FastQCFastQC Report
Wed 25 May 2016
SRR1781160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157458
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT38342.4349350302937927No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG3850.2445096470169823No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT2480.1575023180784717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC2290.14543560822568558No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT1610.10224948875255625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACA150.0022213370.0000144
GATCTTA150.0022213370.000011
TATGCCG5350.057.5700943
CGTATGC5450.056.51376341
TCGTATG5450.056.51376340
CTCGTAT5300.056.13207639
CTTGAAA5050.055.44554557
GTATGCC5600.055.042
CCGTCTT5050.054.75247647
TCTCGTA5000.054.638
ATGCCGT5350.054.29906544
CGTCTTC5050.054.05940648
ATCTCGT5000.053.89999837
GCCGTCT5400.053.14814846
TGCCGTC5500.052.81818445
CATCTCG5050.052.67326736
CTCGAAA200.00694095652.49999657
GGGCAGT200.00694095652.49999637
ATGCAAG200.00694095652.49999638
GCTTGAA5350.052.33644556