FastQCFastQC Report
Wed 25 May 2016
SRR1781159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166847
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG252.3850954E-455.99999614
TATCGGC252.3850954E-455.99999615
AAACGCA200.006941674752.537
TCGTGGG200.006941674752.52
ACCGTAT407.4454874E-752.511
TATCGGA200.006941674752.517
AACCGTA352.0797357E-550.010
GCCTTAG352.0797357E-550.040
CGTATCG305.8648805E-446.66666413
CAACCGT404.5778503E-543.759
ATCGGTT404.5778503E-543.7525
TATCGGT404.5778503E-543.7524
CCGTATC350.001252685640.012
TTAGGTG400.00241352935.049
GACAAAC400.00241352935.052
CAACCTG400.00241352935.040
CGACAAA400.00241352935.051
CGCTATG400.00241352935.024
TACGACA400.00241352935.049
GCTAGTC400.00241352935.07
Produced by FastQC (version 0.11.5)