866	37.0	34.0	38.0	22.5	38.0
46-47	33.72718636618558	37.0	34.0	38.0	22.5	38.0
48-49	33.68414863055247	37.0	34.0	38.0	22.0	38.0
50-51	33.546623021368646	37.0	34.0	38.0	22.0	38.0
52-53	33.64786370763755	37.0	34.0	38.0	22.0	38.0
54-55	33.59660528092846	37.0	34.0	38.0	22.0	38.0
56-57	33.58352313696764	37.0	34.0	38.0	22.0	38.0
58-59	33.57279017065159	37.0	34.0	38.0	22.0	38.0
60-61	33.53859118013986	37.0	34.0	38.0	22.0	38.0
62-63	33.49828318320724	37.0	34.0	38.0	22.0	38.0
64-65	33.50931945382335	37.0	34.0	38.0	22.0	38.0
66-67	33.40970402078493	37.0	34.0	38.0	21.0	38.0
68-69	33.178354373876914	37.0	33.5	38.0	21.0	38.0
70-71	33.35216776733699	37.0	34.0	38.0	21.5	38.0
72-73	33.33183207242678	37.0	33.5	38.0	21.0	38.0
74-75	33.3390884847375	37.0	33.0	38.0	21.5	38.0
76	31.93788556843804	36.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	23.0
14	104.0
15	296.0
16	686.0
17	1214.0
18	1673.0
19	1806.0
20	1763.0
21	1763.0
22	1699.0
23	2079.0
24	2321.0
25	2644.0
26	3093.0
27	3666.0
28	4345.0
29	5081.0
30	5948.0
31	7241.0
32	8549.0
33	10257.0
34	12814.0
35	16979.0
36	25328.0
37	53365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.79904087168511	17.444575431207152	9.02416133499674	25.732222362110996
2	24.462350207734833	19.20774627736892	30.629156127319135	25.700747387577117
3	20.513671584392988	20.743152762358218	28.144922228199288	30.598253425049503
4	14.793810303189844	15.624749630884391	35.61536436575065	33.96607570017511
5	14.913987478682857	32.366574721589544	35.21935195888796	17.50008584083964
6	31.23404791063396	35.42193634043333	17.83772647674858	15.506289272184134
7	30.393379954447123	27.87194835815087	21.426445845875634	20.308225841526365
8	26.674182509070516	32.56515319728514	19.166542674342747	21.5941216193016
9	24.95736571631319	15.264790376669604	20.473040253631066	39.30480365338613
10-11	26.877339162880133	23.10806789438143	26.557725103295144	23.456867839443294
12-13	26.281031463529086	21.57952867656316	25.656682423229675	26.48275743667807
14-15	24.628023028235912	22.651394627507983	22.93180803699168	29.788774307264426
16-17	25.26181456089549	25.30587952524293	24.1149809433336	25.317324970527977
18-19	24.4663561135846	25.070675624635175	25.250369115610443	25.212599146169783
20-21	24.442606814618124	24.989699099243456	25.29529248835426	25.27240159778416
22-23	24.724737040894578	24.748200203728928	25.668986276911106	24.858076478465392
24-25	24.624017122386146	24.795412665529753	25.0414897391583	25.5390804729258
26-27	23.86547023611954	25.020887937645213	25.65181810898353	25.46182371725172
28-29	24.548477183504826	24.66379004475169	26.28017305513271	24.507559716610775
30-31	24.459488846413567	24.74562497853979	25.737658948621394	25.057227226425244
32-33	24.08922869144224	25.152224422291148	25.598882924540177	25.15966396172643
34-35	24.99399114122535	24.453193851506793	25.73508372343226	24.8177312838356
36-37	25.0228908905701	24.35047098007348	25.214602099094662	25.41203603026176
38-39	24.222854265145184	24.805141294022043	26.121653637934784	24.850350802897985
40-41	24.35647983884813	24.10525231484131	26.682480456902173	24.855787389408384
42-43	24.03686577926314	24.24603129184741	26.238111043710155	25.47899188517929
44-45	23.383330853486857	24.441462270089616	26.649288665575533	25.52591821084799
46-47	23.63427224136155	24.153037048906388	26.732268143892142	25.480422565839923
48-49	23.153277403257373	24.148172734660243	27.19180277208685	25.506747089995535
50-51	23.013642970779777	24.28151217223106	26.95001774044019	25.754827116548967
52-53	22.936958487369953	23.909249064334848	27.40754941571002	25.74624303258518
54-55	22.294010598482334	24.52301106774559	27.501115930915294	25.68186240285678
56-57	22.212747936958486	24.579666021906583	27.328289707111054	25.879296334023877
58-59	22.637946229298052	24.433450458390084	26.98750157374873	25.941101738563138
60-61	22.274330940274634	24.210333549843625	27.108328588228897	26.407006921652844
62-63	21.86601351138682	23.98113785219882	27.385023019717696	26.767825616696662
64-65	22.505465200123613	24.101818681255793	26.887926199768803	26.504789918851795
66-67	22.38249070770252	24.038079105421435	26.572107942303347	27.007322244572695
68-69	22.29887491272848	24.275503313456408	26.392052282794058	27.033569491021048
70-71	22.93673798153272	23.80344680571015	26.81131843322851	26.448496779528618
72-73	22.984457085302903	23.58419841823946	26.50822355243731	26.92312094402033
74-75	23.13382014627279	23.704375593732475	26.660447974728456	26.50135628526628
76	23.500932803790732	23.28289707111055	26.3422645958041	26.873905529294618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	1.0
12	1.0
13	2.0
14	5.0
15	13.0
16	19.5
17	20.0
18	35.0
19	64.5
20	111.0
21	143.0
22	155.5
23	253.0
24	411.0
25	484.0
26	586.5
27	778.5
28	1020.5
29	1173.0
30	1390.0
31	1713.5
32	2295.0
33	2770.0
34	3005.5
35	3632.5
36	4506.0
37	4988.0
38	5526.0
39	6772.0
40	8025.5
41	8977.5
42	9384.0
43	9467.5
44	9510.5
45	9656.5
46	9843.0
47	9736.5
48	9138.0
49	8229.0
50	7812.0
51	7173.5
52	6022.0
53	5116.5
54	4724.0
55	4421.5
56	3846.0
57	3344.0
58	3115.0
59	2910.0
60	2600.5
61	2453.0
62	2410.0
63	2368.5
64	2375.5
65	2441.5
66	2445.0
67	2431.0
68	2435.5
69	2424.5
70	2426.0
71	2443.0
72	2353.5
73	2273.5
74	2196.0
75	2109.0
76	1954.0
77	1590.5
78	1262.0
79	1142.0
80	1018.0
81	783.0
82	563.5
83	455.0
84	360.5
85	225.5
86	150.0
87	115.0
88	83.0
89	39.0
90	18.5
91	6.5
92	3.0
93	5.5
94	5.0
95	1.5
96	1.0
97	0.5
98	1.5
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.861361321262204E-4
62-63	2.861361321262204E-4
64-65	0.0
66-67	2.861361321262204E-4
68-69	0.0
70-71	2.861361321262204E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	174742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.13451831843518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35607684448807	43.054331528768124
2	12.953985456439431	14.802394387153633
3	4.740679901440333	8.125693880120407
4	1.9621787295418576	4.484325462682126
5	1.079749193693784	3.0845475043206556
6	0.7522185941224785	2.578658822721498
7	0.5338648610749415	2.1351478179258563
8	0.3996474288347122	1.826693067493791
9	0.3555760331737415	1.8284098842865482
>10	1.8499969951321142	17.548728983301096
>50	0.016025962058534828	0.531068661226265
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.722722642524408E-4	0.0
5	0.0	0.0	0.0	0.0017168167927573222	0.0
6	0.0	0.0	0.0	0.0017168167927573222	0.0
7	0.0	0.0	0.0	0.0017168167927573222	0.0
8	0.0	0.0	0.0	0.0017168167927573222	0.0
9	0.0	0.0	0.0	0.0034336335855146443	0.0
10	0.0	0.0	0.0	0.0034336335855146443	0.0
11	0.0	0.0	0.0	0.004005905849767085	0.0
12	0.0	0.0	0.0	0.004005905849767085	0.0
13	0.0	0.0	0.0	0.004005905849767085	0.0
14	0.0	0.0	0.0	0.004005905849767085	0.0
15	0.0	0.0	0.0	0.004005905849767085	0.0
16	0.0	0.0	0.0	0.004005905849767085	0.0
17	0.0	0.0	0.0	0.004578178114019526	0.0
18	0.0	0.0	0.0	0.004578178114019526	0.0
19	0.0	0.0	0.0	0.006867267171029289	0.0
20	0.0	0.0	0.0	0.006867267171029289	0.0
21	0.0	0.0	0.0	0.007439539435281729	0.0
22	0.0	0.0	0.0	0.010300900756543933	0.0
23	0.0	0.0	0.0	0.011445445285048815	0.0
24	0.0	0.0	0.0	0.012589989813553696	0.0
25	0.0	0.0	0.0	0.013162262077806137	0.0
26	0.0	0.0	0.0	0.014879078870563459	0.0
27	0.0	0.0	0.0	0.03147497453388424	0.0
28	0.0	0.0	0.0	0.048070870197205025	0.0
29	0.0	0.0	0.0	0.06752812718178801	0.0
30	0.0	0.0	0.0	0.08469629510936123	0.0
31	0.0	0.0	0.0	0.10300900756543933	0.0
32	0.0	0.0	0.0	0.12876125945679917	0.0
33	0.0	0.0	0.0	0.17339849606848953	0.0
34	0.0	0.0	0.0	0.2529443407995788	0.0
35	0.0	0.0	0.0	0.3542365315722608	0.0
36	0.0	0.0	0.0	0.5539595517963627	0.0
37	0.0	0.0	0.0	0.792024813725378	0.0
38	0.0	0.0	0.0	1.1147863707637546	0.0
39	0.0	0.0	0.0	1.4381202000663835	0.0
40	0.0	0.0	0.0	1.8118139886232274	0.0
41	0.0	0.0	0.0	2.1895136830298383	0.0
42	0.0	0.0	0.0	2.5740806446074784	0.0
43	0.0	0.0	0.0	3.0622288860148106	0.0
44	0.0	0.0	0.0	3.550949399686395	0.0
45	0.0	0.0	0.0	4.1506907326229525	0.0
46	0.0	0.0	0.0	4.828261093497843	0.0
47	0.0	0.0	0.0	5.532155978528345	0.0
48	0.0	0.0	0.0	6.208009522610477	0.0
49	0.0	0.0	0.0	6.8535326366872305	0.0
50	0.0	0.0	0.0	7.419509906032895	0.0
51	0.0	0.0	0.0	8.024973961611977	0.0
52	0.0	0.0	0.0	8.594384864543155	0.0
53	0.0	0.0	0.0	9.136326698790217	0.0
54	0.0	0.0	0.0	9.720616680591958	0.0
55	0.0	0.0	0.0	10.33065891428506	0.0
56	0.0	0.0	0.0	11.013951997802474	0.0
57	0.0	0.0	0.0	11.650318755651188	0.0
58	0.0	0.0	0.0	12.327316844261826	0.0
59	0.0	0.0	0.0	12.965400418903297	0.0
60	0.0	0.0	0.0	13.584026736560185	0.0
61	0.0	0.0	0.0	14.120818120428975	0.0
62	0.0	0.0	0.0	14.698813107323941	0.0
63	0.0	0.0	0.0	15.248194481006283	0.0
64	0.0	0.0	0.0	15.79814812695288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAT	20	7.9098405E-5	70.00001	53
AACGCTA	20	7.9098405E-5	70.00001	51
CATCGTA	15	0.0022217387	70.00001	14
GCTAATC	20	7.9098405E-5	70.00001	54
TGACTCG	25	2.3854095E-4	55.999996	43
CGAATCA	20	0.0069422214	52.500004	41
CGTCAAG	20	0.0069422214	52.500004	19
CTCGTAA	30	5.865647E-4	46.666668	30
CTATCAG	30	5.865647E-4	46.666668	19
ACGCTAA	30	5.865647E-4	46.666668	52
CGTAAGA	30	5.865647E-4	46.666668	32
TTCTAAA	35	0.0012528484	40.0	46
CCTAGAT	35	0.0012528484	40.0	42
TAAACGC	35	0.0012528484	40.0	49
TCGTAAG	35	0.0012528484	40.0	31
GCGTCAA	35	0.0012528484	40.0	26
GACTCGC	35	0.0012528484	40.0	44
TCAGTGG	45	9.169897E-5	38.888885	58
GTTTGCA	40	0.0024138405	35.000004	30
TCCATAG	40	0.0024138405	35.000004	52
>>END_MODULE