FastQCFastQC Report
Wed 25 May 2016
SRR1781159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149790
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT33362.2271179651512116No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG3380.22564924227251484No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC2440.16289471927364976No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT2150.1435342813271914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACG150.002221117770.062
TATCGTC207.906156E-570.013
CGTCACA207.906156E-570.016
GTTATCG207.906156E-570.011
TATGCCG4600.056.30434443
CGTTATC252.3843044E-456.00000410
ATCGTCA252.3843044E-456.00000414
TCGTCAC252.3843044E-456.00000415
CGTATGC4600.055.54347641
CCGTCTT4300.055.3488447
TCGTATG4650.054.94623640
CTCGTAT4400.054.8863639
GCCGTCT4400.054.09090846
CGTCTTC4350.053.90804748
GTATGCC4750.053.7894742
ATCTCGT4150.053.1325337
ATGCCGT4750.053.05262844
TAGCGAG200.00694029552.528
TAACGAC200.00694029552.564
CGTGTCC200.00694029552.53
Produced by FastQC (version 0.11.5)