##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781160_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157458
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27270764267297	34.0	34.0	34.0	32.0	34.0
2	33.30469712558269	34.0	34.0	34.0	33.0	34.0
3	33.35964511171233	34.0	34.0	34.0	33.0	34.0
4	33.40878837531278	34.0	34.0	34.0	34.0	34.0
5	33.41061743449047	34.0	34.0	34.0	34.0	34.0
6	36.810076337817065	38.0	37.0	38.0	34.0	38.0
7	36.71056408693112	38.0	37.0	38.0	34.0	38.0
8	36.52307917031843	38.0	37.0	38.0	34.0	38.0
9	36.48388141599665	38.0	37.0	38.0	34.0	38.0
10-11	36.3988111115345	38.0	37.0	38.0	34.0	38.0
12-13	36.3370168552884	38.0	37.0	38.0	34.0	38.0
14-15	36.34799121035451	38.0	37.0	38.0	34.0	38.0
16-17	36.27698179832082	38.0	37.0	38.0	34.0	38.0
18-19	36.271996341881646	38.0	37.0	38.0	34.0	38.0
20-21	36.21492715517789	38.0	37.0	38.0	33.5	38.0
22-23	36.277407308615636	38.0	37.0	38.0	34.0	38.0
24-25	36.25213707782393	38.0	37.0	38.0	34.0	38.0
26-27	36.03650179730468	38.0	37.0	38.0	32.5	38.0
28-29	36.033253311994315	38.0	37.0	38.0	32.0	38.0
30-31	36.03761002934115	38.0	37.0	38.0	32.0	38.0
32-33	36.00595714412732	38.0	37.0	38.0	32.0	38.0
34-35	35.972811797431696	38.0	37.0	38.0	32.0	38.0
36-37	35.943816763835436	38.0	36.5	38.0	32.0	38.0
38-39	35.72905790750549	38.0	36.0	38.0	31.0	38.0
40-41	35.884032567414806	38.0	36.0	38.0	31.5	38.0
42-43	35.91762565255497	38.0	36.5	38.0	32.0	38.0
44-45	35.90336788222891	38.0	36.0	38.0	32.0	38.0
46-47	35.908569269265456	38.0	37.0	38.0	32.0	38.0
48-49	35.89396855034359	38.0	36.5	38.0	31.5	38.0
50-51	35.79162379809219	38.0	36.0	38.0	31.0	38.0
52-53	35.813620139973835	38.0	36.0	38.0	31.0	38.0
54-55	35.82855110569167	38.0	36.0	38.0	31.0	38.0
56-57	35.770672179247796	38.0	36.0	38.0	31.0	38.0
58-59	35.757227324111824	38.0	36.0	38.0	31.0	38.0
60-61	35.730054998793335	38.0	36.0	38.0	31.0	38.0
62-63	35.69528382171754	38.0	36.0	38.0	31.0	38.0
64-65	35.659394886255384	38.0	36.0	38.0	31.0	38.0
66-67	35.591783205680244	38.0	36.0	38.0	31.0	38.0
68-69	35.48091872118279	38.0	36.0	38.0	31.0	38.0
70-71	35.25314687091161	38.0	36.0	38.0	28.5	38.0
72-73	35.07594088582352	38.0	36.0	38.0	27.0	38.0
74-75	35.086146781999005	38.0	36.0	38.0	27.0	38.0
76	34.213155254099505	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	2.0
21	6.0
22	24.0
23	54.0
24	113.0
25	275.0
26	496.0
27	953.0
28	1469.0
29	2271.0
30	3451.0
31	5058.0
32	6957.0
33	9701.0
34	12981.0
35	17985.0
36	26634.0
37	69026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.182842408769325	17.075664621676893	9.325026356234678	28.41646661331911
2	23.894625868485562	18.782786520850003	31.86373509126243	25.458852519401997
3	20.431480140735943	20.743944416923878	29.762222306900888	29.062353135439288
4	14.569599512250885	18.07402608949688	34.1894346428889	33.16693975536333
5	14.507360693010199	32.51851287327414	36.09406952965235	16.880056904063306
6	31.504274155647856	37.479835892746	16.275451231439494	14.74043872016665
7	29.567884769271807	27.627049752949993	20.069478845152357	22.735586632625843
8	25.861499574489706	35.46850588728423	17.904457061565623	20.76553747666044
9	24.503677171055138	14.78108447967077	19.076833187262636	41.63840516201145
10-11	26.01233344764953	24.294415018608138	26.58677234564138	23.106479188100952
12-13	25.576661712964725	21.129444042220783	25.961843793265505	27.332050451548984
14-15	23.655514486402723	21.90933455270612	21.945852227260605	32.489298733630555
16-17	26.025352792490697	26.242871114836973	22.689860153183705	25.041915939488625
18-19	25.636995262228655	24.935220820790306	23.505315703235148	25.922468213745887
20-21	23.692984795945584	24.650382959265325	23.726962110531062	27.92967013425802
22-23	24.29663783358102	25.70399725641123	24.064194896416822	25.935170013590923
24-25	25.703679711415106	25.845304779687282	23.917171563210506	24.533843945687103
26-27	24.8910820663288	26.3679838433106	24.22963583939844	24.51129825096216
28-29	25.443610359587954	24.434769906895806	24.73484992823483	25.386769805281407
30-31	24.396029417368442	27.422550775444883	24.16231630021974	24.01910350696694
32-33	23.23286209655908	26.77888706829758	25.579202072933732	24.409048762209608
34-35	26.092354786673273	24.038791296726746	24.547815925516645	25.321037991083333
36-37	24.193435709840085	25.275311511641203	23.89272059850881	26.638532180009904
38-39	23.402113579494213	24.835511692006758	26.20762362026699	25.554751108232036
40-41	23.49483671836299	23.44879269392473	27.080872359613355	25.97549822809892
42-43	25.155597048101715	23.70028833085648	26.410217327795348	24.733897293246454
44-45	22.757497237358535	25.50489654384026	27.01958617536105	24.718020043440156
46-47	24.740248193168974	23.831751959252625	25.496957918937113	25.931041928641292
48-49	24.227413024425562	23.558345717588182	25.724955226155544	26.489286031830712
50-51	22.416771456515388	23.77935703489184	27.302518766909273	26.501352741683498
52-53	22.144000304843196	23.58120895730925	29.183655323959407	25.09113541388815
54-55	21.556224517014062	23.729184925503944	28.058275857689036	26.65631469979296
56-57	23.047415818821527	23.787295659794992	26.197144635394835	26.968143885988642
58-59	21.696261860305604	23.807618539547054	28.782278448856204	25.71384115129114
60-61	23.11918098794599	25.316592361137573	25.62556364236812	25.938663008548314
62-63	20.746802321889014	27.243455397629845	25.63826544221316	26.37147683826798
64-65	21.116424697379617	27.462243899960626	25.11368110861309	26.307650294046663
66-67	21.296155165186907	27.46573689491801	24.499231541109374	26.73887639878571
68-69	21.144368657038704	27.809638125722415	24.197563794789723	26.84842942244916
70-71	21.727698814922075	27.084047809574617	24.322358978267218	26.86589439723609
72-73	21.8613852582911	25.784018595434972	24.322994068259472	28.031602078014455
74-75	21.652123105844097	25.83831878977251	24.70500069859899	27.804557405784397
76	22.139872219893558	25.437259459665434	24.497326271132618	27.925542049308387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	2.0
15	13.0
16	30.0
17	38.0
18	48.0
19	67.0
20	97.5
21	119.0
22	144.0
23	229.0
24	357.0
25	425.0
26	522.5
27	680.5
28	859.5
29	978.0
30	1140.0
31	1490.0
32	1989.0
33	2300.0
34	2572.0
35	3158.0
36	3832.0
37	4192.0
38	4683.0
39	5817.0
40	7147.5
41	8553.5
42	9272.0
43	9556.5
44	9760.5
45	9335.5
46	8991.0
47	8728.5
48	7967.5
49	6991.5
50	6514.0
51	6109.5
52	5146.5
53	4376.0
54	4164.0
55	3868.5
56	3319.5
57	2832.0
58	2598.0
59	2449.5
60	2253.5
61	2153.0
62	2100.0
63	2107.5
64	2130.0
65	2150.5
66	2241.0
67	2326.0
68	2273.0
69	2246.0
70	2236.0
71	2200.0
72	2197.0
73	2183.0
74	2011.5
75	1851.0
76	1749.5
77	1498.0
78	1217.5
79	1087.0
80	953.0
81	699.0
82	503.5
83	428.0
84	357.5
85	201.0
86	97.5
87	80.0
88	60.0
89	32.0
90	19.0
91	9.0
92	4.0
93	2.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	157458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.61968270903987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.93244955388698	30.920626452768357
2	18.83277096864539	17.93621156117822
3	6.808391459169656	9.726403231337882
4	2.7060188581107214	5.154390377116438
5	1.5563942865525933	3.705750104789849
6	0.9722463023966071	2.7778836261098196
7	0.7095130766460837	2.3650751311460834
8	0.5134634107307184	1.9560771761358584
9	0.46011656286259184	1.971954425942156
>10	2.475293741081074	19.611578960738736
>50	0.02533975273736013	0.7252727711516722
>100	0.006668355983515823	0.713841151291138
>500	0.0	0.0
>1k	0.0013336711967031649	2.4349350302937927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT	3834	2.4349350302937927	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG	385	0.2445096470169823	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT	248	0.1575023180784717	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC	229	0.14543560822568558	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT	161	0.10224948875255625	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.350899922519021E-4	0.0
2	0.0	0.0	0.0	6.350899922519021E-4	0.0
3	0.0	0.0	0.0	6.350899922519021E-4	0.0
4	0.0	0.0	0.0	0.0012701799845038042	0.0
5	0.0	0.0	0.0	0.0025403599690076084	0.0
6	0.0	0.0	0.0	0.0025403599690076084	0.0
7	0.0	0.0	0.0	0.0025403599690076084	0.0
8	0.0	0.0	0.0	0.0025403599690076084	0.0
9	0.0	0.0	0.0	0.0044456299457633144	0.0
10	0.0	0.0	0.0	0.0044456299457633144	0.0
11	0.0	0.0	0.0	0.005080719938015217	0.0
12	0.0	0.0	0.0	0.005080719938015217	0.0
13	0.0	0.0	0.0	0.005080719938015217	0.0
14	0.0	0.0	0.0	0.005080719938015217	0.0
15	0.0	0.0	0.0	0.005080719938015217	0.0
16	0.0	0.0	0.0	0.005080719938015217	0.0
17	0.0	0.0	0.0	0.005715809930267119	0.0
18	0.0	0.0	0.0	0.005715809930267119	0.0
19	0.0	0.0	0.0	0.008891259891526629	0.0
20	0.0	0.0	0.0	0.008891259891526629	0.0
21	0.0	0.0	0.0	0.008891259891526629	0.0
22	0.0	0.0	0.0	0.010161439876030433	0.0
23	0.0	0.0	0.0	0.011431619860534238	0.0
24	0.0	0.0	0.0	0.01206670985278614	0.0
25	0.0	0.0	0.0	0.013971979829541846	0.0
26	0.0	0.0	0.0	0.015877249806297553	0.0
27	0.0	0.0	0.0	0.0311194096203432	0.0
28	0.0	0.0	0.0	0.04953701939564836	0.0
29	0.0	0.0	0.0	0.07113007913221303	0.0
30	0.0	0.0	0.0	0.0895476889075182	0.0
31	0.0	0.0	0.0	0.11050565865183097	0.0
32	0.0	0.0	0.0	0.13844961831091465	0.0
33	0.0	0.0	0.0	0.18671645772205922	0.0
34	0.0	0.0	0.0	0.272453606676066	0.0
35	0.0	0.0	0.0	0.382959265327897	0.0
36	0.0	0.0	0.0	0.6001600426780475	0.0
37	0.0	0.0	0.0	0.8668978394238464	0.0
38	0.0	0.0	0.0	1.2168324251546445	0.0
39	0.0	0.0	0.0	1.550254671086893	0.0
40	0.0	0.0	0.0	1.945280646267576	0.0
41	0.0	0.0	0.0	2.349197881339786	0.0
42	0.0	0.0	0.0	2.756290566373255	0.0
43	0.0	0.0	0.0	3.2700783701050438	0.0
44	0.0	0.0	0.0	3.787676713790344	0.0
45	0.0	0.0	0.0	4.4316579659337725	0.0
46	0.0	0.0	0.0	5.142323667263652	0.0
47	0.0	0.0	0.0	5.8802982382603615	0.0
48	0.0	0.0	0.0	6.597949929505011	0.0
49	0.0	0.0	0.0	7.284482211129317	0.0
50	0.0	0.0	0.0	7.896073873667898	0.0
51	0.0	0.0	0.0	8.536879675850068	0.0
52	0.0	0.0	0.0	9.144025708442886	0.0
53	0.0	0.0	0.0	9.714971611477347	0.0
54	0.0	0.0	0.0	10.319577284101157	0.0
55	0.0	0.0	0.0	10.967369076198096	0.0
56	0.0	0.0	0.0	11.67612950755122	0.0
57	0.0	0.0	0.0	12.373458319043808	0.0
58	0.0	0.0	0.0	13.081583660404679	0.0
59	0.0	0.0	0.0	13.77002121200574	0.0
60	0.0	0.0	0.0	14.40384102427314	0.0
61	0.0	0.0	0.0	14.969706207369585	0.0
62	0.0	0.0	0.0	15.566690800086372	0.0
63	0.0	0.0	0.0	16.14652796301236	0.0
64	0.0	0.0	0.0	16.728905485907354	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACA	15	0.00222133	70.00001	44
GATCTTA	15	0.00222133	70.00001	1
TATGCCG	535	0.0	57.57009	43
CGTATGC	545	0.0	56.513763	41
TCGTATG	545	0.0	56.513763	40
CTCGTAT	530	0.0	56.132076	39
CTTGAAA	505	0.0	55.445545	57
GTATGCC	560	0.0	55.0	42
CCGTCTT	505	0.0	54.752476	47
TCTCGTA	500	0.0	54.6	38
ATGCCGT	535	0.0	54.299065	44
CGTCTTC	505	0.0	54.059406	48
ATCTCGT	500	0.0	53.899998	37
GCCGTCT	540	0.0	53.148148	46
TGCCGTC	550	0.0	52.818184	45
CATCTCG	505	0.0	52.673267	36
CTCGAAA	20	0.006940956	52.499996	57
GGGCAGT	20	0.006940956	52.499996	37
ATGCAAG	20	0.006940956	52.499996	38
GCTTGAA	535	0.0	52.336445	56
>>END_MODULE