##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781159_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149790
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.26931036784832	34.0	34.0	34.0	32.0	34.0
2	33.30911943387409	34.0	34.0	34.0	33.0	34.0
3	33.353928833700515	34.0	34.0	34.0	33.0	34.0
4	33.43274584418185	34.0	34.0	34.0	34.0	34.0
5	33.41483410107484	34.0	34.0	34.0	34.0	34.0
6	36.82004139128113	38.0	37.0	38.0	34.0	38.0
7	36.75211963415448	38.0	37.0	38.0	34.0	38.0
8	36.54389478603378	38.0	37.0	38.0	34.0	38.0
9	36.520775752720475	38.0	37.0	38.0	34.0	38.0
10-11	36.41244742639695	38.0	37.0	38.0	34.0	38.0
12-13	36.344595767407704	38.0	37.0	38.0	34.0	38.0
14-15	36.36428666800187	38.0	37.0	38.0	34.0	38.0
16-17	36.29294345416917	38.0	37.0	38.0	34.0	38.0
18-19	36.289882502169704	38.0	37.0	38.0	34.0	38.0
20-21	36.2341478069297	38.0	37.0	38.0	34.0	38.0
22-23	36.294786033780625	38.0	37.0	38.0	34.0	38.0
24-25	36.28610054075706	38.0	37.0	38.0	34.0	38.0
26-27	36.07020161559517	38.0	37.0	38.0	32.5	38.0
28-29	36.06761132251819	38.0	37.0	38.0	32.5	38.0
30-31	36.096281460711666	38.0	37.0	38.0	33.0	38.0
32-33	36.04193871419988	38.0	37.0	38.0	32.0	38.0
34-35	36.03856732759196	38.0	37.0	38.0	32.5	38.0
36-37	35.98004205888243	38.0	37.0	38.0	32.0	38.0
38-39	35.80609853795313	38.0	36.5	38.0	31.0	38.0
40-41	35.922231123573	38.0	37.0	38.0	32.0	38.0
42-43	35.97826957740837	38.0	37.0	38.0	32.0	38.0
44-45	35.95161225716002	38.0	37.0	38.0	32.0	38.0
46-47	35.96412978169437	38.0	37.0	38.0	32.0	38.0
48-49	35.945830829828424	38.0	37.0	38.0	32.0	38.0
50-51	35.86301154950264	38.0	36.5	38.0	31.5	38.0
52-53	35.8661659656853	38.0	37.0	38.0	31.5	38.0
54-55	35.8904399492623	38.0	37.0	38.0	32.0	38.0
56-57	35.8322151011416	38.0	36.5	38.0	31.5	38.0
58-59	35.814126443687826	38.0	36.0	38.0	31.0	38.0
60-61	35.80832832632352	38.0	36.0	38.0	31.0	38.0
62-63	35.780976700714334	38.0	36.0	38.0	31.0	38.0
64-65	35.75041391281127	38.0	36.0	38.0	31.0	38.0
66-67	35.69596435009012	38.0	36.0	38.0	31.0	38.0
68-69	35.58799319046665	38.0	36.0	38.0	31.0	38.0
70-71	35.40902263168436	38.0	36.0	38.0	29.5	38.0
72-73	35.23416783496896	38.0	36.0	38.0	27.0	38.0
74-75	35.243631083516924	38.0	36.0	38.0	27.0	38.0
76	34.40927965818813	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	3.0
21	7.0
22	19.0
23	51.0
24	109.0
25	211.0
26	431.0
27	796.0
28	1363.0
29	2049.0
30	3228.0
31	4539.0
32	6437.0
33	8998.0
34	12094.0
35	16801.0
36	25417.0
37	67234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45750717671407	18.63609052673743	9.472594966286135	28.43380733026237
2	22.210427932438748	19.789037986514455	33.54896855597837	24.451565525068432
3	19.02797249482609	22.537552573603044	31.030776420321782	27.40369851124908
4	13.587689431871286	18.969891180986714	37.17471126243407	30.267708124707926
5	13.056946391614927	34.279991988784296	37.87435743374057	14.788704185860205
6	29.00727685426264	40.134187863008215	17.178716870285065	13.679818412444089
7	27.630682956138592	29.206222044195208	22.010815141197675	21.15227985846852
8	24.47359636824888	37.2087589291675	18.397089258294947	19.92055544428867
9	23.736564523666466	14.829427865678616	20.73502904065692	40.698978569998
10-11	24.48027238133387	25.696308164764005	28.011883303291274	21.811536150610856
12-13	24.15448294278657	22.82295213298618	27.37666065825489	25.645904265972362
14-15	22.577942452767207	23.895787435743372	23.024901528806996	30.50136858268242
16-17	24.46591895320115	27.834301355230657	24.01862607650711	23.681153615061085
18-19	24.058682155017024	26.32919420522064	25.31243741237733	24.299686227385006
20-21	22.465117831630952	26.16129247613325	25.061085519727616	26.312504172508177
22-23	22.52520194939582	27.156352226450366	25.67427732158355	24.644168502570267
24-25	24.044328726884302	27.47045864209894	25.181587555911612	23.303625075105145
26-27	23.152079578075973	27.678416449696243	25.58448494559049	23.58501902663729
28-29	22.592295880899925	25.876226717404364	26.23973562988183	25.291741771813875
30-31	22.453101008077976	28.701515454970295	25.73502904065692	23.110354496294814
32-33	23.55297416382936	27.852660391214368	25.64356766139262	22.950797783563655
34-35	23.201815875559113	27.090593497563255	27.06422324587756	22.64336738100007
36-37	22.895053074304027	26.57420388543962	27.08057947793578	23.450163562320583
38-39	22.152680419253624	26.30616196007744	27.508845717337604	24.03231190333133
40-41	22.336938380399225	25.068095333466857	28.035249349088726	24.5597169370452
42-43	23.629748314306696	25.085452967487814	27.691100874557712	23.593697843647774
44-45	21.724080379197545	27.03151078176113	27.81327191401295	23.431136925028373
46-47	23.399092062220443	25.120168235529743	26.768142065558447	24.71259763669137
48-49	22.779558047933772	24.970959343080313	27.07857667401028	25.170905934975636
50-51	21.62360638226851	25.122838640763735	28.28526603912144	24.968288937846317
52-53	21.081514119767675	24.861472728486547	30.306095199946594	23.750917951799185
54-55	20.547433072968822	25.21296481741104	29.004606449028643	25.234995660591498
56-57	21.93337338941184	25.234328059282994	27.33994258628747	25.492355965017694
58-59	20.839842446091193	24.849789705587824	29.723613058281593	24.58675479003939
60-61	22.1863942853328	26.425328793644436	27.03985579811736	24.3484211229054
62-63	19.667534548367716	28.093664463582346	27.130315775418918	25.108485212631017
64-65	20.155884905534414	28.8146738767608	26.227051205020363	24.802390012684423
66-67	20.313105013685824	28.379731624273987	26.039455237332266	25.267708124707926
68-69	19.959276320181587	29.08438480539422	25.648574671206354	25.307764203217836
70-71	20.310434608451832	28.18946525135189	25.68796314840777	25.812136991788503
72-73	20.477334935576476	26.87796248080646	25.864877495159888	26.77982508845717
74-75	20.51939381801188	27.17804926897657	25.768409106081847	26.534147806929703
76	20.988717537886377	27.141331197009144	25.5350824487616	26.334868816342883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	3.5
15	12.0
16	22.0
17	26.0
18	40.0
19	71.0
20	107.5
21	127.0
22	159.5
23	257.5
24	402.5
25	482.0
26	555.0
27	736.5
28	1030.0
29	1215.0
30	1313.0
31	1564.0
32	2163.0
33	2609.0
34	2883.0
35	3483.5
36	4288.0
37	4766.0
38	5347.0
39	7058.5
40	8872.0
41	9645.0
42	9735.0
43	9751.0
44	9642.5
45	9356.5
46	9195.0
47	9153.5
48	8450.5
49	7426.5
50	7064.0
51	6491.5
52	5389.0
53	4591.5
54	4324.0
55	3971.5
56	3283.0
57	2625.5
58	2304.0
59	2026.5
60	1568.5
61	1383.5
62	1379.0
63	1271.0
64	1172.0
65	1150.5
66	1158.0
67	1196.0
68	1210.5
69	1136.0
70	1077.5
71	1108.0
72	1089.5
73	1012.0
74	903.0
75	853.0
76	830.0
77	692.0
78	535.5
79	494.0
80	444.5
81	350.5
82	247.0
83	188.0
84	156.0
85	104.0
86	65.0
87	46.0
88	32.0
89	15.5
90	8.5
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	149790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.537485813472195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.06433726032241	26.19534014286668
2	17.853067390989892	14.831430669604112
3	6.901428823992671	8.60004005607851
4	3.1871293334833415	5.2954135790106145
5	1.7470547581928348	3.628413111689699
6	1.2391713142287726	3.0883236531143603
7	0.9080827399990357	2.6403631751118235
8	0.7537890355036243	2.5048401094866146
9	0.5528857744418908	2.066893651111556
>10	3.719121168774812	26.57587288871086
>50	0.06428904353975473	1.6576540490019358
>100	0.008036130442469341	0.6882969490620202
>500	0.0	0.0
>1k	0.0016072260884938683	2.2271179651512116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	3336	2.2271179651512116	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	338	0.22564924227251484	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	244	0.16289471927364976	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTACCTCGTATGCCGT	215	0.1435342813271914	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.676013084985647E-4	0.0
2	0.0	0.0	0.0	6.676013084985647E-4	0.0
3	0.0	0.0	0.0	6.676013084985647E-4	0.0
4	0.0	0.0	0.0	0.0026704052339942587	0.0
5	0.0	0.0	0.0	0.0026704052339942587	0.0
6	0.0	0.0	0.0	0.0033380065424928234	0.0
7	0.0	0.0	0.0	0.0033380065424928234	0.0
8	0.0	0.0	0.0	0.0033380065424928234	0.0
9	0.0	0.0	0.0	0.004005607850991388	0.0
10	0.0	0.0	0.0	0.004005607850991388	0.0
11	0.0	0.0	0.0	0.004005607850991388	0.0
12	0.0	0.0	0.0	0.004673209159489953	0.0
13	0.0	0.0	0.0	0.004673209159489953	0.0
14	0.0	0.0	0.0	0.005340810467988517	0.0
15	0.0	0.0	0.0	0.005340810467988517	0.0
16	0.0	0.0	0.0	0.005340810467988517	0.0
17	0.0	0.0	0.0	0.005340810467988517	0.0
18	0.0	0.0	0.0	0.006008411776487082	0.0
19	0.0	0.0	0.0	0.006008411776487082	0.0
20	0.0	0.0	0.0	0.008011215701982776	0.0
21	0.0	0.0	0.0	0.008011215701982776	0.0
22	0.0	0.0	0.0	0.010681620935977035	0.0
23	0.0	0.0	0.0	0.012016823552974164	0.0
24	0.0	0.0	0.0	0.012684424861472728	0.0
25	0.0	0.0	0.0	0.013352026169971293	0.0
26	0.0	0.0	0.0	0.014019627478469857	0.0
27	0.0	0.0	0.0	0.025368849722945456	0.0
28	0.0	0.0	0.0	0.04005607850991388	0.0
29	0.0	0.0	0.0	0.051405300754389475	0.0
30	0.0	0.0	0.0	0.06742773215835503	0.0
31	0.0	0.0	0.0	0.08812337272181053	0.0
32	0.0	0.0	0.0	0.10881901328526604	0.0
33	0.0	0.0	0.0	0.15021029441217704	0.0
34	0.0	0.0	0.0	0.2022831964750651	0.0
35	0.0	0.0	0.0	0.30108819013285265	0.0
36	0.0	0.0	0.0	0.4573068963215168	0.0
37	0.0	0.0	0.0	0.6869617464450231	0.0
38	0.0	0.0	0.0	0.924627812270512	0.0
39	0.0	0.0	0.0	1.207023165765405	0.0
40	0.0	0.0	0.0	1.4987649375792778	0.0
41	0.0	0.0	0.0	1.8232191735095802	0.0
42	0.0	0.0	0.0	2.2130983376727418	0.0
43	0.0	0.0	0.0	2.653047599973296	0.0
44	0.0	0.0	0.0	3.1904666533146404	0.0
45	0.0	0.0	0.0	3.7873022231123574	0.0
46	0.0	0.0	0.0	4.391481407303559	0.0
47	0.0	0.0	0.0	5.038387075238668	0.0
48	0.0	0.0	0.0	5.687963148407771	0.0
49	0.0	0.0	0.0	6.256759463248548	0.0
50	0.0	0.0	0.0	6.775485679951933	0.0
51	0.0	0.0	0.0	7.352293210494692	0.0
52	0.0	0.0	0.0	7.856999799719607	0.0
53	0.0	0.0	0.0	8.345016356232058	0.0
54	0.0	0.0	0.0	8.918485880232325	0.0
55	0.0	0.0	0.0	9.555377528539957	0.0
56	0.0	0.0	0.0	10.163562320582148	0.0
57	0.0	0.0	0.0	10.791775151879298	0.0
58	0.0	0.0	0.0	11.37192068896455	0.0
59	0.0	0.0	0.0	11.99479270979371	0.0
60	0.0	0.0	0.0	12.587622671740437	0.0
61	0.0	0.0	0.0	13.096334868816342	0.0
62	0.0	0.0	0.0	13.589024634488284	0.0
63	0.0	0.0	0.0	14.104412844649175	0.0
64	0.0	0.0	0.0	14.681887976500434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAACG	15	0.0022211177	70.0	62
TATCGTC	20	7.906156E-5	70.0	13
CGTCACA	20	7.906156E-5	70.0	16
GTTATCG	20	7.906156E-5	70.0	11
TATGCCG	460	0.0	56.304344	43
CGTTATC	25	2.3843044E-4	56.000004	10
ATCGTCA	25	2.3843044E-4	56.000004	14
TCGTCAC	25	2.3843044E-4	56.000004	15
CGTATGC	460	0.0	55.543476	41
CCGTCTT	430	0.0	55.34884	47
TCGTATG	465	0.0	54.946236	40
CTCGTAT	440	0.0	54.88636	39
GCCGTCT	440	0.0	54.090908	46
CGTCTTC	435	0.0	53.908047	48
GTATGCC	475	0.0	53.78947	42
ATCTCGT	415	0.0	53.13253	37
ATGCCGT	475	0.0	53.052628	44
TAGCGAG	20	0.006940295	52.5	28
TAACGAC	20	0.006940295	52.5	64
CGTGTCC	20	0.006940295	52.5	3
>>END_MODULE