##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781159_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166847
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204127134440537	34.0	32.0	34.0	23.0	34.0
2	31.272806823017493	34.0	33.0	34.0	23.0	34.0
3	31.258464341582407	34.0	33.0	34.0	23.0	34.0
4	31.42428692155088	34.0	33.0	34.0	23.0	34.0
5	31.364897181249887	34.0	33.0	34.0	23.0	34.0
6	34.111407457131385	37.0	34.0	38.0	24.0	38.0
7	34.06374702571817	37.0	34.0	38.0	24.0	38.0
8	34.00506452018916	37.0	34.0	38.0	24.0	38.0
9	34.29851900243936	37.0	34.0	38.0	24.0	38.0
10-11	34.13415584337747	37.0	34.0	38.0	24.0	38.0
12-13	34.09228814422795	37.0	34.0	38.0	23.5	38.0
14-15	33.958300119270945	37.0	34.0	38.0	23.0	38.0
16-17	33.933067421050424	37.0	34.0	38.0	23.0	38.0
18-19	33.97658034007204	37.0	34.0	38.0	23.0	38.0
20-21	33.89642606699551	37.0	34.0	38.0	23.0	38.0
22-23	33.97884888550588	37.0	34.0	38.0	23.5	38.0
24-25	33.96380815957134	37.0	34.0	38.0	23.0	38.0
26-27	33.86621875131108	37.0	34.0	38.0	23.0	38.0
28-29	33.98090466115663	37.0	34.0	38.0	23.0	38.0
30-31	34.050327545595664	37.0	34.0	38.0	23.5	38.0
32-33	34.00359910576756	37.0	34.0	38.0	23.5	38.0
34-35	34.03526883911608	37.0	34.0	38.0	23.0	38.0
36-37	33.88786433079408	37.0	34.0	38.0	23.0	38.0
38-39	33.89274305201772	37.0	34.0	38.0	23.0	38.0
40-41	33.89775363057173	37.0	34.0	38.0	23.0	38.0
42-43	33.98573243750262	37.0	34.0	38.0	23.0	38.0
44-45	33.99450094997213	37.0	34.0	38.0	23.5	38.0
46-47	33.977296565116546	37.0	34.0	38.0	23.5	38.0
48-49	33.9554202353054	37.0	34.0	38.0	23.0	38.0
50-51	33.83175304320725	37.0	34.0	38.0	23.0	38.0
52-53	33.90060354696219	37.0	34.0	38.0	23.0	38.0
54-55	33.88095980149478	37.0	34.0	38.0	23.0	38.0
56-57	33.84150449213951	37.0	34.0	38.0	23.0	38.0
58-59	33.85362038274587	37.0	34.0	38.0	23.0	38.0
60-61	33.81803388733391	37.0	34.0	38.0	23.0	38.0
62-63	33.79746714055392	37.0	34.0	38.0	23.0	38.0
64-65	33.8035086036908	37.0	34.0	38.0	23.0	38.0
66-67	33.69095638519122	37.0	34.0	38.0	22.5	38.0
68-69	33.4949204960233	37.0	34.0	38.0	21.5	38.0
70-71	33.64769819055782	37.0	34.0	38.0	22.5	38.0
72-73	33.64730861208173	37.0	34.0	38.0	22.5	38.0
74-75	33.64843838966239	37.0	34.0	38.0	22.5	38.0
76	32.395494075410404	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	26.0
14	86.0
15	272.0
16	614.0
17	1147.0
18	1425.0
19	1560.0
20	1550.0
21	1475.0
22	1506.0
23	1798.0
24	1910.0
25	2431.0
26	2857.0
27	3331.0
28	3829.0
29	4621.0
30	5388.0
31	6441.0
32	7832.0
33	9474.0
34	12196.0
35	16028.0
36	24558.0
37	54488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.87376458671717	19.200824707666307	9.37146008019323	25.553950625423294
2	22.698040719941023	20.46785378220765	32.258895874663615	24.57520962318771
3	19.205619519679708	22.52902359646862	30.03470245194699	28.23065443190468
4	13.659820074679196	16.706923109195852	38.10137431299334	31.531882503131612
5	13.400900225955517	34.24814350872356	36.372245230660425	15.978711034660497
6	29.903623700897835	37.330232669647465	18.259952291334525	14.506191338120184
7	28.598656253933246	28.729314881298436	23.490982756657296	19.181046108111026
8	25.638459187159494	34.38359694810215	19.815159996883374	20.16278386785498
9	24.665711699940665	15.205547597499505	21.6719509490731	38.456789753486724
10-11	25.340881166577763	24.512577391262656	28.20158588407343	21.94495555808615
12-13	25.216815405730998	22.64499811204277	27.036446564816867	25.101739917409365
14-15	23.360324129292106	24.086738149322436	24.354348594820404	28.198589126565054
16-17	23.73851492684915	26.737070489730115	25.495813529760795	24.02860105365994
18-19	23.147554346197413	26.38854759150599	26.723285405191582	23.74061265710501
20-21	22.953064783903816	26.67054247304417	26.614802783388374	23.76158995966364
22-23	23.37800499859152	26.041523072036053	27.043938458587807	23.536533470784608
24-25	23.05345616043441	26.362475801183123	26.8230774302205	23.76099060816197
26-27	22.333035655420836	26.775428985837323	26.903390531444977	23.988144827296864
28-29	22.978237546974174	26.343895904631186	27.671759156592568	23.006107391802068
30-31	23.477497347869605	26.253993179379908	26.975013035895163	23.293496436855325
32-33	22.851474704369874	26.639076519206217	26.821279375715473	23.688169400708432
34-35	23.74840422662679	25.67951476502424	27.157815243906093	23.414265764442874
36-37	23.670488531409013	25.658537462465613	26.629486895179415	24.041487110945955
38-39	22.846080540854796	26.36487320718982	27.470077376278866	23.318968875676518
40-41	23.131371855652183	25.631266969139393	27.757466421332115	23.47989475387631
42-43	22.73729824330075	25.935737531990387	27.366689242239893	23.960274982468967
44-45	22.310859649858852	25.9366365592429	27.649583151030583	24.102920639867666
46-47	22.559290847303217	25.83834291296817	27.644488663266348	23.957877576462266
48-49	21.93117047354762	25.579123388493652	28.358316301761494	24.131389836197233
50-51	22.162819828945082	25.58901268827129	27.948959226117342	24.299208256666287
52-53	21.80440763094332	25.47423687570049	28.29598374558727	24.425371747768914
54-55	21.252105222149634	25.93124239572782	28.361013383519033	24.45563899860351
56-57	21.425317806133766	25.930942719976986	27.99271188573963	24.65102758814962
58-59	21.567663787781623	25.794290577595042	27.924385814548657	24.713659820074678
60-61	21.348001462417663	25.61268707258746	28.07362433846578	24.965687126529097
62-63	21.030045490778974	25.14339484677579	28.715829472510745	25.11073018993449
64-65	21.725293232722194	25.64055691741536	27.737987497527676	24.896162352334773
66-67	21.763651728829405	25.458953412407777	27.41703476838061	25.360360090382205
68-69	21.249408140392095	25.696596282821986	27.410741577613024	25.643253999172895
70-71	21.955444209365467	25.3291938122951	27.80481518996446	24.91054678837498
72-73	22.3000713228287	24.686689002499296	27.42662439240742	25.586615282264592
74-75	22.09059797299322	25.154482849556782	27.57586291632454	25.17905626112546
76	22.639304272776855	24.814950223857785	27.387366869047693	25.158378634317668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	2.5
14	8.5
15	13.5
16	24.5
17	35.0
18	41.5
19	69.0
20	120.0
21	150.0
22	184.0
23	292.0
24	468.0
25	570.0
26	677.0
27	858.5
28	1096.5
29	1260.0
30	1401.5
31	1793.5
32	2445.0
33	2846.0
34	3201.5
35	4001.5
36	4972.5
37	5499.0
38	6071.5
39	7390.5
40	8859.5
41	9826.5
42	10071.0
43	10280.5
44	10566.0
45	10578.5
46	10515.0
47	10544.5
48	9864.0
49	8597.5
50	8041.0
51	7561.0
52	6307.0
53	5182.0
54	4831.0
55	4483.0
56	3801.5
57	3059.5
58	2651.0
59	2308.5
60	1899.5
61	1674.5
62	1516.0
63	1514.5
64	1430.0
65	1350.0
66	1302.0
67	1251.0
68	1253.5
69	1247.0
70	1214.0
71	1190.0
72	1149.5
73	1060.0
74	1011.0
75	1011.0
76	907.0
77	740.0
78	602.5
79	528.0
80	475.5
81	378.5
82	266.5
83	199.0
84	167.0
85	109.5
86	66.0
87	48.0
88	34.5
89	17.0
90	9.5
91	4.0
92	2.0
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	5.993515016751874E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	166847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.974245865973025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94913520441158	37.69501399485756
2	12.33641782972169	12.576791911152133
3	4.85367258874296	7.42236899674552
4	2.1810956037108022	4.447188142429891
5	1.3897870639278533	3.5421673749003575
6	0.9206457453938319	2.8157533548700306
7	0.6114122447059931	2.1816394660976823
8	0.5420404707874285	2.2104083381780915
9	0.44680125574668716	2.0497821357291413
>10	2.734894002281038	24.013017914616384
>50	0.034097990570141914	1.0458683704232021
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0023974060067007497	0.0
5	0.0	0.0	0.0	0.0023974060067007497	0.0
6	0.0	0.0	0.0	0.0029967575083759373	0.0
7	0.0	0.0	0.0	0.0029967575083759373	0.0
8	0.0	0.0	0.0	0.0029967575083759373	0.0
9	0.0	0.0	0.0	0.0029967575083759373	0.0
10	0.0	0.0	0.0	0.0029967575083759373	0.0
11	0.0	0.0	0.0	0.0029967575083759373	0.0
12	0.0	0.0	0.0	0.0035961090100511245	0.0
13	0.0	0.0	0.0	0.0035961090100511245	0.0
14	0.0	0.0	0.0	0.004195460511726312	0.0
15	0.0	0.0	0.0	0.004195460511726312	0.0
16	0.0	0.0	0.0	0.004195460511726312	0.0
17	0.0	0.0	0.0	0.004195460511726312	0.0
18	0.0	0.0	0.0	0.004794812013401499	0.0
19	0.0	0.0	0.0	0.005394163515076687	0.0
20	0.0	0.0	0.0	0.006592866518427062	0.0
21	0.0	0.0	0.0	0.008390921023452624	0.0
22	0.0	0.0	0.0	0.01198703003350375	0.0
23	0.0	0.0	0.0	0.014384436040204498	0.0
24	0.0	0.0	0.0	0.01678184204690525	0.0
25	0.0	0.0	0.0	0.01678184204690525	0.0
26	0.0	0.0	0.0	0.01678184204690525	0.0
27	0.0	0.0	0.0	0.02577211457203306	0.0
28	0.0	0.0	0.0	0.03895784760888719	0.0
29	0.0	0.0	0.0	0.047348768632339805	0.0
30	0.0	0.0	0.0	0.0623325561742195	0.0
31	0.0	0.0	0.0	0.08151180422782549	0.0
32	0.0	0.0	0.0	0.1000917007797563	0.0
33	0.0	0.0	0.0	0.13964889989031867	0.0
34	0.0	0.0	0.0	0.182202856509257	0.0
35	0.0	0.0	0.0	0.2721055817605351	0.0
36	0.0	0.0	0.0	0.4105557786475034	0.0
37	0.0	0.0	0.0	0.6161333437220927	0.0
38	0.0	0.0	0.0	0.8396914538469377	0.0
39	0.0	0.0	0.0	1.1129957386108231	0.0
40	0.0	0.0	0.0	1.3958896474015117	0.0
41	0.0	0.0	0.0	1.6979628042458061	0.0
42	0.0	0.0	0.0	2.0635672202676703	0.0
43	0.0	0.0	0.0	2.4771197564235496	0.0
44	0.0	0.0	0.0	2.9937607508675614	0.0
45	0.0	0.0	0.0	3.5541544049338616	0.0
46	0.0	0.0	0.0	4.146913040090622	0.0
47	0.0	0.0	0.0	4.7762321168495685	0.0
48	0.0	0.0	0.0	5.398958327090089	0.0
49	0.0	0.0	0.0	5.94796430262456	0.0
50	0.0	0.0	0.0	6.447224103519991	0.0
51	0.0	0.0	0.0	6.996829430556138	0.0
52	0.0	0.0	0.0	7.486499607424767	0.0
53	0.0	0.0	0.0	7.955791833236439	0.0
54	0.0	0.0	0.0	8.501201699760859	0.0
55	0.0	0.0	0.0	9.109543473961175	0.0
56	0.0	0.0	0.0	9.701103406114584	0.0
57	0.0	0.0	0.0	10.27588149622109	0.0
58	0.0	0.0	0.0	10.829682283768962	0.0
59	0.0	0.0	0.0	11.405059725377141	0.0
60	0.0	0.0	0.0	11.975043003470246	0.0
61	0.0	0.0	0.0	12.461117071328822	0.0
62	0.0	0.0	0.0	12.909431994581862	0.0
63	0.0	0.0	0.0	13.403297631962216	0.0
64	0.0	0.0	0.0	13.946909443981612	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGG	25	2.3850954E-4	55.999996	14
TATCGGC	25	2.3850954E-4	55.999996	15
AAACGCA	20	0.0069416747	52.5	37
TCGTGGG	20	0.0069416747	52.5	2
ACCGTAT	40	7.4454874E-7	52.5	11
TATCGGA	20	0.0069416747	52.5	17
AACCGTA	35	2.0797357E-5	50.0	10
GCCTTAG	35	2.0797357E-5	50.0	40
CGTATCG	30	5.8648805E-4	46.666664	13
CAACCGT	40	4.5778503E-5	43.75	9
ATCGGTT	40	4.5778503E-5	43.75	25
TATCGGT	40	4.5778503E-5	43.75	24
CCGTATC	35	0.0012526856	40.0	12
TTAGGTG	40	0.002413529	35.0	49
GACAAAC	40	0.002413529	35.0	52
CAACCTG	40	0.002413529	35.0	40
CGACAAA	40	0.002413529	35.0	51
CGCTATG	40	0.002413529	35.0	24
TACGACA	40	0.002413529	35.0	49
GCTAGTC	40	0.002413529	35.0	7
>>END_MODULE